Featured Research

from universities, journals, and other organizations

Manufacturing new gut to treat GI diseases

Date:
December 3, 2013
Source:
Brigham and Women's Hospital
Summary:
Researchers grow extensive numbers of intestinal stem cells, then coax them to develop into different types of mature intestinal cells.

Near homogenous culture of intestinal stem cells.
Credit: Image courtesy of Karp Lab

For those living with gastrointestinal disorders, such as ulcers or Crohn's disease, treatment often means quelling uncomfortable symptoms through medications or dietary changes. But what if one day treatment meant doing away with the old gut for a new gut free of inflamed or diseased tissues?

That is where scientists at Brigham and Women's Hospital (BWH) and Massachusetts Institute of Technology (MIT) are hoping their new study findings will lead. In their work, the researchers were able to grow extensive numbers of intestinal stem cells, then coax them to develop into different types of mature intestinal cells.

The study is published online in this month's Nature Methods.

"Being able to produce a large inventory of intestinal stem cells could be incredibly useful for stem cell therapy, where the cells could be delivered to patients to treat diseases such as Crohn's disease and ulcerative colitis," said Jeffrey Karp, PhD, Division of Biomedical Engineering, BWH Department of Medicine, co-senior study author. "These cells could also be useful for pharmaceutical companies to screen and identify new drugs that could regulate diseases from inflammatory bowel disease, to diabetes, to obesity. However, to date there hasn't been a way to expand intestinal stem cell numbers."

In the "crypts" of the human gut are immature adult stem cells that live alongside specialized cells called Paneth cells. The stem cells remain immature as long as they remain in contact with Paneth cells. But the researchers found that when Paneth cells are removed and replaced with two small molecules involved in cell signaling, these molecules could direct the stem cells to develop into pure populations of proliferating stem cells. By introducing other molecules to the mix, the pure cells could further develop into specialized mature intestinal cells.

"This is an opportunity to generate a large number of relevant mature gastrointestinal cell types that was not possible before and enable high-throughput screening using these cell types," said Xiaolei Yin, PhD, Center for Regenerative Therapeutics, BWH Department of Medicine, lead study author.

Moreover, the researchers note that their findings could be potentially applied for in vivo use of small molecule drugs to help regenerate cells to replace damaged gut tissue caused by disease.

"This opens the door to doing all kinds of things, ranging from someday engineering a new gut for patients with intestinal diseases to doing drug screening for safety and efficacy," said Robert Langer, ScD, MIT, co-senior study author.


Story Source:

The above story is based on materials provided by Brigham and Women's Hospital. Note: Materials may be edited for content and length.


Journal Reference:

  1. Xiaolei Yin, Henner F Farin, Johan H van Es, Hans Clevers, Robert Langer, Jeffrey M Karp. Niche-independent high-purity cultures of Lgr5 intestinal stem cells and their progeny. Nature Methods, 2013; DOI: 10.1038/nmeth.2737

Cite This Page:

Brigham and Women's Hospital. "Manufacturing new gut to treat GI diseases." ScienceDaily. ScienceDaily, 3 December 2013. <www.sciencedaily.com/releases/2013/12/131203112217.htm>.
Brigham and Women's Hospital. (2013, December 3). Manufacturing new gut to treat GI diseases. ScienceDaily. Retrieved September 19, 2014 from www.sciencedaily.com/releases/2013/12/131203112217.htm
Brigham and Women's Hospital. "Manufacturing new gut to treat GI diseases." ScienceDaily. www.sciencedaily.com/releases/2013/12/131203112217.htm (accessed September 19, 2014).

Share This



More Health & Medicine News

Friday, September 19, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Could Grief Affect The Immune Systems Of Senior Citizens?

Could Grief Affect The Immune Systems Of Senior Citizens?

Newsy (Sep. 19, 2014) The study found elderly people are much more likely to become susceptible to infection than younger adults going though a similar situation. Video provided by Newsy
Powered by NewsLook.com
Jury Delivers Verdict in Salmonella Trial

Jury Delivers Verdict in Salmonella Trial

AP (Sep. 19, 2014) A federal jury has convicted three people in connection with an outbreak of salmonella poisoning five years ago that sickened hundreds of people and was linked to a number of deaths. (Sept. 19) Video provided by AP
Powered by NewsLook.com
How The 'Angelina Jolie Effect' Increased Cancer Screenings

How The 'Angelina Jolie Effect' Increased Cancer Screenings

Newsy (Sep. 19, 2014) Angelina's Jolie's decision to undergo a preventative mastectomy in 2013 inspired many women to seek early screenings for the disease. Video provided by Newsy
Powered by NewsLook.com
The Cost of Ebola

The Cost of Ebola

Reuters - Business Video Online (Sep. 18, 2014) As Sierra Leone prepares for a three-day "lockdown" in its latest bid to stem the spread of Ebola, Ciara Lee looks at the financial implications of fighting the largest ever outbreak of the disease. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins