Featured Research

from universities, journals, and other organizations

Research may unlock enzyme's role in disease

Date:
January 2, 2014
Source:
University of Texas at Arlington
Summary:
Research on enzymes that regulate human biology has uncovered characteristics that could be used to identify predisposition to conditions such as heart disease, diabetic ulcers and some types of cancer.

First and Second coordination spheres of the CDO active site.
Credit: University of Texas at Arlington

A UT Arlington chemist doing National Science Foundation-funded research on enzymes that regulate human biology has uncovered characteristics that could be used to identify predisposition to conditions such as heart disease, diabetic ulcers and some types of cancer.

Brad Pierce, an assistant professor of chemistry/biochemistry at The University of Texas at Arlington, recently led a team that examined an oxygen utilizing iron enzyme called cysteine dioxygenase or CDO, which is found in high levels within heart, liver, and brain tissues. Enzymes are proteins that act as catalysts to enable metabolic functions, but under some circumstances these oxygen-dependent enzymes can also produce highly toxic side products called reactive oxygen species or ROS.

For the first time, Pierce's team found that mutations outside the CDO active site environment or "outer coordination sphere" have a profound influence on the release of ROS. Excess ROS has been linked to numerous age-onset human disease states.

"Most research in the past has focused on the active site inner coordination sphere of these enzymes, where the metal molecule is located," said Pierce. "What we're finding is that it's really the second sphere that regulates the efficiency of the enzyme. In essence, these interactions hold everything together during catalysis. When this process breaks down, the enzyme ends up spitting out high levels of ROS and increasing the likelihood of disease."

The study was published in December by the American Chemical Society journal Biochemistry. Pierce is corresponding author on the paper, with UT Arlington students Wei Li, Michael D. Pecore and Joshua K. Crowell as co-authors. Co-author Elizabeth J. Blaesi is a graduate research assistant at the University of Wisconsin.

Pierce believes the findings from the CDO enzyme could be applied to other oxygen-dependent enzymes, which make up about 20 percent of the enzymes in the human body.

"In principle, these findings could be extended to better understand how other enzymes within the class generate ROS and potentially be used to screen for genetic dispositions for ROS-related diseases," he said.

Pierce's research brings a new level of detail to enzyme study through the use of electron paramagnetic resonance or EPR, a technology similar to the magnetic resonance imaging or MRI used in the medical field. In fall 2012, the National Science Foundation awarded Pierce a three-year, $300,000 grant to study enzymes that are catalysts for the oxidation of sulfur-bearing molecules in the body.

"Dr. Pierce's research is a good example of how basic science can set a path toward discoveries that affect human health. We look forward to his continued exploration of these findings," said Pamela Jansma, dean of the UT Arlington College of Science.


Story Source:

The above story is based on materials provided by University of Texas at Arlington. Note: Materials may be edited for content and length.


Journal Reference:

  1. Wei Li, Elizabeth J. Blaesi, Michael D. Pecore, Joshua K. Crowell, Brad S. Pierce. Second-Sphere Interactions between the C93–Y157 Cross-Link and the Substrate-Bound Fe Site Influence the O2Coupling Efficiency in Mouse Cysteine Dioxygenase. Biochemistry, 2013; 52 (51): 9104 DOI: 10.1021/bi4010232

Cite This Page:

University of Texas at Arlington. "Research may unlock enzyme's role in disease." ScienceDaily. ScienceDaily, 2 January 2014. <www.sciencedaily.com/releases/2014/01/140102165713.htm>.
University of Texas at Arlington. (2014, January 2). Research may unlock enzyme's role in disease. ScienceDaily. Retrieved April 16, 2014 from www.sciencedaily.com/releases/2014/01/140102165713.htm
University of Texas at Arlington. "Research may unlock enzyme's role in disease." ScienceDaily. www.sciencedaily.com/releases/2014/01/140102165713.htm (accessed April 16, 2014).

Share This



More Plants & Animals News

Wednesday, April 16, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Ebola Outbreak Now Linked To 121 Deaths

Ebola Outbreak Now Linked To 121 Deaths

Newsy (Apr. 15, 2014) The ebola virus outbreak in West Africa is now linked to 121 deaths. Health officials fear the virus will continue to spread in urban areas. Video provided by Newsy
Powered by NewsLook.com
In Washington, a Push to Sterilize Stray Cats

In Washington, a Push to Sterilize Stray Cats

AFP (Apr. 14, 2014) To curb the growing numbers of feral cats in the US capital, the Washington Humane Society is encouraging residents to set traps and bring the animals to a sterilization clinic, after which they are released.. Duration: 02:29 Video provided by AFP
Powered by NewsLook.com
After Attack, Officials Kill 5 Bears in Florida

After Attack, Officials Kill 5 Bears in Florida

AP (Apr. 14, 2014) Florida wildlife officials say they have killed five bears following an attack on a woman in a suburban subdivision in central Florida. Forty-five year-old Terri Frana was attacked by a large bear in her driveway Saturday. (April 14) Video provided by AP
Powered by NewsLook.com
Uruguay Opens Its First Cannabis Library

Uruguay Opens Its First Cannabis Library

AFP (Apr. 13, 2014) Uruguay opened its first Cannabis Library in Montevideo on Saturday, where people can come and read books on cannabis or take classes on how to grow the plant or even how to cook with it. Duration: 01:20 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins