Featured Research

from universities, journals, and other organizations

Improved identification of war wound infections promises more successful treatment

Date:
May 29, 2014
Source:
American Society for Microbiology
Summary:
War wounds that heal successfully frequently contain different microbial species from those that heal poorly, according to a paper. These and other findings have important implications for improving wound healing, says the first author. The investigators examined 124 wound samples from 61 wounds in 44 patients injured in combat in Iraq and Afghanistan. They used a microbial detection microarray that contains DNA probes capable of detecting any microorganisms that have previously been sequenced.

War wounds that heal successfully frequently contain different microbial species from those that heal poorly, according to a paper published ahead of print in the Journal of Clinical Microbiology. These and other findings have important implications for improving wound healing, says first author Nicholas Be of Lawrence Livermore National Laboratory, Livermore, California.

The problem the researchers were addressing is that culture-based identification, which has been used to assay war wound infections, misses the many species that are difficult or impossible to culture. But Be and his collaborators posited that using microarrays and whole genome sequencing to detect microbial species in wound samples would reveal infections caused by microbes that cannot be cultured, as these molecular methods can detect all species for which reference DNA is available.

"We also hypothesized that different microorganisms could be associated with successful or unsuccessful healing, and we felt that this information could be used for guiding medical treatment," says Be.

In the study, the investigators found that genetic sequences from certain bacteria, including Pseudomonas species and Acinetobacter baumannii, were frequently observed in wounds that failed to heal, while bacteria typically associated with the gastrointestinal system, such as E. coli and Bacteroides species, were found in wounds that did heal successfully.

"This surprising finding further emphasizes the need for specific molecular detection," says Be. "We also observed via whole genome sequencing that the complex microbial populations present in wounds vary between patients and change over time in a single patient, further emphasizing the need for personalized treatment of individual wounds."

The investigators examined 124 wound samples from 61 wounds in 44 patients injured in combat in Iraq and Afghanistan. They used a microbial detection microarray developed at Lawrence Livermore National Laboratory, which contains DNA probes capable of detecting any microorganisms that have previously been sequenced.

"This represents a cost-effective, high-throughput platform for analysis of wound infections," says Be. A subset of samples was also subjected to whole genome sequencing.

"Information on the presence of specific bacteria that more significantly affect the success of the healing response could guide therapy and allow for more accurate prediction of outcome," says Be. "More effective, specific, and timely diagnosis of infection would improve treatment, accelerate rehabilitation, and decrease the length of hospital stays."


Story Source:

The above story is based on materials provided by American Society for Microbiology. Note: Materials may be edited for content and length.


Journal Reference:

  1. N. A. Be, J. E. Allen, T. S. Brown, S. N. Gardner, K. S. McLoughlin, J. A. Forsberg, B. C. Kirkup, B. A. Chromy, P. A. Luciw, E. A. Elster, C. J. Jaing. Microbial profiling of combat wound infection through detection microarray and next-generation sequencing. Journal of Clinical Microbiology, 2014; DOI: 10.1128/JCM.00556-14

Cite This Page:

American Society for Microbiology. "Improved identification of war wound infections promises more successful treatment." ScienceDaily. ScienceDaily, 29 May 2014. <www.sciencedaily.com/releases/2014/05/140529142456.htm>.
American Society for Microbiology. (2014, May 29). Improved identification of war wound infections promises more successful treatment. ScienceDaily. Retrieved October 20, 2014 from www.sciencedaily.com/releases/2014/05/140529142456.htm
American Society for Microbiology. "Improved identification of war wound infections promises more successful treatment." ScienceDaily. www.sciencedaily.com/releases/2014/05/140529142456.htm (accessed October 20, 2014).

Share This



More Health & Medicine News

Monday, October 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Microneedle Patch Promises Painless Pricks

Microneedle Patch Promises Painless Pricks

Reuters - Innovations Video Online (Oct. 18, 2014) Researchers at The National University of Singapore have invented a new microneedle patch that could offer a faster and less painful delivery of drugs such as insulin and painkillers. Video provided by Reuters
Powered by NewsLook.com
Raw: Nurse Nina Pham Arrives in Maryland

Raw: Nurse Nina Pham Arrives in Maryland

AP (Oct. 17, 2014) The first nurse to be diagnosed with Ebola at a Dallas hospital walked down the stairs of an executive jet into an ambulance at an airport in Frederick, Maryland, on Thursday. Pham will be treated at the National Institutes of Health. (Oct. 16) Video provided by AP
Powered by NewsLook.com
Raw: Cruise Ship Returns to US Over Ebola Fears

Raw: Cruise Ship Returns to US Over Ebola Fears

AP (Oct. 17, 2014) A Caribbean cruise ship carrying a Dallas health care worker who is being monitored for signs of the Ebola virus is heading back to Texas, US, after being refused permission to dock in Cozumel, Mexico. (Oct. 17) Video provided by AP
Powered by NewsLook.com
Spanish Govt: Four Suspected Ebola Cases in Spain Test Negative

Spanish Govt: Four Suspected Ebola Cases in Spain Test Negative

AFP (Oct. 17, 2014) All four suspected Ebola cases admitted to hospitals in Spain on Thursday have tested negative for the deadly virus in a first round of tests, the government said Friday. Duration: 00:55 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins