Featured Research

from universities, journals, and other organizations

Time-lapse study reveals bottlenecks in stem cell expansion

Date:
June 12, 2014
Source:
Sheffield, University of
Summary:
Human pluripotent stems cells have the ability to produce any cell type in the body. Now, a time-lapse study of human embryonic stems cells has identified bottlenecks restricting the formation of colonies, a discovery that could improve their use in regenerative medicine. “We study pluripotent stem cells, which have huge potential for use in regenerative medicine due to their ability to become any cell in the human body. A pre-requisite for this is maintaining large numbers of undifferentiated cells in culture," noted one researcher.

A time-lapse study of human embryonic stems cells has identified bottlenecks restricting the formation of colonies, a discovery that could improve their use in regenerative medicine.

Biologists at the University of Sheffield's Centre for Stem Cell Biology led by Professor Peter Andrews and engineers in the Complex Systems and Signal Processing Group led by Professor Daniel Coca studied human pluripotent stem cells, which are a potential source of cells for regenerative medicine because they have the ability to produce any cell type in the body.

However, using these stem cells in therapies is currently hampered by the fact they can acquire genetic changes during prolonged culture which are non-random and resemble mutations in cancer cells.

Researchers used time-lapse imaging of single human embryonic stem cells to identify aspects of their behaviour that restrict growth and would be targets for mutations that allow cells to grow more efficiently.

Dr Ivana Barbaric, from the University of Sheffield's Department of Biomedical Science, said: "We study pluripotent stem cells, which have huge potential for use in regenerative medicine due to their ability to become any cell in the human body. A pre-requisite for this is maintaining large numbers of undifferentiated cells in culture.

"However, there are several obstacles such as cells tend to die extensively during culturing and they can mutate spontaneously. Some of these genetic mutations are known to provide stem cells with superior growth, allowing them to overtake the culture -- a phenomenon termed culture adaptation, which mimics the behaviour of cancer cells.

"In order for pluripotent stem cells to be used safely in regenerative medicine we need to understand how suboptimal culture conditions, for example culturing cells at low split ratios, affect the cells and can lead to culture adaptation."

The team's research combined the use of time-lapse microscopy, single-cell tracking and mathematical modelling to characterise bottlenecks affecting the survival of normal human embryonic stem cells and compared them with adapted cells.

They identified three major bottlenecks affecting colony formation: survival after plating, failure to re-enter into cell cycle and continued cell death after division.

In the same culture condition, they found adapted cells performed better in all of these points leading to more colonies. Bottlenecks were also alleviated through cell to cell contact and pro-survival compounds.

Dr Veronica Biga, from the University's Automatic Control and Systems Engineering Department, said: "To extract information about cell death, mitosis and movement, we developed new methods for analysing images and measuring numerous parameters from time-lapse videos."

She added: "We plan to further develop the methods from this study into an image processing and analysis software solution to be used for monitoring cell behaviour in applications such as screening culture conditions, drug discovery, monitoring and minimising the occurrence of genetic abnormalities directly through time-lapse."


Story Source:

The above story is based on materials provided by Sheffield, University of. Note: Materials may be edited for content and length.


Journal Reference:

  1. Ivana Barbaric, Veronica Biga, PaulJ. Gokhale, Mark Jones, Dylan Stavish, Adam Glen, Daniel Coca, PeterW. Andrews. Time-Lapse Analysis of Human Embryonic Stem Cells Reveals Multiple Bottlenecks Restricting Colony Formation and Their Relief upon Culture Adaptation. Stem Cell Reports, 2014; DOI: 10.1016/j.stemcr.2014.05.006

Cite This Page:

Sheffield, University of. "Time-lapse study reveals bottlenecks in stem cell expansion." ScienceDaily. ScienceDaily, 12 June 2014. <www.sciencedaily.com/releases/2014/06/140612121320.htm>.
Sheffield, University of. (2014, June 12). Time-lapse study reveals bottlenecks in stem cell expansion. ScienceDaily. Retrieved October 22, 2014 from www.sciencedaily.com/releases/2014/06/140612121320.htm
Sheffield, University of. "Time-lapse study reveals bottlenecks in stem cell expansion." ScienceDaily. www.sciencedaily.com/releases/2014/06/140612121320.htm (accessed October 22, 2014).

Share This



More Health & Medicine News

Wednesday, October 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Orthodontist Mom Jennifer Salzer on the Best Time for Braces

Orthodontist Mom Jennifer Salzer on the Best Time for Braces

Working Mother (Oct. 22, 2014) Is your child ready? Video provided by Working Mother
Powered by NewsLook.com
U.S. Issues Ebola Travel Restrictions, Are Visa Bans Next?

U.S. Issues Ebola Travel Restrictions, Are Visa Bans Next?

Newsy (Oct. 22, 2014) Now that the U.S. is restricting travel from West Africa, some are dropping questions about a travel ban and instead asking about visa bans. Video provided by Newsy
Powered by NewsLook.com
US to Track Everyone Coming from Ebola Nations

US to Track Everyone Coming from Ebola Nations

AP (Oct. 22, 2014) Stepping up their vigilance against Ebola, federal authorities said Wednesday that everyone traveling into the US from Ebola-stricken nations will be monitored for symptoms for 21 days. (Oct. 22) Video provided by AP
Powered by NewsLook.com
Doctors Help Paralysed Man Walk Again, Patient in Disbelief

Doctors Help Paralysed Man Walk Again, Patient in Disbelief

AFP (Oct. 22, 2014) Polish doctors describe how they helped a paralysed man walk again, with the patient in disbelief at the return of sensation to his legs. Duration: 1:04 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins