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Beware of microbial traffic jams

Biophysicist finds that rapidly proliferating yeast pack quite a punch: Up to 150 psi

Date:
May 13, 2016
Source:
University of California - Berkeley
Summary:
Grains of sand in an hourglass or candy in a gumball machine often jam up, and physicists have studied this granular flow for decades.Biophysicists have now found that multiplying yeast cells can jam up too, and be described by some of the same physical and mathematical principles. The jammed yeast can actually exert strong pressure that microbes may use to alter their environment.
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A wild strain of yeast growing in a tiny chamber gradually fills the space and, because the yeast cells are so sticky, easily clog the chamber and explode out of it.
Credit: Image courtesy of University of California - Berkeley

When tiny microbes jam up like fans exiting a baseball stadium, they can do some real damage.

University of California, Berkeley, physicists found this out the hard way when the baker's yeast cells (Saccharomyces cerevisiae) they were studying multiplied so prolifically that they burst the tiny chamber in which they were being raised.

When UC Berkeley postdoctoral fellow Morgan Delarue measured the force the growing mass of cells exerted as they pushed against one another, he calculated that it can be nearly five times higher than the pressure in a car tire -- about 150 psi, or 10 times atmospheric pressure.

This is more than just a weird observation, said Oskar Hallatschek, a UC Berkeley assistant professor of physics and leader of the team. Budding yeast or other living cells, which split in two and grow exponentially in number, may well generate such mechanical forces to alter their environment, possibly in damaging ways. This may be even more important for cells like yeast that cannot move.

"Our results suggest that self-driven jamming and the build up of large pressures is a natural tendency of proliferating cells, and may be contributing to microbial pathogenesis and biofouling," he said. Biofouling is when bacteria or other organisms grow so prolifically as to interfere with the operation of machinery, such as happens in water pumps.

In fact, when graduate student Jörn Hartung grew yeast in a gel, he found that they split the gel, a possible example of how they could create cracks in rocks or soil particles.

"The mechanism that allows these populations of cells to generate such forces could be relevant to remodeling the microenvironment," Hallatschek said. "If you are constrained, maybe it's good to be able to break the material and change the pore's sizes in the environment."

Hallatschek, Delarue, Hartung and their colleagues at UC Berkeley and the Max Planck Institute for Dynamics and Self-Organization in Göttingen, Germany, reported their findings this week in the journal Nature Physics.

In a commentary in the same issue of the journal, physicists Shreyas Gokhale and Jeff Gore of the Massachusetts Institute of Technology wrote that the work may "prove to be the starting point for a new class of experiments at the interface of physics and biology."

Microbial avalanches

Hallatschek was exploring the consequences of space limitations on the biophysics of microbes, and developed a microfluidic bioreactor 30 microns across -- about one ten thousandth of an inch -- in which he could grow yeast cells and control the degree of confinement.

His experiments showed that when microbes grow in narrow channels, they tend to get stuck and unstuck like sand grains flowing down a hopper or candy from a gumball machine. The result is an intermittent flow characterized by stoppages and avalanches.

"People have looked at sand flowing down a hopper and observe a very jerky flow like we've observed here," he said. "This is due to the formation of force chains that form a bridge that holds back the sand flow for a while. When one of the bridges breaks, the sand flows again. So you get a series of stop-and-go avalanches."

As a consequence of this self-inflicted jamming, microbes can generate mechanical contact pressures of up to 1 megapascal: large enough to strain or even crack the confining cavity.

Hallatschek's team is currently simulating the yeast cell growth computationally, using flowing and "proliferating" sand as his model.

Video: https://www.youtube.com/watch?v=SQ_rVZz8QLQ


Story Source:

Materials provided by University of California - Berkeley. Original written by Robert Sanders. Note: Content may be edited for style and length.


Journal References:

  1. Morgan Delarue, Jörn Hartung, Carl Schreck, Pawel Gniewek, Lucy Hu, Stephan Herminghaus, Oskar Hallatschek. Self-driven jamming in growing microbial populations. Nature Physics, 2016; DOI: 10.1038/nphys3741
  2. Shreyas Gokhale, Jeff Gore. Biophysics: Life in a jam. Nature Physics, 2016; DOI: 10.1038/nphys3777

Cite This Page:

University of California - Berkeley. "Beware of microbial traffic jams: Biophysicist finds that rapidly proliferating yeast pack quite a punch: Up to 150 psi." ScienceDaily. ScienceDaily, 13 May 2016. <www.sciencedaily.com/releases/2016/05/160513130239.htm>.
University of California - Berkeley. (2016, May 13). Beware of microbial traffic jams: Biophysicist finds that rapidly proliferating yeast pack quite a punch: Up to 150 psi. ScienceDaily. Retrieved May 26, 2017 from www.sciencedaily.com/releases/2016/05/160513130239.htm
University of California - Berkeley. "Beware of microbial traffic jams: Biophysicist finds that rapidly proliferating yeast pack quite a punch: Up to 150 psi." ScienceDaily. www.sciencedaily.com/releases/2016/05/160513130239.htm (accessed May 26, 2017).

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