Featured Research

from universities, journals, and other organizations

New Form Of Gene Therapy Holds Promise For The Future

Date:
October 25, 1997
Source:
University Of Texas Southwestern Medical Center At Dallas
Summary:
Scientists at UT Southwestern Medical Center at Dallas are one step closer to producing a "drug" that is internally regulated and activated only when needed.

DALLAS - October 23, 1997 - Scientists at UT Southwestern Medical Center at Dallas are one step closer to producing a "drug" that is internally regulated and activated only when needed.

They have developed a system in mice in which the level of a genetically engineered protein responds to inflammatory signals produced by the mice themselves. This method of gene therapy, described in the October issue of Nature Biotechnology, may have great potential for treating chronic relapsing and remitting inflammatory diseases, such as rheumatoid arthritis, and organ transplant rejection.

"Our long-range goal is to give patients the right amount of an anti-inflammatory protein, at the right time and in the right place, to control damaging inflammation by introducing the gene for the protein and allowing the body's own signals to control its production," said Dr. Robert Munford, professor of internal medicine and microbiology and holder of the Jan and Henri Bromberg Chair in Internal Medicine.

According to Munford, who worked with Dr. Alan Varley, a research fellow in internal medicine, and research technician Steven Geiszler, "There are lots of hurdles to overcome, but Varley and Geiszler seem to have jumped the first one, showing that recombinant genes can actually be regulated in animals in response to inflammation."

The investigators used a "reporter" gene - a gene that encodes an easily measured protein - to test the ability of a mouse's immune response to turn on that gene. The reporter gene they used was firefly luciferase, an enzyme that causes light emission and can be measured easily with a luminometer. In the laboratory, they inserted the luciferase gene into a genetically altered virus that could not reproduce. To stimulate and control the production of luciferase, the researchers inserted specific short pieces of deoxyribonucleic acid (DNA) in front of the luciferase gene. These short DNA elements respond to internal signals by turning genes on and off.

The trick was to find the right combination of DNA elements to dramatically enhance production of luciferase in response to an inflammatory reaction.

The successful combination consisted of three elements ? one from the mouse and two from a virus ? that worked in concert and greatly amplified the production of luciferase when the proper signals (in this case, an inflammatory reaction) were received.

The luciferase gene preceded by the three short pieces of DNA was genetically inserted into the viral molecule and injected into mice. Researchers then induced two different types of inflammatory responses. They determined how successful their combination of elements was by measuring the amount of luciferase produced in the mouse's liver, spleen, lung, heart and kidney.

If a gene for an anti-inflammatory protein is used in place of the luciferase gene, this type of gene therapy, in theory, would activate that protein in response to the body's own inflammatory signals.

"The production level of the anti-inflammatory protein should reflect the intensity and duration of the inflammatory condition," said Munford. "If the gene can be delivered to a specific site, such as an inflamed joint or an organ about to be transplanted into a recipient, it may be possible to provide effective anti-inflammatory treatment while avoiding systemic immunosuppression with its risk of infection."

Dr. Richard Gaynor, professor of internal medicine and microbiology, and holder of the Andrea L. Simmons Distinguished Chair in Cancer Virology, also collaborated in the studies.


Story Source:

The above story is based on materials provided by University Of Texas Southwestern Medical Center At Dallas. Note: Materials may be edited for content and length.


Cite This Page:

University Of Texas Southwestern Medical Center At Dallas. "New Form Of Gene Therapy Holds Promise For The Future." ScienceDaily. ScienceDaily, 25 October 1997. <www.sciencedaily.com/releases/1997/10/971025092355.htm>.
University Of Texas Southwestern Medical Center At Dallas. (1997, October 25). New Form Of Gene Therapy Holds Promise For The Future. ScienceDaily. Retrieved July 30, 2014 from www.sciencedaily.com/releases/1997/10/971025092355.htm
University Of Texas Southwestern Medical Center At Dallas. "New Form Of Gene Therapy Holds Promise For The Future." ScienceDaily. www.sciencedaily.com/releases/1997/10/971025092355.htm (accessed July 30, 2014).

Share This




More Health & Medicine News

Wednesday, July 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Health Insurers' Profits Slide

Health Insurers' Profits Slide

Reuters - Business Video Online (July 30, 2014) Obamacare-related costs were said to be behind the profit plunge at Wellpoint and Humana, but Wellpoint sees the new exchanges boosting its earnings for the full year. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com
Concern Grows Over Worsening Ebola Crisis

Concern Grows Over Worsening Ebola Crisis

AFP (July 30, 2014) Pan-African airline ASKY has suspended all flights to and from the capitals of Liberia and Sierra Leone amid the worsening Ebola health crisis, which has so far caused 672 deaths in Guinea, Liberia and Sierra Leone. Duration: 00:43 Video provided by AFP
Powered by NewsLook.com
At Least 20 Chikungunya Cases in New Jersey

At Least 20 Chikungunya Cases in New Jersey

AP (July 30, 2014) At least 20 New Jersey residents have tested positive for chikungunya, a mosquito-borne virus that has spread through the Caribbean. (July 30) Video provided by AP
Powered by NewsLook.com
Xtreme Eating: Your Daily Caloric Intake All On One Plate

Xtreme Eating: Your Daily Caloric Intake All On One Plate

Newsy (July 30, 2014) The Center for Science in the Public Interest released its 2014 list of single meals with whopping calorie counts. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



      Save/Print:
      Share:

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile: iPhone Android Web
      Follow: Facebook Twitter Google+
      Subscribe: RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins