Featured Research

from universities, journals, and other organizations

Purdue Study: RNA 'Motor' Transports DNA In Virus

Date:
July 27, 1998
Source:
Purdue University
Summary:
Long known as messengers, transcribers and translators for DNA, the hard-working family of RNA molecules may also serve as chauffeurs.

WEST LAFAYETTE, Ind. -- Long known as messengers, transcribers and translators for DNA, the hard-working family of RNA molecules may also serve as chauffeurs.Peixuan Guo, professor of molecular virology at Purdue University, has found that a virus known as Bacteriophage Phi 29 uses six RNAs strung together in the shape of a hexagon to create a motor that transports DNA in the virus.

Related Articles


His findings were published in the July issue of the scientific journal Molecular Cell . A review covering this study also appears in the July 24 issue of Cell .

Guo's findings represent the first example of a hexagonal-shaped RNA complex. It is also the first example of transportation vehicles using RNA as building blocks.

Information from the study may someday be used to develop nanoscale devices and will also improve scientists' understanding of how cells transport large molecules through barriers such as membranes.

In the study, Guo's lab found that Bacteriophage Phi 29, a virus that infects one type of bacteria, uses six RNA to form a hexagon that acts in a manner similar to a six-cylinder car engine to drive DNA into the outer shell of the virus.

Deoxyribonucleic acid, or DNA, and ribonucleic acid, RNA, are biological molecules that allow organisms to reproduce. While DNA holds the genetic material, RNA carries out a number of functions, including the synthesis of proteins specified by DNA.

Guo says that Bacteriophage Phi 29 is typical of double-stranded DNA viruses in that its genetic material is packaged into its protein shell, or capsid, during maturation.

"All linear double-stranded DNA viruses, including herpes viruses, adenoviruses, pox viruses and the double-stranded DNA bacteriophages, package their genomic DNA into a pre-formed protein shell," he says. "What makes Phi 29 unique is that it is the first virus to be reported to use RNA as a component of the transportation machine to drive this process."

Moving large molecules, such as DNA and RNA, through barriers such as cellular membranes is a common process in biological systems. But the migration mechanisms used to package viral DNA within its outer shell are among the most complex and intricate of such processes, Guo says.

In 1987, Guo discovered this "transporting" RNA species and provided the first evidence that RNA played a role in packaging DNA in Phi 29. This new type of RNA was dubbed "pRNA" for "packaging" RNA. Subsequent reports by Guo and others have since established the presence and molecular structure of pRNA.

In his latest study, Guo shows how the structure of pRNA allows the molecules to interlock in hexagon formation, and how the hexagon functions to drive DNA through its portal in a manner similar to a bolt and a screw.

"The RNA hexagon is like a bolt that is embedded in the five-sided shell of the virus, and DNA is helical, like a screw," Guo says. "Because DNA is helical, like a screw, we envision it twisting and turning through the RNA portal, and such a five-fold/six-fold mismatch would facilitate the rotation."

Guo speculates that the force that drives this action likely comes from the contraction and relaxation of the six RNA molecules, which "fire" in a sequential manner, similar to a six-cylinder car engine.

"Some evidence for such a theory has been reported," he says.

Instead of gasoline, the DNA packaging machine uses ATP, a compound vital to producing energy processes in all living cells, to provide energy for its motor, Guo says.

"ATP provides energy to condense the lengthy DNA into the limited space inside procapsids with remarkable velocity," he says.

Though Phi 29 is the first virus reported to use RNA as a motor, other related bacterial viruses have been reported to contain pRNA, says Guo. In addition, he speculates that some RNAs may perform a similar function in animal or human cells.

Guo's studies at Purdue are funded by the National Institutes of Health and the National Science Foundation.

Web Sites: Peixuan Guo, http://biochem.purdue.edu/~bmb/faculty/quo.html

Molecular Cell, http://www.molecule.org/current.shtml

NOTE TO JOURNALISTS: For copies of the journal articles mentioned in the story, contact Susan Gaidos at Purdue News Service, (765) 494-2081.

ABSTRACT

Inter-RNA Interaction of Phage Phi 29 pRNA to Form a Hexameric Complex for Viral DNA Transportation Peixuan Guo*, Chunlin Zhang, Chaoping Chen, Chunlin Zhang, Kyle Garver and Mark Trottier

Ds-DNA viruses package their DNA into a pre-formed protein shell (procapsid) during maturation. Bacteriophage 29 requires an RNA (pRNA) to package it genomic DNA into the procapsid. We report here that the pRNA upper and lower loops are involved in RNA/RNA interactions. Mutation in only one loop results in inactive pRNAs. However, mixing of two, three and six inactive mutant pRNAs restores DNA packaging activity as long as an interlocking hexameric ring can be predicted to form by base pairing of the mutated loops in separate RNA molecules. The stoichiometry of pRNA for the packaging of one viral DNA genome is six. Homogeneous pRNA purified from a single band in denaturing gels showed six bands when rerun in native gels. These results suggest that six pRNAs form a hexameric ring by the intermolecular interaction of two RNA loops to serve as part of the DNA transportation machinery.


Story Source:

The above story is based on materials provided by Purdue University. Note: Materials may be edited for content and length.


Cite This Page:

Purdue University. "Purdue Study: RNA 'Motor' Transports DNA In Virus." ScienceDaily. ScienceDaily, 27 July 1998. <www.sciencedaily.com/releases/1998/07/980727080433.htm>.
Purdue University. (1998, July 27). Purdue Study: RNA 'Motor' Transports DNA In Virus. ScienceDaily. Retrieved April 25, 2015 from www.sciencedaily.com/releases/1998/07/980727080433.htm
Purdue University. "Purdue Study: RNA 'Motor' Transports DNA In Virus." ScienceDaily. www.sciencedaily.com/releases/1998/07/980727080433.htm (accessed April 25, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Saturday, April 25, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

85 Killed in Niger by Meningitis Since Start of Year

85 Killed in Niger by Meningitis Since Start of Year

AFP (Apr. 24, 2015) A meningitis outbreak in Niger has killed 85 people since the start of the year prompting authorities to close schools in the capital Niamey until Monday. Video provided by AFP
Powered by NewsLook.com
C-Section Births a Trend in Brazil

C-Section Births a Trend in Brazil

AFP (Apr. 24, 2015) More than half of Brazil&apos;s babies are born via cesarean section, as mothers and doctors opt for a faster and less painful experience despite the health risks. Duration: 02:02 Video provided by AFP
Powered by NewsLook.com
Anti-Malaria Jab Hope

Anti-Malaria Jab Hope

Reuters - News Video Online (Apr. 24, 2015) The world&apos;s first anti-malaria vaccine could get the go-ahead for use in Africa from October if approved by international regulators. Paul Chapman reports. Video provided by Reuters
Powered by NewsLook.com
3D Food Printing: The Meal of the Future?

3D Food Printing: The Meal of the Future?

AP (Apr. 23, 2015) Developers of 3D food printing hope the culinary technology will revolutionize the way we cook and eat. (April 23) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins