Featured Research

from universities, journals, and other organizations

Researchers Discover Fast, Efficient, Controllable Way To Study Molecular Interactions

Date:
May 25, 1999
Source:
University Of Texas Southwestern Medical Center
Summary:
A molecule that is extremely sensitive to light has proved a highly efficient way of initiating bonding of two proteins. UT Southwestern Medical Center at Dallas researchers who designed this cross-linking reagent and technique said this will be an important tool in studying multiprotein complexes and may also help with drug development.

DALLAS - May 25, 1999 - A molecule that is extremely sensitive to light has proved a highly efficient way of initiating bonding of two proteins. UT Southwestern Medical Center at Dallas researchers who designed this cross-linking reagent and technique said this will be an important tool in studying multiprotein complexes and may also help with drug development.

The study, in today's issue of The Proceedings of the National Academy of Sciences, reports the first protein cross-linking reagent that is activated by brief exposure to visible light, allowing observation of relationships between molecules in a time-controlled fashion.

"Our goal is to develop new methods to probe interaction between proteins in their native environment," said Dr. Thomas Kodadek, professor of internal medicine and biochemistry and lead author of the paper. "One problem with traditional methods of chemical cross-linking is lack of control over when the chemical reaction takes place. We wanted to invent something that was inert until you trigger it."

The investigators exposed ruthenium (II) tris-bipyridyl dication Ru(II)bpy32+ in the presence of ammonium persulfate and interacting proteins to light from a high-intensity 150W lamp. They found that in only .5 seconds 60 percent of the molecules were coupled. Similar results occurred using a standard flashlight by just increasing the exposure time to five to 30 seconds and holding the sample closer to the light. Thus, no specialized equipment is necessary to use this technique.

The technique, dubbed photo-induced cross-linking of unmodified proteins, is about 1,000 times more efficient than any previously used chemical methods.

The researchers picked Ru(II) because it absorbs light very efficiently and turns the energy into chemical reactions. Since this is a nonperturbing, light-triggered process, it doesn't corrupt concurrent biochemical processes. Using light as the activator also allows control over exactly when the bonding takes place.

Eventually, the scientists believe that this will aid in drug creation.

"Right now this is core technology for studying protein interactions," said Kodadek, a principal investigator for the Center for Biomedical Inventions at UT Southwestern. "The technological spinoff will be when we can use this cross-linking technique to determine a drug target within a cell."

The other researcher on this study was Dr. David Fancy, postdoctoral fellow in pharmacology and biochemistry.

A National Institutes of Health grant funded the study.


Story Source:

The above story is based on materials provided by University Of Texas Southwestern Medical Center. Note: Materials may be edited for content and length.


Cite This Page:

University Of Texas Southwestern Medical Center. "Researchers Discover Fast, Efficient, Controllable Way To Study Molecular Interactions." ScienceDaily. ScienceDaily, 25 May 1999. <www.sciencedaily.com/releases/1999/05/990525061614.htm>.
University Of Texas Southwestern Medical Center. (1999, May 25). Researchers Discover Fast, Efficient, Controllable Way To Study Molecular Interactions. ScienceDaily. Retrieved August 21, 2014 from www.sciencedaily.com/releases/1999/05/990525061614.htm
University Of Texas Southwestern Medical Center. "Researchers Discover Fast, Efficient, Controllable Way To Study Molecular Interactions." ScienceDaily. www.sciencedaily.com/releases/1999/05/990525061614.htm (accessed August 21, 2014).

Share This




More Health & Medicine News

Thursday, August 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Possible Ebola Patient in Isolation at California Hospital

Possible Ebola Patient in Isolation at California Hospital

Reuters - US Online Video (Aug. 20, 2014) — A patient who may have been exposed to the Ebola virus is in isolation at the Kaiser Permanente South Sacramento Medical Center. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Raw: World's Oldest Man Lives in Japan

Raw: World's Oldest Man Lives in Japan

AP (Aug. 20, 2014) — A 111-year-old Japanese was certified as the world's oldest man by Guinness World Records on Wednesday. Sakari Momoi, a native of Fukushima in northern Japan, was given a certificate at a hospital in Tokyo. (Aug. 20) Video provided by AP
Powered by NewsLook.com
Do More Wedding Guests Make A Happier Marriage?

Do More Wedding Guests Make A Happier Marriage?

Newsy (Aug. 20, 2014) — A new study found couples who had at least 150 guests at their weddings were more likely to report being happy in their marriages. Video provided by Newsy
Powered by NewsLook.com
Ebola-Hit Sierra Leone's Freetown a City on Edge

Ebola-Hit Sierra Leone's Freetown a City on Edge

AFP (Aug. 19, 2014) — Residents of Sierra Leone's capital voice their fears as the Ebola virus sweeps through west Africa. Duration: 00:56 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins