Featured Research

from universities, journals, and other organizations

Gene Therapy Reverses Heart Disease In Mice

Date:
February 11, 2000
Source:
American Heart Association
Summary:
A new gene therapy tested in mice all but eliminated the fatty plaque deposits that can build up in arteries and cause heart attacks and strokes, according to a study in the February issue of Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association.

DALLAS, Feb. 11 -- A new gene therapy tested in mice all but eliminated the fatty plaque deposits that can build up in arteries and cause heart attacks and strokes, according to a study in the February issue of Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association. Researchers transplanted a gene for the human version of apolipoprotein E (apoE) into a strain of mice bred specifically for the experiment. ApoE is a key protein involved in transporting and clearing cholesterol from the body. The animals getting the apoE gene had significant reductions in total cholesterol and triglycerides, an increased level of beneficial high-density lipoprotein (HDL) cholesterol, and complete regression of the fatty plaque blockages.

"There were tremendous differences in the number of fatty deposits between our treated and untreated animals, and we can say there were almost zero of the fatty deposits in the blood vessels of the gene therapy group," says senior author Nicolas Duverger, Ph.D., of Gencell in Vitry/Seine, France, a division of the pharmaceutical company Rhone-Poulenc Rorer. "Our first thought was to see if we could stop plaque development. But in fact, we observed complete regression of the lesions," he says.

The research was conducted in collaboration with scientists at the Pasteur Institute in Paris and Lille, France.

"There is a medical need for effective new therapies to treat people with high cholesterol levels," Duverger says. Eventually scientists may devise an apoE gene therapy to protect against heart disease that can be administered by injection every couple of years.

The most stunning finding occurred at about 200 days after treatment -- plaques in the gene-injected mice had essentially disappeared. "It has never been demonstrated that we could completely remove plaque in an animal or human just by altering the lipoprotein contents of the blood by a gene," Duverger says.

The researchers bred a strain of mice that had very high cholesterol levels and deficient immune systems. One group of mice received the apoE gene therapy and a second group did not.

Total cholesterol levels sank rapidly in the treated mice, dropping from a whopping level of 591 milligrams per deciliter down to just 92 mg/dL in three weeks time.

The beneficial changes in the blood cholesterol levels of the treated mice became evident on day four of the experiment. The changes persisted for at least five months in the mice getting the higher dose of the apoE gene, and then a rise in cholesterol began. Mice injected with a lower dose of the gene had a similar drop in cholesterol levels, however their levels started to rise sooner than the mice who received higher doses of the gene therapy. Researchers say it signals that the amount of improvement in cholesterol levels was dependent on the size of the dose of gene therapy.

Treated mice also had a sharp increase in HDL "good" cholesterol, which clears fat from the body, and a drop in their levels of "bad" LDL cholesterol, compared to the untreated mice. The treated mice also experienced drops in their levels of triglycerides.

ApoE sits on the surface of fat particles that circulate in the blood, including cholesterol and triglycerides. It binds to cells in the liver, which can then rid the body of the fats. High blood cholesterol is a major risk factor for coronary heart disease.

"The apoE gene binds with the particles of LDL cholesterol and triglycerides and carries them out of the body," Duverger says. However, he adds that the increased levels of HDL cholesterol are harder to explain. "It may be due to apoE binding to the HDL particles and helping to remove the plaques."

###

Co-authors are Caroline Desurmont, Ph.D.; Jean-Michel Caillaud, M.D.; Florence Emmanuel, M.D.; Patrick Benoit, M.D.; Jean Charles Fruchart, M.D.; Graciela Castro, M.D.; Didier Branellec, M.D.; and Jean-Michel Heard, M.D.


Story Source:

The above story is based on materials provided by American Heart Association. Note: Materials may be edited for content and length.


Cite This Page:

American Heart Association. "Gene Therapy Reverses Heart Disease In Mice." ScienceDaily. ScienceDaily, 11 February 2000. <www.sciencedaily.com/releases/2000/02/000211082913.htm>.
American Heart Association. (2000, February 11). Gene Therapy Reverses Heart Disease In Mice. ScienceDaily. Retrieved August 22, 2014 from www.sciencedaily.com/releases/2000/02/000211082913.htm
American Heart Association. "Gene Therapy Reverses Heart Disease In Mice." ScienceDaily. www.sciencedaily.com/releases/2000/02/000211082913.htm (accessed August 22, 2014).

Share This




More Health & Medicine News

Friday, August 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Drug Used To Treat 'Ebola's Cousin' Shows Promise

Drug Used To Treat 'Ebola's Cousin' Shows Promise

Newsy (Aug. 21, 2014) An experimental drug used to treat Marburg virus in rhesus monkeys could give new insight into a similar treatment for Ebola. Video provided by Newsy
Powered by NewsLook.com
Two US Ebola Patients Leave Hospital Free of the Disease

Two US Ebola Patients Leave Hospital Free of the Disease

AFP (Aug. 21, 2014) Two American missionaries who were sickened with Ebola while working in Liberia and were treated with an experimental drug are doing better and have left the hospital, doctors say on August 21, 2014. Duration: 01:05 Video provided by AFP
Powered by NewsLook.com
Cadavers, a Teen, and a Medical School Dream

Cadavers, a Teen, and a Medical School Dream

AP (Aug. 21, 2014) Contains graphic content. He's only 17. But Johntrell Bowles has wanted to be a doctor from a young age, despite the odds against him. He was recently the youngest participant in a cadaver program at the Indiana University NW medical school. (Aug. 21) Video provided by AP
Powered by NewsLook.com
American Ebola Patients Released: What Cured Them?

American Ebola Patients Released: What Cured Them?

Newsy (Aug. 21, 2014) It's unclear whether the American Ebola patients' recoveries can be attributed to an experimental drug or early detection and good medical care. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins