Featured Research

from universities, journals, and other organizations

Scientists Report First Transgenic Animal Developed Via Retroviral DNA Insertion Into Male Germ-Line Stem Cells

Date:
October 23, 2001
Source:
University Of Pennsylvania
Summary:
Scientists at the University of Pennsylvania School of Veterinary Medicine have successfully used a retrovirus to modify genes in spermatogonial stem cells in a mouse – the first instance, in any species, of a transgenic animal created by inserting a gene into male germ-line stem cells.

PHILADELPHIA – Scientists at the University of Pennsylvania School of Veterinary Medicine have successfully used a retrovirus to modify genes in spermatogonial stem cells in a mouse – the first instance, in any species, of a transgenic animal created by inserting a gene into male germ-line stem cells.

The inserted gene subsequently appeared in approximately 4.5 percent of offspring of mice transplanted with the altered stem cells, and was transmitted to at least three succeeding generations.

The work is the cover story in the Nov. 6 issue of the Proceedings of the National Academy of Sciences, and will appear tomorrow on the journal’s web site. The findings should enable the creation of transgenic individuals in a wide range of species, permitting scientists to develop research models for study of numerous human diseases.

Led by Ralph L. Brinster of Penn’s Department of Animal Biology, the scientists succeeded in inserting a foreign gene – in this case the common reporter gene lacZ, whose product is the enzyme ί-galactosidase – into 2 to 20 percent of mouse spermatogonial stem cells in laboratory experiments, a ten-fold improvement over previous attempts.

"These results indicate that there is no intrinsic barrier to the genetic engineering of spermatogonial stem cells using retroviruses, and that once inserted, the foreign genes will continue to be transmitted and expressed from one generation to the next," said Brinster, the Richard King Mellon Professor of Reproductive Physiology at Penn.

All male mammals harbor many spermatogonial stem cells, key repositories of genetic material whose daughter cells give rise after puberty to sperm. In the human male, approximately 1,000 sperm cells, each carrying a different combination of genetic material, are generated in this manner with each heartbeat. Whereas the female germ cell, the egg, stops dividing before birth, male germ-line stem cells continue to divide throughout life.

Of the various types of stem cells, only two – spermatogonial and hematopoeitic stem cells – can be positively identified using functional assays. This makes them valuable models for other types of stem cells, such as those that give rise to skin, the lining of the intestines, brain, muscle and liver.

Spermatogonial stem cells are of additional interest for transgenics applications because they are the only cells, including all other stem cells, that undergo self-renewal throughout an animal’s lifetime and contribute genes to subsequent generations. Previous attempts to genetically alter this unique type of stem cell, either through retroviruses or other methods, have met with little success.

Brinster’s work is unusual in that his transgenic mice were created using cells from male animals. Transgenic animals are generally produced by inserting foreign DNA into cells derived from females, such as oocytes, eggs and blastocysts. Brinster’s group has demonstrated that the efficiency of generating transgenic mice through spermatogonial stem cells is roughly equivalent to that of female-derived cells.

"Questions had been raised regarding whether male germ-line stem cells could be transduced with a retroviral vector and whether any gene introduced would be silenced," Brinster said. "Our work clearly demonstrates that the stem cell can be transduced at relatively high efficiency and expression is not silenced. About 10 percent of stem cells carry active genes that are transmitted for at least three generations."

Retroviruses are the most common vehicles for introducing genes in human somatic cell gene therapy, and some scientists had expressed concern that this approach might result in genetic alterations to germ-line cells. Brinster’s paper indicates that the germ cells are indeed susceptible to insertion of foreign genes via retroviruses, although the somatic cells that surround stem cells in the body most likely provide a protective shield.

Brinster was joined in the work by Makoto Nagano, now at McGill University, and Clayton J. Brinster, Kyle E. Orwig, Buom-Yong Ryu and Mary R. Avarbock, all of the Department of Animal Biology in Penn’s School of Veterinary Medicine. Their work was supported by the National Institutes of Health, the Commonwealth and General Assembly of Pennsylvania and the Robert J. Kleberg Jr. and Helen C. Kleberg Foundation.


Story Source:

The above story is based on materials provided by University Of Pennsylvania. Note: Materials may be edited for content and length.


Cite This Page:

University Of Pennsylvania. "Scientists Report First Transgenic Animal Developed Via Retroviral DNA Insertion Into Male Germ-Line Stem Cells." ScienceDaily. ScienceDaily, 23 October 2001. <www.sciencedaily.com/releases/2001/10/011023072036.htm>.
University Of Pennsylvania. (2001, October 23). Scientists Report First Transgenic Animal Developed Via Retroviral DNA Insertion Into Male Germ-Line Stem Cells. ScienceDaily. Retrieved September 22, 2014 from www.sciencedaily.com/releases/2001/10/011023072036.htm
University Of Pennsylvania. "Scientists Report First Transgenic Animal Developed Via Retroviral DNA Insertion Into Male Germ-Line Stem Cells." ScienceDaily. www.sciencedaily.com/releases/2001/10/011023072036.htm (accessed September 22, 2014).

Share This



More Plants & Animals News

Monday, September 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Cat Lovers Flock to Los Angeles

Cat Lovers Flock to Los Angeles

AFP (Sep. 22, 2014) — The best funny internet cat videos are honoured at LA's Feline Film Festival. Duration: 00:56 Video provided by AFP
Powered by NewsLook.com
Washed-Up 'Alien Hairballs' Are Actually Algae

Washed-Up 'Alien Hairballs' Are Actually Algae

Newsy (Sep. 22, 2014) — Green balls of algae washed up on Sydney, Australia's Dee Why Beach. Video provided by Newsy
Powered by NewsLook.com
Raw: San Diego Zoo Welcomes Cheetah Cubs

Raw: San Diego Zoo Welcomes Cheetah Cubs

AP (Sep. 20, 2014) — The San Diego Zoo has welcomed two Cheetah cubs to its Safari Park. The nearly three-week-old female cubs are being hand fed and are receiving around the clock care. (Sept. 20) Video provided by AP
Powered by NewsLook.com
Chocolate Museum Opens in Brussels

Chocolate Museum Opens in Brussels

AFP (Sep. 19, 2014) — Considered a "national heritage" in Belgium, chocolate now has a new museum in Brussels. In a former chocolate factory, visitors to the permanent exhibition spaces, workshops and tastings can discover derivatives of the cocoa bean. Duration: 01:00 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins