Featured Research

from universities, journals, and other organizations

Physical Map Of Mouse Genome Now Available

Date:
August 7, 2002
Source:
Washington University School Of Medicine
Summary:
A physical map of the genetic makeup of a mouse--the mouse genome--is 98 percent complete and is being released online by the journal Nature. Researchers at the Genome Sequencing Center at Washington University School of Medicine in St. Louis played a major role in the international effort, as they did in the sequencing and mapping of the human genome.

St. Louis, August 4, 2002 -- A physical map of the genetic makeup of a mouse--the mouse genome--is 98 percent complete and is being released online by the journal Nature. Researchers at the Genome Sequencing Center at Washington University School of Medicine in St. Louis played a major role in the international effort, as they did in the sequencing and mapping of the human genome.

Related Articles


"The mouse plays a vital role in research on human biology and disease," says John D. McPherson, Ph.D., associate professor of genetics and the lead investigator on the St. Louis team. "This physical map gives us the big picture of the mouse genome. It will be tremendously helpful to medical investigators and to those studying the human genome."

Comparison of the mouse and human maps, for example, can highlight regions of DNA that control genes. These regions are crucial to understanding the role of genes in health and disease, but they are difficult to find using current methods.

The physical mouse-genome map is a complementary effort to the draft sequence of the mouse genome, which was released last May. The important difference is one of detail and organization, says McPherson.

The draft sequence is a description of the chemical bases--represented by A, C, G, and T--that make up the genome. The physical map organizes and delineates this information on the mouse's 20 chromosomes. McPherson compared the draft sequence to loose pages from an encyclopedia. The pages may provide a lot of information, but they lack context.

"Each page may provide many details," he says, "like the population and climate of a country. But until all the pages are assembled correctly, you may not know that you are reading about Zaire." A physical map places all the "pages" of DNA sequence in their correct order within each volume, with each volume being a chromosome.

Furthermore, the DNA-sequence information used to compile the physical map was gathered differently from the information used to compile the draft sequence. Because the physical map comes from a separate source of genetic information, the researchers are using it to confirm the accuracy of the draft sequence.

"We are comparing the two independent data sets to be certain they are giving us the same answer," says McPherson.

The physical map benefits medical researchers in another way, as well. It was assembled using longer segments of DNA than those used to assemble the draft sequence. The long segments were grown, or cloned, in bacteria. Now that the mapping is complete, the bacteria containing these bits of mouse genome continue to be grown, stored in freezers, and carefully cataloged. Investigators studying mouse genes or regions of DNA now can locate the location of that particular segment on the map and obtain the actual clone of that region to study, rather than isolating the region themselves.

The following centers contributed to the project:

The Wellcome Trust Sanger Institute, Hinxton, Cambridge, England (http://www.sanger.ac.uk)

Genome Sequencing Center, Washington University School of Medicine, St Louis (http://genome.wustl.edu/)

Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada (http://www.bcgsc.bc.ca/)

The Institute for Genome Research, Rockville, MD (http://www.tigr.org/)

Children's Hospital Oakland Research Institute, Oakland, CA (http://www.childrenshospitaloakland.org/)

EMBL--European Bioinformatics Institute, Hinxton, Cambridge, UK (http://www.ebi.ac.uk)

Department of Electrical Engineering, Washington University, St Louis (http://www.ee.washington.edu/)

The Genome Sequencing Center (GSC) at Washington University School of Medicine in St. Louis focuses on the large scale generation and analysis of DNA sequence. Founded in 1993, the GSC is one of the top sequencing centers in the United States.

A physical map of the mouse genome. http://www.nature.com or http://dx.doi.org/10.1038/nature00957 (Nature subscribers can view the full text; all others can view the abstract).

Funding from the Wellcome Trust and the National Institutes of Health supported this research.


Story Source:

The above story is based on materials provided by Washington University School Of Medicine. Note: Materials may be edited for content and length.


Cite This Page:

Washington University School Of Medicine. "Physical Map Of Mouse Genome Now Available." ScienceDaily. ScienceDaily, 7 August 2002. <www.sciencedaily.com/releases/2002/08/020807065132.htm>.
Washington University School Of Medicine. (2002, August 7). Physical Map Of Mouse Genome Now Available. ScienceDaily. Retrieved November 29, 2014 from www.sciencedaily.com/releases/2002/08/020807065132.htm
Washington University School Of Medicine. "Physical Map Of Mouse Genome Now Available." ScienceDaily. www.sciencedaily.com/releases/2002/08/020807065132.htm (accessed November 29, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Saturday, November 29, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Rural India's Low-Cost Sanitary Pad Revolution

Rural India's Low-Cost Sanitary Pad Revolution

AFP (Nov. 28, 2014) — One man hopes his invention -– a machine that produces cheap sanitary pads –- will help empower Indian women. Duration: 01:51 Video provided by AFP
Powered by NewsLook.com
Research on Bats Could Help Develop Drugs Against Ebola

Research on Bats Could Help Develop Drugs Against Ebola

AFP (Nov. 28, 2014) — In Africa's only biosafety level 4 laboratory, scientists have been carrying out experiments on bats to understand how virus like Ebola are being transmitted, and how some of them resist to it. Duration: 01:18 Video provided by AFP
Powered by NewsLook.com
WHO Says Male Ebola Survivors Should Abstain From Sex

WHO Says Male Ebola Survivors Should Abstain From Sex

Newsy (Nov. 28, 2014) — WHO cites four studies that say Ebola can still be detected in semen up to 82 days after the onset of symptoms. Video provided by Newsy
Powered by NewsLook.com
Ebola Leaves Orphans Alone in Sierra Leone

Ebola Leaves Orphans Alone in Sierra Leone

AFP (Nov. 27, 2014) — The Ebola epidemic sweeping Sierra Leone is having a profound effect on the country's children, many of whom have been left without any family members to support them. Duration: 01:02 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins