Featured Research

from universities, journals, and other organizations

Growing Human Antibodies In Algae: Inexpensive And Fast Route To Large-scale Production, Say Scripps Scientists

Date:
January 16, 2003
Source:
Scripps Research Institute
Summary:
A group of scientists at The Scripps Research Institute (TSRI) have used algae to express an antibody that targets herpes virus, describing the work in an upcoming issue of the journal Proceedings of the National Academy of Sciences.

A group of scientists at The Scripps Research Institute (TSRI) have used algae to express an antibody that targets herpes virus, describing the work in an upcoming issue of the journal Proceedings of the National Academy of Sciences.

Related Articles


This antibody could potentially be an ingredient in an anti-herpes topical cream or other anti-herpes treatments, but more importantly the algae expression technology that the TSRI team used could facilitate production of any number of human antibodies and other proteins on a massive scale.

"This is a fast, new, effective way to make human therapeutic proteins," says TSRI Associate Professor Stephen P. Mayfield, Ph.D., who conducted the research with Research Associate Scott E. Franklin, Ph.D., and TSRI President Richard A. Lerner, M.D.

Significantly, the researchers were able to produce the antibody at a much lower cost than has been achieved in the past. In fact, they say they can now make antibodies, soluble receptors, and other proteins so much more cheaply that an entire new class of therapeutics may become accessible.

"You can't make [a drug] if the time and expense is such that you have to sell that drug for hundreds of thousands of dollars," says Mayfield. "This has to be the way we make drugs in the future."

From Pond Scum to Pharmacy Shelf

Also called immunoglobulins, antibodies are proteins produced by immune cells that are designed to recognize a wide range of foreign pathogens. After a bacterium, virus, or other pathogen enters the bloodstream, antibodies target antigens--proteins, carbohydrate molecules, and other pieces of the pathogen--specific to that foreign invader. These antibodies then alert the immune system to the presence of the invaders and attract lethal "effector" immune cells to the site of infection.

Antibodies can also be useful as therapeutics for a number of human diseases ranging from rheumatoid arthritis to leukemia. Likewise, there are many other human proteins that could potentially be used as drugs.

In fact, there may be over 200 proteins that could potentially be new anti-cancer, anti-inflammatory, anti-arthritis compounds, says Mayfield. As an example, an anti-IgE antibody, termed Omalizumab, has already shown great efficacy in human clinical trials for the treatment of allergic rhinitis and asthma. Unfortunately, the costs of producing the antibody, coupled with the relatively small amounts which can be produced with current technologies, has severely limited its availability.

In cases where scientists want to make an abundance of proteins, they often turn to the simplest expression system--bacteria. However, this does not work for large, complicated proteins like antibodies because bacteria do not have the machinery to assemble them into the correct structure. So large proteins are usually produced through an expensive and complicated process involving the fermentation of mammalian cells.

Algae may offer a cheaper and easier way to produce the proteins. Since algae grow naturally and use carbon dioxide from the air as a carbon source and sunlight as an energy source, whole ponds--tens of thousands of liters--of the algae can be grown once they are modified to produce the protein of interest.

"The scale on which you can grow these algae is enormous," notes Franklin.

Modifying the algae to produce proteins entails inserting a gene into the genome of the chloroplast, the organelles within the alga cell that converts sunlight and carbon dioxide into plant matter. The algae then express and assemble the antibodies within the chloroplasts, which can later be purified, intact.

Now that the researchers have established the fundamental technology, they are looking at applying it to any number of proteins and receptors.

"We think we can now put in pretty much any gene that we want and have it express," says Mayfield.

The article, "Expression and assembly of a fully active antibody in algae," authored by Stephen P. Mayfield, Scott E. Franklin, and Richard A. Lerner, is available online at: http://www.pnas.org/cgi/content/abstract/0237108100v1, and will be published in the journal Proceedings of the National Academy of Sciences on January 21, 2003.

This work was supported by funds from Sea Grant, the National Institutes of Health, and Syngenta Corporation.


Story Source:

The above story is based on materials provided by Scripps Research Institute. Note: Materials may be edited for content and length.


Cite This Page:

Scripps Research Institute. "Growing Human Antibodies In Algae: Inexpensive And Fast Route To Large-scale Production, Say Scripps Scientists." ScienceDaily. ScienceDaily, 16 January 2003. <www.sciencedaily.com/releases/2003/01/030116074422.htm>.
Scripps Research Institute. (2003, January 16). Growing Human Antibodies In Algae: Inexpensive And Fast Route To Large-scale Production, Say Scripps Scientists. ScienceDaily. Retrieved March 29, 2015 from www.sciencedaily.com/releases/2003/01/030116074422.htm
Scripps Research Institute. "Growing Human Antibodies In Algae: Inexpensive And Fast Route To Large-scale Production, Say Scripps Scientists." ScienceDaily. www.sciencedaily.com/releases/2003/01/030116074422.htm (accessed March 29, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Sunday, March 29, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

S. Leone in New Anti-Ebola Lockdown

S. Leone in New Anti-Ebola Lockdown

AFP (Mar. 28, 2015) — Sierra Leone imposed a three-day nationwide lockdown Friday for the second time in six months in a bid to prevent a resurgence of the deadly Ebola virus. Duration: 01:17 Video provided by AFP
Powered by NewsLook.com
These Popular Antibiotics Can Cause Permanent Nerve Damage

These Popular Antibiotics Can Cause Permanent Nerve Damage

Newsy (Mar. 27, 2015) — A popular class of antibiotic can leave patients in severe pain and even result in permanent nerve damage. Video provided by Newsy
Powered by NewsLook.com
WH Plan to Fight Antibiotic-Resistant Germs

WH Plan to Fight Antibiotic-Resistant Germs

AP (Mar. 27, 2015) — The White House on Friday announced a five-year plan to fight the threat posed by antibiotic-resistant bacteria amid fears that once-treatable germs could become deadly. (March 27) Video provided by AP
Powered by NewsLook.com
House Ready to Pass Medicare Doc Bill

House Ready to Pass Medicare Doc Bill

AP (Mar. 26, 2015) — In rare bipartisan harmony, congressional leaders pushed a $214 billion bill permanently blocking physician Medicare cuts toward House passage Thursday, moving lawmakers closer to resolving a problem that has plagued them for years. (March 26) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins