Featured Research

from universities, journals, and other organizations

Hot Proteins May Sharpen DNA Tests; Help Bioprocessing

Date:
June 24, 2003
Source:
University Of Maryland Biotechnology Institute
Summary:
Unique heat shock proteins that protect bacteria in undersea hot vents could be used to improve the sensitivity of PCR, the common technique used to amplify bits of DNA for medical and forensic sciences, say researchers with the University of Maryland Biotechnology Institute (UMBI).

WASHINGTON, D.C., BIO2003- Unique heat shock proteins that protect bacteria in undersea hot vents could be used to improve the sensitivity of PCR, the common technique used to amplify bits of DNA for medical and forensic sciences, say researchers with the University of Maryland Biotechnology Institute (UMBI).

In addition, commercial manufacturing of vaccines, antibiotics and other medically important proteins could become more efficient by incorporating the unique heat shock proteins into microbial cells in those processes. Future use of the proteins could also impact agriculture, fish production and other commercially important biological processes.

Microorganisms that live in deep ocean vents where water exceeds the normal boiling point possess a phenomenal capacity for resisting heat damage." says Frank Robb of UMBI's Center of Marine Biotechnology. When temperatures rise above 103 ' C, they form heat shock proteins that work as chaperones of key proteins in the cell, protecting and allowing them to remain intact at very high temperatures.

Similar chaperone proteins appear in all branches of life. In humans they help prevent cataracts by keeping the proteins in the eye lens fluids from deteriorating. The polymerase chain reaction, or PCR, which is commonly used in clinical medicine, forensic science, evolutionary biology and archeology, and genetic disease diagnostics, relies on a heat stable DNA polymerase.

Robb explains recent experiments at COMB, "We have been able to show that by adding a small heat shock protein from Pyrococcus furiosus to the DNA polymerase enzymes used in the PCR test, you can increase the sensitivity by about ten times.

In addition, when we put the gene encoding these unique chaperone proteins into E. coli, a common bacterium, the resulting genetically modified E. coli strain could grow and survive at higher temperatures," says Robb.

This work is published in: Laksanalamai P, Jiemjit A, Bu Z, Maeder L, Robb FT. (2003) Multi-subunit assembly of the Pyrococcus furiosus small heat shock protein is essential for cellular protection at high temperature. Extremophiles, 7:79-83.

UMBI has patented the small heat shock proteins from P. furiosus. For information on licensing technology, please contact Claude Nash, Vice President Business Development at nash@umbi.umd.edu or 410-385-6328.

The University of Maryland Biotechnology Institute was mandated by the state of Maryland legislature in 1985 as "a new paradigm of state economic development in biotech-related sciences." With five major research and education centers across Maryland, UMBI is dedicated to advancing the frontiers of biotechnology. The centers are the Center for Advanced Research in Biotechnology in Rockville; Center for Biosystems Research in College Park; and Center of Marine Biotechnology, Medical Biotechnology Center, and the Institute of Human Virology, all in Baltimore.


Story Source:

The above story is based on materials provided by University Of Maryland Biotechnology Institute. Note: Materials may be edited for content and length.


Cite This Page:

University Of Maryland Biotechnology Institute. "Hot Proteins May Sharpen DNA Tests; Help Bioprocessing." ScienceDaily. ScienceDaily, 24 June 2003. <www.sciencedaily.com/releases/2003/06/030624090149.htm>.
University Of Maryland Biotechnology Institute. (2003, June 24). Hot Proteins May Sharpen DNA Tests; Help Bioprocessing. ScienceDaily. Retrieved July 26, 2014 from www.sciencedaily.com/releases/2003/06/030624090149.htm
University Of Maryland Biotechnology Institute. "Hot Proteins May Sharpen DNA Tests; Help Bioprocessing." ScienceDaily. www.sciencedaily.com/releases/2003/06/030624090149.htm (accessed July 26, 2014).

Share This




More Matter & Energy News

Saturday, July 26, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Europe's Highest Train Turns 80 in French Pyrenees

Europe's Highest Train Turns 80 in French Pyrenees

AFP (July 25, 2014) Europe's highest train, the little train of Artouste in the French Pyrenees, celebrates its 80th birthday. Duration: 01:05 Video provided by AFP
Powered by NewsLook.com
TSA Administrator on Politics and Flight Bans

TSA Administrator on Politics and Flight Bans

AP (July 24, 2014) TSA administrator, John Pistole's took part in the Aspen Security Forum 2014, where he answered questions on lifting of the ban on flights into Israel's Tel Aviv airport and whether politics played a role in lifting the ban. (July 24) Video provided by AP
Powered by NewsLook.com
Creative Makeovers for Ugly Cellphone Towers

Creative Makeovers for Ugly Cellphone Towers

AP (July 24, 2014) Mobile phone companies and communities across the country are going to new lengths to disguise those unsightly cellphone towers. From a church bell tower to a flagpole, even a pencil, some towers are trying to make a point. (July 24) Video provided by AP
Powered by NewsLook.com
Algonquin Power Goes Activist on Its Target Gas Natural

Algonquin Power Goes Activist on Its Target Gas Natural

TheStreet (July 23, 2014) When The Deal's Amanda Levin exclusively reported that Gas Natural had been talking to potential suitors, the Ohio company responded with a flat denial, claiming its board had not talked to anyone about a possible sale. Lo and behold, Canadian utility Algonquin Power and Utilities not only had approached the company, but it did it three times. Its last offer was for $13 per share as Gas Natural's was trading at a 60-day moving average of about $12.50 per share. Now Algonquin, which has a 4.9% stake in Gas Natural, has taken its case to shareholders, calling on them to back its proposals or, possibly, a change in the target's board. Video provided by TheStreet
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins