Featured Research

from universities, journals, and other organizations

The Trojan Clot-buster: Drug-coated Red Blood Cells Destroy Blood Clots From Within

Date:
August 12, 2003
Source:
University Of Pennsylvania Medical Center
Summary:
Thrombosis – the formation of internal blood clots – is a common cause of complications and even death following surgery. To create a better means of preventing thrombosis, researchers at University of Pennsylvania School of Medicine coated red blood cells (RBCs) with tissue plasminogen activator (tPA), a clot-dissolving drug commonly used as an emergency treatment for stroke.

Philadelphia, PA – Thrombosis – the formation of internal blood clots – is a common cause of complications and even death following surgery. To create a better means of preventing thrombosis, researchers at University of Pennsylvania School of Medicine coated red blood cells (RBCs) with tissue plasminogen activator (tPA), a clot-dissolving drug commonly used as an emergency treatment for stroke. When given alone, tPA has a short life span in circulation and has the potential to cause serious bleeding as it diffuses out of the bloodstream. The RBC/tPA combo, however, lasts ten times longer in the bloodstream than free-floating tPA and decreases the likelihood of excess bleeding, according to a new study.

"The idea of coating red blood cells with tPA was to create a Trojan Horse, a vehicle for sneaking tPA into the bloodstream that could not only add to the drug's longevity, but would also allow it to be incorporated into a growing blood clot. RBC/tPA can dissolve blood clots from within," said Vladimir R, Muzykantov, MD, PhD, associate professor in Penn's Department of Pharmacology and author of the study. "Our research shows that the Trojan Horse approach converts tPA into a potent killer of nascent blood clots, one that would pose a much smaller risk of causing internal bleeding."

In the August issue of Nature Biotechnology, Muzykantov and his colleagues demonstrate in animal models how the marriage of red blood cells and tPA has the potential of safely preventing thrombosis following surgery and as a therapeutic for victims of heart attack or stoke.

"If developed for humans, the RBC/tPA method could provide an ideal way of delivering clot-busting drugs, with fewer side effects," said Muzykantov. "In theory, patients could donate blood before surgery and receive their own cells bound to tPA following surgery, providing a safer alternative to blood-thinning medication."

Research has shown that preventing thrombosis helps to reduce mortality and morbidity in many diseases. Unfortunately, current clot-busting drugs have the tendency to cause excessive bleeding, either by causing bleeding outside of the blood vessels or by removing pre-existing and, perhaps, beneficial blood clots. According to the Penn researchers, RCB/tPA spares existing blood clots and is too large to cause damage outside of the bloodstream.

To coat red blood cells with tPA, Muzykantov and his colleagues capitalized on the 'stickiness' of streptavidin-biotin, a protein complex used in laboratories to study molecular interactions. Streptavidin forms an incredibly tight bond to a tiny molecule called biotin, so the researchers 'biotinylated' tPA and RBCs and used streptavidin to link them together. According to the researchers, the technique may provide a safe way of extending the longevity and safety of drugs within the circulatory system.

"Red blood cells can travel hundreds of kilometers throughout the blood vessels during their 100-or so day life-span. That fact alone makes the idea of RBC-bound therapeutics very interesting," said Muzykantov. "Moreover, red blood cells are relatively large, which makes it very difficult for drugs bound to them to burrow their way out of the bloodstream where they could potentially do damage."


Story Source:

The above story is based on materials provided by University Of Pennsylvania Medical Center. Note: Materials may be edited for content and length.


Cite This Page:

University Of Pennsylvania Medical Center. "The Trojan Clot-buster: Drug-coated Red Blood Cells Destroy Blood Clots From Within." ScienceDaily. ScienceDaily, 12 August 2003. <www.sciencedaily.com/releases/2003/08/030812073529.htm>.
University Of Pennsylvania Medical Center. (2003, August 12). The Trojan Clot-buster: Drug-coated Red Blood Cells Destroy Blood Clots From Within. ScienceDaily. Retrieved July 25, 2014 from www.sciencedaily.com/releases/2003/08/030812073529.htm
University Of Pennsylvania Medical Center. "The Trojan Clot-buster: Drug-coated Red Blood Cells Destroy Blood Clots From Within." ScienceDaily. www.sciencedaily.com/releases/2003/08/030812073529.htm (accessed July 25, 2014).

Share This




More Health & Medicine News

Friday, July 25, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

New Painkiller Designed To Discourage Abuse: Will It Work?

New Painkiller Designed To Discourage Abuse: Will It Work?

Newsy (July 24, 2014) The FDA approved Targiniq ER on Wednesday, a painkiller designed to keep users from abusing it. Like any new medication, however, it has doubters. Video provided by Newsy
Powered by NewsLook.com
Doctor At Forefront Of Fighting Ebola Outbreak Gets Ebola

Doctor At Forefront Of Fighting Ebola Outbreak Gets Ebola

Newsy (July 24, 2014) Sheik Umar Khan has treated many of the people infected in the Ebola outbreak, and now he's become one of them. Video provided by Newsy
Powered by NewsLook.com
Condemned Man's US Execution Takes Nearly Two Hours

Condemned Man's US Execution Takes Nearly Two Hours

AFP (July 24, 2014) America's death penalty debate raged Thursday after it took nearly two hours for Arizona to execute a prisoner who lost a Supreme Court battle challenging the experimental lethal drug cocktail. Duration: 00:55 Video provided by AFP
Powered by NewsLook.com
Can Watching TV Make You Feel Like A Failure?

Can Watching TV Make You Feel Like A Failure?

Newsy (July 24, 2014) A study by German researchers claims watching TV while you're stressed out can make you feel guilty and like a failure. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

    Health News

      Environment News

        Technology News



          Save/Print:
          Share:

          Free Subscriptions


          Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

          Get Social & Mobile


          Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

          Have Feedback?


          Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
          Mobile: iPhone Android Web
          Follow: Facebook Twitter Google+
          Subscribe: RSS Feeds Email Newsletters
          Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins