Featured Research

from universities, journals, and other organizations

Scientists Devise Method To Study Membrane Proteins, One Key To Drug Discovery

Date:
April 9, 2004
Source:
University Of Virginia Health System
Summary:
Scientists at the University of Virginia Health System have come up with a protocol to extract proteins from membranes by using chemicals that allow them to be reversibly folded and refolded.

CHARLOTTESVILLE, Va., – Proteins are complex compounds that are essential for the growth and repair of tissue. While scientists understand much about the structure and stability of water-soluble proteins that 'float' around in humans, they know very little about the shape and stability of proteins that are embedded, or folded, into the lipid membrane of cells. Such membrane proteins make up about 30 percent of all proteins in the body.

Despite years of research, scientists were struggling to devise a method to keep membrane proteins stable enough for long-term structural and functional studies. Now, scientists at the University of Virginia Health System have come up with a protocol to extract proteins from membranes by using chemicals that allow them to be reversibly folded and refolded. The proteins can then be studied using crystallography or nuclear magnetic resonance imaging. Their work is detailed in the March 23 issue of the "Proceedings of the National Academy of Sciences" (PNAS) and also on the cover of the journal. The paper can be found on the web at: http://www.pnas.org/cgi/content/full/101/12/4065.

"The majority of drugs on the market today are effective because they work on membrane proteins, but our basic knowledge about these proteins lags far behind that of water-soluble proteins," said Lukas Tamm, professor of molecular physiology and biological physics at U.Va. "We need to develop systems to get enough of these membrane proteins expressed in a cell culture so we can measure their thermodynamic, or energetic, stability," Tamm said. "This is of practical interest in designing proteins for therapeutic applications because the proteins need to be kept around for a long time. This protocol developed at U.Va. shows for the first time that these proteins can be taken out of their membrane environment and put back in without losing function," Tamm said. "We also found that the thermodynamic stability, or energy difference, between the folded and unfolded form of membrane proteins depends on the strength of the membrane "rubber band" that the proteins sit in. This energy difference can be predicted, one key variable in the drug discovery process."

In a commentary on the findings, also in the March 23 issue of PNAS, James Bowie, a professor with the Molecular Biology Institute at the University of California, Los Angeles, wrote that "the new work opens another door to a more quantitative description of the energetics protein-protein and protein-lipid interactions in the (membrane) bilayer. We are finally beginning to obtain quantitative information about membrane protein structure."

Working with U.Va. colleague Heedeok Hong, Tamm used an aqueous (water) system and a compound called urea, that unravels proteins, to carry out folding studies on a membrane protein of the Escherichia coli bacterium called OmpA. Tamm and Hong demonstrated that the folding of OmpA into the lipid bilayers of a membrane is a reversible, two-state process. They also demonstrated that elastic forces in bilayers, such as curvature stress, can affect the folding of membrane proteins.


Story Source:

The above story is based on materials provided by University Of Virginia Health System. Note: Materials may be edited for content and length.


Cite This Page:

University Of Virginia Health System. "Scientists Devise Method To Study Membrane Proteins, One Key To Drug Discovery." ScienceDaily. ScienceDaily, 9 April 2004. <www.sciencedaily.com/releases/2004/04/040409092532.htm>.
University Of Virginia Health System. (2004, April 9). Scientists Devise Method To Study Membrane Proteins, One Key To Drug Discovery. ScienceDaily. Retrieved July 23, 2014 from www.sciencedaily.com/releases/2004/04/040409092532.htm
University Of Virginia Health System. "Scientists Devise Method To Study Membrane Proteins, One Key To Drug Discovery." ScienceDaily. www.sciencedaily.com/releases/2004/04/040409092532.htm (accessed July 23, 2014).

Share This




More Matter & Energy News

Wednesday, July 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Robot Parking Valet Creates Stress-Free Travel

Robot Parking Valet Creates Stress-Free Travel

AP (July 23, 2014) 'Ray' the robotic parking valet at Dusseldorf Airport in Germany lets travelers to avoid the hassle of finding a parking spot before heading to the check-in desk. (July 23) Video provided by AP
Powered by NewsLook.com
Boeing Ups Outlook on 52% Profit Jump

Boeing Ups Outlook on 52% Profit Jump

Reuters - Business Video Online (July 23, 2014) Commercial aircraft deliveries rose seven percent at Boeing, prompting the aerospace company to boost full-year profit guidance- though quarterly revenues missed analyst estimates. Bobbi Rebell reports. Video provided by Reuters
Powered by NewsLook.com
Europe's Car Market on the Rebound?

Europe's Car Market on the Rebound?

Reuters - Business Video Online (July 23, 2014) Daimler kicks off a round of second-quarter earnings results from Europe's top carmakers with a healthy set of numbers - prompting hopes that stronger sales in Europe will counter weakness in emerging markets. Hayley Platt reports. Video provided by Reuters
Powered by NewsLook.com
9/11 Commission Members Warn of Terror "fatigue" Among American Public

9/11 Commission Members Warn of Terror "fatigue" Among American Public

Reuters - US Online Video (July 22, 2014) Ten years after releasing its initial report, members of the 9/11 Commission warn of the "waning sense of urgency" in combating terrorists attacks. Mana Rabiee reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins