Rosanna Zanetti-Dällenbach from the Women's University Hospital in Basel, Switzerland and colleagues from Stiftung Tumorbank, OncoScore AG and University Hospital in Basel, analysed the gene expression profile of core needle biopsies taken from 22 women diagnosed with breast cancer. For each woman, they compared the biopsy expression profile with the expression profile of a surgical sample taken from the tumour subsequently to the core needle biopsy. Zanetti-Dällenbach et al. quantified the expression of 60 genes known to be involved in breast tumour development using a technique called reverse polymerase chain reaction (PCR). Zanetti-Dällenbach et al. also analysed the gene expression profiles of surgical samples taken from the breast tumours of 317 patients who did not undergo a core needle biopsy.

The results of Zanetti-Dällenbach et al.'s study show that the gene expression levels of the core biopsy and the surgical sample are identical for most women. But the authors find that the expression of four specific genes is significantly increased in the surgical samples compared to the core needle biopsies. In the group of women who did not get a core needle biopsy, however, the expression of these four genes is not increased. These four genes are involved in inflammation and wound repair as well as tumour invasion and metastasis. The authors conclude that their expression must therefore be modified by the core needle biopsy procedure itself. They warn that care should be taken when interpreting the gene expression profile of a surgical sample carried out following a core biopsy. Although the tumor's aggressiveness has not changed, the modified profile in surgical samples obtained after core needle biopsy might influence data interpretation with respect to prediction of risk assessment and treatment response. The core needle biopsy sample may give a cleaner, more accurate and more representative profile of the levels of gene expression in the tumour.

Article: Comparison of gene expression profiles in core biopsies and corresponding surgical breast cancer samples Authors: Rosanna Zanetti-Dallenbach, Vincent Vuaroqueaux, Edward Wight, Martin Labuhn, Gad Singer, Patrick Urban, Urs Eppenberger, Wolfgang Holzgreve and Serenella Eppenberger-Castori

Breast Cancer Research, 2006 (in press) http://breast-cancer-research.com/

Note: If no author is given, the source is cited instead.

• Breast Cancer
• Personalized Medicine
• Human Biology
• Women's Health
• Cancer
• Prostate Cancer

• Breast cancer
• DNA microarray
• Mammography
• Interventional radiology
• Metastasis
• Tumor suppressor gene