Featured Research

from universities, journals, and other organizations

Structure Of Largest Nonvirus Particle Ever Crystallized Modeled

Date:
November 27, 2007
Source:
University of California - Los Angeles
Summary:
Researchers have modeled the structure of the largest cellular structure ever crystallized, suggesting ways to engineer the particles for drug delivery. The research study focuses on new engineered nanomaterial vaults for use as a drug-therapy vehicle.

Model of the vault derived by UCLA researchers, with applications to drug delivery.
Credit: UCLA

Researchers at UCLA's California NanoSystems Institute, the David Geffen School of Medicine at UCLA and the Howard Hughes Medical Institute have modeled the structure of the largest cellular particle ever crystallized, suggesting ways to engineer the particles for drug delivery.

The research study focuses on new engineered nanomaterial vaults for use as a drug-therapy vehicle.

The team of researchers at UCLA is led by David Eisenberg and Leonard H. Rome of the departments of biological chemistry at the Geffen School of Medicine and the California NanoSystems Institute and associate researchers Daniel H. Anderson, Valerie A. Kickhoefer and Stuart A. Sievers. Eisenberg, Anderson and Sievers are also members of the Howard Hughes Medical Institute and the UCLA--U.S. Department of Energy Institute for Genomics and Proteomics.

Vaults are large, barrel-shaped particles found in the cytoplasm of all mammalian cells; they may function in innate immunity. As naturally occurring nanoscale capsules, vaults may be useful to engineer as therapeutic delivery vehicles. For the study, the team of researchers proposed an atomic structure for the thin outer shell of the vault.

Using X-ray diffraction and computer modeling, the research team developed a draft atomic model for the major vault protein, which forms the shell-like enclosure of the vault.

"Our draft model is essentially an atomic-level vault with a completely unique structure, like a barrel with staves. It is unlike any other large structure found in nature," Rome said. "The outside of the vault structure is like an eggshell -- a continuous protective barrier with no gaps."

The shell is made up of 96 identical protein chains -- each made of 873 amino acid residues -- folded into 14 domains. Each chain forms an elongated stave of half the vault, as well as the cap of the barrel-like shell.

"These nanostructured vaults offer a human-friendly nanocontainer, like a molecular-level C-5A transport jet, with a cargo hold large enough to encompass a whole ribosome with its hundreds of proteins and nucleic acids, or enough drugs to control a cell," Eisenberg said.

The construction of the draft atomic model lays the foundation for further studies of vaults and will guide vault engineering projects focused on the targeted release of vault contents for drug delivery.

Journal citation: Anderson DH, Kickhoefer VA, Sievers SA, Rome LH, Eisenberg D (2007) Draft crystal structure of the vault shell at 9-A resolution. PLoS Biol 5(11): e318. doi:10.1371/journal.pbio.0050318

The research is supported by a National Science Foundation Nanoscience Interdisciplinary Research Team Grant, the Howard Hughes Medical Institute, the National Institutes of Health and the Department of Energy.


Story Source:

The above story is based on materials provided by University of California - Los Angeles. Note: Materials may be edited for content and length.


Cite This Page:

University of California - Los Angeles. "Structure Of Largest Nonvirus Particle Ever Crystallized Modeled." ScienceDaily. ScienceDaily, 27 November 2007. <www.sciencedaily.com/releases/2007/11/071126201401.htm>.
University of California - Los Angeles. (2007, November 27). Structure Of Largest Nonvirus Particle Ever Crystallized Modeled. ScienceDaily. Retrieved September 1, 2014 from www.sciencedaily.com/releases/2007/11/071126201401.htm
University of California - Los Angeles. "Structure Of Largest Nonvirus Particle Ever Crystallized Modeled." ScienceDaily. www.sciencedaily.com/releases/2007/11/071126201401.htm (accessed September 1, 2014).

Share This




More Matter & Energy News

Monday, September 1, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Australian Airlines Relax Phone Ban Too

Australian Airlines Relax Phone Ban Too

Reuters - Business Video Online (Aug. 26, 2014) Qantas and Virgin say passengers can use their smartphones and tablets throughout flights after a regulator relaxed a ban on electronic devices during take-off and landing. As Hayley Platt reports the move comes as the two domestic rivals are expected to post annual net losses later this week. Video provided by Reuters
Powered by NewsLook.com
Hurricane Marie Brings Big Waves to California Coast

Hurricane Marie Brings Big Waves to California Coast

Reuters - US Online Video (Aug. 26, 2014) Huge waves generated by Hurricane Marie hit the Southern California coast. Rough Cut (no reporter narration). Video provided by Reuters
Powered by NewsLook.com
Chinese Researchers Might Be Creating Supersonic Submarine

Chinese Researchers Might Be Creating Supersonic Submarine

Newsy (Aug. 26, 2014) Chinese researchers have expanded on Cold War-era tech and are closer to building a submarine that could reach the speed of sound. Video provided by Newsy
Powered by NewsLook.com
Breakingviews: India Coal Strained by Supreme Court Ruling

Breakingviews: India Coal Strained by Supreme Court Ruling

Reuters - Business Video Online (Aug. 26, 2014) An acute coal shortage is likely to be aggravated as India's supreme court declared government coal allocations illegal, says Breakingviews' Peter Thal Larsen. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins