Featured Research

from universities, journals, and other organizations

New Cheaper Method For Mapping Disease Genes

Date:
May 27, 2008
Source:
Karolinska Institutet
Summary:
Scientists have developed a new DNA-sequencing method that is much cheaper than those currently in use in laboratories. They hope that this new method will make it possible to map disease genes in large patient groups, which in turn can mean quicker breakthroughs for new treatments for a wide variety of diseases.

Scientists at the Swedish medical university Karolinska Institutet have developed a new DNA-sequencing method that is much cheaper than those currently in use in laboratories. They hope that this new method will make it possible to map disease genes in large patient groups, which in turn can mean quicker breakthroughs for new treatments for a wide variety of diseases.

By mapping DNA, scientists can trace disease genes, understand how bacteria and viruses cause infection and chart the evolution of mankind and other species. When the HUGO project mapped the first human genome not so long ago, it cost over a billion kronor and took over ten years. Today, there are instruments on the market that can do the same thing in a matter of months for less then ten million kronor. However, if scientists are to have opportunities to study disease genes in detail, and from hundreds of patients, the process must be much, much cheaper.

A Swedish team, led by Sten Linnarsson at the Department of Medical Biochemistry and Biophysics at Karolinska Institutet, has now developed a new DNA-sequencing method that can one day make it possible to map out the human genome for one-tenth of today’s cost. The method is presented in the online edition of the scientific journal Nature Biotechnology.

The scientists took DNA from the enteric bacteria E. coli and split it into tiny fragments, each with a length of approximately 200 nucleotides (the building blocks of DNA: A, C, G and T). These fragments were then spread out and fixed onto a microscope slide so that several million fragments could be analysed simultaneously. These fragments were then rinsed in a fluid containing short DNA sequences of five nucleotides, marked with a fluorescent dye, which allowed them to examine which of the short DNA sequences adhered to each fragment.

After having rinsed all possible short DNA sequences over several million fragments, the scientists were able to then digitally piece together the sequences into one complete chain of the entire bacteria genome, a total of 4.5 million nucleotides long.

“Everything takes place in our own specially built instrument, which comprises a microscope powerful enough to take pictures of DNA fragments, an automated pipette and a small flow chamber with a glass surface on which the reaction itself occurs,” says Sten Linnarsson.

This is not the first time that Swedish scientists have successfully developed new methods of DNA sequencing. Ten years ago Pĺl Nyrén and his colleagues from the Royal Institute of Technology published Pyrosequencing, one of the most common methods of mapping DNA in use today.


Story Source:

The above story is based on materials provided by Karolinska Institutet. Note: Materials may be edited for content and length.


Journal Reference:

  1. Rapid genome sequencing with short universal tiling probes. Arno Pihlak, Göran Baurén, Ellef Hersoug, Peter Lönnerberg, Ats Metsis and Sten Linnarsson, Nature Biotechnology AOP, 25 May 2008. doi:10.1038/nbt1405.

Cite This Page:

Karolinska Institutet. "New Cheaper Method For Mapping Disease Genes." ScienceDaily. ScienceDaily, 27 May 2008. <www.sciencedaily.com/releases/2008/05/080526165347.htm>.
Karolinska Institutet. (2008, May 27). New Cheaper Method For Mapping Disease Genes. ScienceDaily. Retrieved July 31, 2014 from www.sciencedaily.com/releases/2008/05/080526165347.htm
Karolinska Institutet. "New Cheaper Method For Mapping Disease Genes." ScienceDaily. www.sciencedaily.com/releases/2008/05/080526165347.htm (accessed July 31, 2014).

Share This




More Health & Medicine News

Thursday, July 31, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Health Insurers' Profits Slide

Health Insurers' Profits Slide

Reuters - Business Video Online (July 30, 2014) — Obamacare-related costs were said to be behind the profit plunge at Wellpoint and Humana, but Wellpoint sees the new exchanges boosting its earnings for the full year. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com
Concern Grows Over Worsening Ebola Crisis

Concern Grows Over Worsening Ebola Crisis

AFP (July 30, 2014) — Pan-African airline ASKY has suspended all flights to and from the capitals of Liberia and Sierra Leone amid the worsening Ebola health crisis, which has so far caused 672 deaths in Guinea, Liberia and Sierra Leone. Duration: 00:43 Video provided by AFP
Powered by NewsLook.com
At Least 20 Chikungunya Cases in New Jersey

At Least 20 Chikungunya Cases in New Jersey

AP (July 30, 2014) — At least 20 New Jersey residents have tested positive for chikungunya, a mosquito-borne virus that has spread through the Caribbean. (July 30) Video provided by AP
Powered by NewsLook.com
Xtreme Eating: Your Daily Caloric Intake All On One Plate

Xtreme Eating: Your Daily Caloric Intake All On One Plate

Newsy (July 30, 2014) — The Center for Science in the Public Interest released its 2014 list of single meals with whopping calorie counts. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



      Save/Print:
      Share:  

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile iPhone Android Web
      Follow Facebook Twitter Google+
      Subscribe RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins