Featured Research

from universities, journals, and other organizations

Low Sensitivity Of Clinical Genomic Signatures

Date:
September 24, 2008
Source:
Annals of Clinical Microbiology and Antimicrobials
Summary:
Genomic signature sequences used by clinical researchers to detect, quantify and diagnose nucleic acid sequences are not inclusive enough. New research shows that many published sequences are of unacceptably low sensitivity for most clinical applications.

Genomic signature sequences used by clinical researchers to detect, quantify and diagnose nucleic acid sequences are not inclusive enough. New research shows that many published sequences are of unacceptably low sensitivity for most clinical applications.

Related Articles


According to Shea Gardner of the Lawrence Livermore National Laboratory, California, lead author of the study, "In recent years, real-time polymerase chain reaction (RT-PCR) has become a leading technique for nucleic acid detection and quantification. In our analysis of the RT-PCR signatures described in recent literature, we found that many published sequences have a high specificity but only a very low sensitivity".

Gardner and co-author, Gordon Lemmon, suggest that a rigorous approach involving false positive and false negative analysis should be the standard by which an initial assessment of signature quality is made. They write, "Signatures must be reassessed as new sequence data becomes available. For targets with wide nucleotide diversity, such as influenza viruses, it is necessary to develop a set of signatures with a minimal set clustering approach that may also include signatures with degenerate/inosine bases".

Gardner comments, "The rapidly growing availability of sequence data for pathogens from across the globe means that we can better predict how robustly a signature will detect all the intended targets. Using a computational approach to design and pre-screen signatures can improve the quality of sequence-based diagnostics and save time and money in lab testing".


Story Source:

The above story is based on materials provided by Annals of Clinical Microbiology and Antimicrobials. Note: Materials may be edited for content and length.


Journal Reference:

  1. Gordon H Lemmon and Shea N Gardner. Predicting the sensitivity and specificity of published real-time PCR assays. Annals of Clinical Microbiology and Antimicrobials, (in press)

Cite This Page:

Annals of Clinical Microbiology and Antimicrobials. "Low Sensitivity Of Clinical Genomic Signatures." ScienceDaily. ScienceDaily, 24 September 2008. <www.sciencedaily.com/releases/2008/09/080924192441.htm>.
Annals of Clinical Microbiology and Antimicrobials. (2008, September 24). Low Sensitivity Of Clinical Genomic Signatures. ScienceDaily. Retrieved November 27, 2014 from www.sciencedaily.com/releases/2008/09/080924192441.htm
Annals of Clinical Microbiology and Antimicrobials. "Low Sensitivity Of Clinical Genomic Signatures." ScienceDaily. www.sciencedaily.com/releases/2008/09/080924192441.htm (accessed November 27, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Thursday, November 27, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Pet Dogs to Be Used in Anti-Ageing Trial

Pet Dogs to Be Used in Anti-Ageing Trial

Reuters - Innovations Video Online (Nov. 26, 2014) Researchers in the United States are preparing to discover whether a drug commonly used in human organ transplants can extend the lifespan and health quality of pet dogs. Video provided by Reuters
Powered by NewsLook.com
Today's Prostheses Are More Capable Than Ever

Today's Prostheses Are More Capable Than Ever

Newsy (Nov. 26, 2014) Advances in prosthetics are making replacement body parts stronger and more lifelike than they’ve ever been. Video provided by Newsy
Powered by NewsLook.com
From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

Newsy (Nov. 25, 2014) The US FDA is announcing new calorie rules on Tuesday that will require everywhere from theaters to vending machines to include calorie counts. Video provided by Newsy
Powered by NewsLook.com
Daily Serving Of Yogurt Could Reduce Risk Of Type 2 Diabetes

Daily Serving Of Yogurt Could Reduce Risk Of Type 2 Diabetes

Newsy (Nov. 25, 2014) Need another reason to eat yogurt every day? Researchers now say it could reduce a person's risk of developing type 2 diabetes. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins