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New Tool Probes Function Of Rice Genes

Date:
October 12, 2008
Source:
Public Library of Science
Summary:
Researchers have developed a new tool for investigating the rice genome. The inexpensive, publicly-available rice DNA microarray covers nearly all the 45,000 genes in the rice genome.

A new tool for investigating the rice genome has been developed by researchers at UC Davis led by Pamela Ronald, professor of plant pathology. The inexpensive, publicly-available rice DNA microarray covers nearly all the 45,000 genes in the rice genome.

Details are published this week in the open-access journal PLoS ONE.

In higher organisms, such as humans or rice plants, each cell type express different genes at different times. Scientists have developed high-throughput methods to examine these gene expression profiles using "DNA Microarrays," thousands of fragments of DNA fixed to a glass slide. DNA microarrays can be used to figure out which genes are important for responding to a stimulus or tolerating stresses.

Ronald and her colleagues used the new rice microarray to investigate gene expression changes when plants are grown in the light versus the dark. They then combined this gene expression data with biochemical pathway data to correctly predict the function of genes whose role was previously unknown. The newly identified genes carry out light-related biochemical processes such as photosynthesis and photorespiration.

The methods and array developed in this paper will aid researchers in identifying the function of the 45,000 rice genes, only a few of which have so far been characterized, Ronald said. The group also has developed a web-based program that allows the user to compare gene expression profiles across multiple rice microarray platforms, which will further accelerate this research.


Story Source:

The above story is based on materials provided by Public Library of Science. Note: Materials may be edited for content and length.


Journal Reference:

  1. Jung K-H, Dardick C, Bartley LE, Cao P, Phetsom J, et al. Refinement of Light-Responsive Transcript Lists Using Rice Oligonucleotide Arrays: Evaluation of Gene-Redundancy. PLoS ONE, 2008; 3 (10): e3337 DOI: 10.1371/journal.pone.0003337

Cite This Page:

Public Library of Science. "New Tool Probes Function Of Rice Genes." ScienceDaily. ScienceDaily, 12 October 2008. <www.sciencedaily.com/releases/2008/10/081009094647.htm>.
Public Library of Science. (2008, October 12). New Tool Probes Function Of Rice Genes. ScienceDaily. Retrieved July 22, 2014 from www.sciencedaily.com/releases/2008/10/081009094647.htm
Public Library of Science. "New Tool Probes Function Of Rice Genes." ScienceDaily. www.sciencedaily.com/releases/2008/10/081009094647.htm (accessed July 22, 2014).

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