Featured Research

from universities, journals, and other organizations

Seeing The Unseen With 'Super-resolution' Fluorescence Microscopy

Date:
December 24, 2008
Source:
American Society for Cell Biology
Summary:
Thanks to new "super-resolution" fluorescence microscopy technique, researchers have succeeded in resolving features of cells as minuscule as 20-30 nanometers, an order of magnitude smaller than conventional fluorescence light microscopy images.

Thanks to a new "super-resolution" fluorescence microscopy technique, Harvard University researchers have succeeded in resolving the features of cells as miniscule as 20-30 nanometers (nm), an order of magnitude smaller than conventional fluorescence light microscopy images, according to a presentation at the American Society for Cell Biology (ASCB) 48th Annual Meeting, Dec. 13-17, 2008, in San Francisco.

Related Articles


"Super resolution" microscopy techniques enable scientists to visualize cells laterally below 200-300 nm, which is the length scale of most intracellular structures and the level at which the cell gets most of its work done.

Harvard's "super-resolution" technique, developed by Bo Huang, Xioawei Zhuang and colleagues at the university, is called Stochastic Optical Reconstruction Microscopy (STORM).

It is one of several higher-resolution fluorescence microscopy techniques that fundamentally surpass the diffraction "blind spot" of conventional light microscopes.

Because conventional light microscopes cannot resolve two objects closer than half the wavelength of the light, they produce images that appear blurry and overlap no matter how high the magnification.

According to the Harvard researchers, STORM can record light emitted from a single molecule in the sample.

Using probe molecules that can be "photoswitched" between a visible and an invisible state, STORM can determine the position of every molecule of interest and can then compile all the molecules' positions to define a structure.

Huang and colleagues have adapted STORM to study three-dimensional structures and can now visualize a whole cell with an axial resolution of 50-60 nm.

Multicolor imaging also has been achieved by using photoswitchable fluorophores made of combinatorial pairs of various activator dyes and reporter dyes. Multicolor, 3-D STORM is able to visualize detailed interactions between cell organelles and the cytoskeleton.

In brain tissue, the researchers used STORM to reveal the fine details in the synaptic structure of the olfactory system.

The lead author presented, "Seeing the Unseen in a Cell with Super-Resolution Fluorescence Microscopy," on Dec. 16 at the Moscone Center.

Authors: B. Huang, B. Brandenburg, X. Zhuang, Howard Hughes Medical Institute, Harvard University, Cambridge, MA; B. Huang, S.A. Jones, B. Brandenburg, X. Zhuang, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA; M. Bates, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA; W. Wang, X. Zhuang, Department of Physics, Harvard University, Cambridge, MA; G.T. Dempsey, Graduate Program in Biophysics, Harvard University, Cambridge, MA


Story Source:

The above story is based on materials provided by American Society for Cell Biology. Note: Materials may be edited for content and length.


Cite This Page:

American Society for Cell Biology. "Seeing The Unseen With 'Super-resolution' Fluorescence Microscopy." ScienceDaily. ScienceDaily, 24 December 2008. <www.sciencedaily.com/releases/2008/12/081216133434.htm>.
American Society for Cell Biology. (2008, December 24). Seeing The Unseen With 'Super-resolution' Fluorescence Microscopy. ScienceDaily. Retrieved February 27, 2015 from www.sciencedaily.com/releases/2008/12/081216133434.htm
American Society for Cell Biology. "Seeing The Unseen With 'Super-resolution' Fluorescence Microscopy." ScienceDaily. www.sciencedaily.com/releases/2008/12/081216133434.htm (accessed February 27, 2015).

Share This


More From ScienceDaily



More Matter & Energy News

Friday, February 27, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Vibrating Bicycle Senses Traffic

Vibrating Bicycle Senses Traffic

Reuters - Innovations Video Online (Feb. 26, 2015) Dutch scientists have developed a smart bicycle that uses sensors, wireless technology and video to warn riders of traffic dangers. Ben Gruber reports. Video provided by Reuters
Powered by NewsLook.com
In Japan, Robot Dogs Are for Life -- And Death

In Japan, Robot Dogs Are for Life -- And Death

AFP (Feb. 25, 2015) Robot dogs are the perfect pet for some in Japan who go to repairmen-turned-vets when their pooch breaks down - while a full Buddhist funeral ceremony awaits those who don&apos;t make it. Duration: 02:40 Video provided by AFP
Powered by NewsLook.com
London Show Dissects History of Forensic Science

London Show Dissects History of Forensic Science

AFP (Feb. 25, 2015) Forensic science, which has fascinated generations with its unravelling of gruesome crime mysteries, is being put under the microscope in an exhibition of real criminal investigations in London. Duration: 00:53 Video provided by AFP
Powered by NewsLook.com
Researchers Replace Damaged Hands With Prostheses

Researchers Replace Damaged Hands With Prostheses

Newsy (Feb. 25, 2015) Scientists in Austria have been able to fit patients who&apos;ve lost the use of a hand with bionic prostheses the patients control with their minds. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins