Featured Research

from universities, journals, and other organizations

Seeing The Unseen With 'Super-resolution' Fluorescence Microscopy

Date:
December 24, 2008
Source:
American Society for Cell Biology
Summary:
Thanks to new "super-resolution" fluorescence microscopy technique, researchers have succeeded in resolving features of cells as minuscule as 20-30 nanometers, an order of magnitude smaller than conventional fluorescence light microscopy images.

Thanks to a new "super-resolution" fluorescence microscopy technique, Harvard University researchers have succeeded in resolving the features of cells as miniscule as 20-30 nanometers (nm), an order of magnitude smaller than conventional fluorescence light microscopy images, according to a presentation at the American Society for Cell Biology (ASCB) 48th Annual Meeting, Dec. 13-17, 2008, in San Francisco.

"Super resolution" microscopy techniques enable scientists to visualize cells laterally below 200-300 nm, which is the length scale of most intracellular structures and the level at which the cell gets most of its work done.

Harvard's "super-resolution" technique, developed by Bo Huang, Xioawei Zhuang and colleagues at the university, is called Stochastic Optical Reconstruction Microscopy (STORM).

It is one of several higher-resolution fluorescence microscopy techniques that fundamentally surpass the diffraction "blind spot" of conventional light microscopes.

Because conventional light microscopes cannot resolve two objects closer than half the wavelength of the light, they produce images that appear blurry and overlap no matter how high the magnification.

According to the Harvard researchers, STORM can record light emitted from a single molecule in the sample.

Using probe molecules that can be "photoswitched" between a visible and an invisible state, STORM can determine the position of every molecule of interest and can then compile all the molecules' positions to define a structure.

Huang and colleagues have adapted STORM to study three-dimensional structures and can now visualize a whole cell with an axial resolution of 50-60 nm.

Multicolor imaging also has been achieved by using photoswitchable fluorophores made of combinatorial pairs of various activator dyes and reporter dyes. Multicolor, 3-D STORM is able to visualize detailed interactions between cell organelles and the cytoskeleton.

In brain tissue, the researchers used STORM to reveal the fine details in the synaptic structure of the olfactory system.

The lead author presented, "Seeing the Unseen in a Cell with Super-Resolution Fluorescence Microscopy," on Dec. 16 at the Moscone Center.

Authors: B. Huang, B. Brandenburg, X. Zhuang, Howard Hughes Medical Institute, Harvard University, Cambridge, MA; B. Huang, S.A. Jones, B. Brandenburg, X. Zhuang, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA; M. Bates, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA; W. Wang, X. Zhuang, Department of Physics, Harvard University, Cambridge, MA; G.T. Dempsey, Graduate Program in Biophysics, Harvard University, Cambridge, MA


Story Source:

The above story is based on materials provided by American Society for Cell Biology. Note: Materials may be edited for content and length.


Cite This Page:

American Society for Cell Biology. "Seeing The Unseen With 'Super-resolution' Fluorescence Microscopy." ScienceDaily. ScienceDaily, 24 December 2008. <www.sciencedaily.com/releases/2008/12/081216133434.htm>.
American Society for Cell Biology. (2008, December 24). Seeing The Unseen With 'Super-resolution' Fluorescence Microscopy. ScienceDaily. Retrieved September 2, 2014 from www.sciencedaily.com/releases/2008/12/081216133434.htm
American Society for Cell Biology. "Seeing The Unseen With 'Super-resolution' Fluorescence Microscopy." ScienceDaily. www.sciencedaily.com/releases/2008/12/081216133434.htm (accessed September 2, 2014).

Share This




More Matter & Energy News

Tuesday, September 2, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Australian Airlines Relax Phone Ban Too

Australian Airlines Relax Phone Ban Too

Reuters - Business Video Online (Aug. 26, 2014) Qantas and Virgin say passengers can use their smartphones and tablets throughout flights after a regulator relaxed a ban on electronic devices during take-off and landing. As Hayley Platt reports the move comes as the two domestic rivals are expected to post annual net losses later this week. Video provided by Reuters
Powered by NewsLook.com
Hurricane Marie Brings Big Waves to California Coast

Hurricane Marie Brings Big Waves to California Coast

Reuters - US Online Video (Aug. 26, 2014) Huge waves generated by Hurricane Marie hit the Southern California coast. Rough Cut (no reporter narration). Video provided by Reuters
Powered by NewsLook.com
Chinese Researchers Might Be Creating Supersonic Submarine

Chinese Researchers Might Be Creating Supersonic Submarine

Newsy (Aug. 26, 2014) Chinese researchers have expanded on Cold War-era tech and are closer to building a submarine that could reach the speed of sound. Video provided by Newsy
Powered by NewsLook.com
Breakingviews: India Coal Strained by Supreme Court Ruling

Breakingviews: India Coal Strained by Supreme Court Ruling

Reuters - Business Video Online (Aug. 26, 2014) An acute coal shortage is likely to be aggravated as India's supreme court declared government coal allocations illegal, says Breakingviews' Peter Thal Larsen. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins