Featured Research

from universities, journals, and other organizations

New TB Test Developed That Will Dramatically Cut Diagnosis Time

Date:
March 24, 2009
Source:
Albert Einstein College of Medicine
Summary:
Researchers have developed an on-site method to quickly diagnose tuberculosis (TB) and expose the deadly drug-resistant strains that can mingle undetected with treatable TB strains. Tiny viruses are engineered to inject TB bacteria with green-glowing protein for a fast, on-site diagnosis.

Researchers from the Albert Einstein College of Medicine and The University of Pittsburgh have developed an onsite method to quickly diagnose tuberculosis (TB) and expose the deadly drug-resistant strains that can mingle undetected with treatable TB strains.

The researchers engineered bacteriophages, tiny viruses that attack bacteria, with a green fluorescence protein (GFP) implanted in their genome. Bacteriophages spread by injecting their DNA into bacterial cells. In this case, the GFP gene accompanies the DNA of the phage into the Mycobacterium tuberculosis cell, the bacterium that causes TB, causing the cell to glow. A clinician could detect the glow with equipment available at many clinics.

"The development of these reporter flurophages allows us to bypass the existing method of diagnosing TB, which requires cultivating slow-growing bacteria in a biosafety level 3 environment, a time-consuming and costly process," says William R. Jacobs, Jr., Ph.D., one of the authors of the study. "By infecting live M. tuberculosis cells with a flurophage, a quick and highly sensitive visual reading can be done. We are optimistic that we can move the diagnostic process from several weeks to several days or even hours, which could have a significant impact on treatment."

"A report from South Africa showed that the extensively drug-resistant TB strains can kill within 16 days, on average," says Graham Hatfull, Ph.D., the lead author and close collaborator of Dr. Jacobs. "In rural Africa, it takes too long to collect samples, send them off, do the test, and have the data sent back. Clinicians need rapid, relatively cheap, and simple methods for detecting TB and drug-resistant strains in the local clinic. This test provides a quick diagnosis so the patient can be isolated and treated."

Besides quick diagnosis, the test also could be used to distinguish treatable TB strains from those that are drug resistant (DR-TB) and extensively drug resistant (XDR-TB), which normally takes months. Researchers treated M. tuberculosis with antibiotics at the same time the bacteriophages were introduced; the TB strains that were sensitive to antibiotics died, but the drug-resistant cells survived and continued to glow.

The group's research was funded as part of a major new research initiative from Howard Hughes Medical Institute (HHMI).


Story Source:

The above story is based on materials provided by Albert Einstein College of Medicine. Note: Materials may be edited for content and length.


Journal Reference:

  1. Fluoromycobacteriophages for Rapid, Specific, and Sensitive Antibiotic Susceptibility Testing of Mycobacterium tuberculosis. PLoS ONE, March 19, 2009

Cite This Page:

Albert Einstein College of Medicine. "New TB Test Developed That Will Dramatically Cut Diagnosis Time." ScienceDaily. ScienceDaily, 24 March 2009. <www.sciencedaily.com/releases/2009/03/090319132952.htm>.
Albert Einstein College of Medicine. (2009, March 24). New TB Test Developed That Will Dramatically Cut Diagnosis Time. ScienceDaily. Retrieved September 17, 2014 from www.sciencedaily.com/releases/2009/03/090319132952.htm
Albert Einstein College of Medicine. "New TB Test Developed That Will Dramatically Cut Diagnosis Time." ScienceDaily. www.sciencedaily.com/releases/2009/03/090319132952.htm (accessed September 17, 2014).

Share This



More Health & Medicine News

Wednesday, September 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

President To Send 3,000 Military Personnel To Fight Ebola

President To Send 3,000 Military Personnel To Fight Ebola

Newsy (Sep. 16, 2014) President Obama is expected to send 3,000 troops to West Africa as part of the effort to contain Ebola's spread. Video provided by Newsy
Powered by NewsLook.com
Man Floats for 31 Hours in Gulf Waters

Man Floats for 31 Hours in Gulf Waters

AP (Sep. 16, 2014) A Texas man is lucky to be alive after he and three others floated for more than a day in the Gulf of Mexico when their boat sank during a fishing trip. (Sept. 16) Video provided by AP
Powered by NewsLook.com
Ivorians Abandon Monkey Pets in Fear Over Ebola Virus

Ivorians Abandon Monkey Pets in Fear Over Ebola Virus

AFP (Sep. 16, 2014) Since the arrival of Ebola in Ivory Coast, Ivorians have been abandoning their pets, particularly monkeys, in the fear that they may transmit the virus. Duration: 00:47 Video provided by AFP
Powered by NewsLook.com
Study Links Male-Pattern Baldness To Prostate Cancer

Study Links Male-Pattern Baldness To Prostate Cancer

Newsy (Sep. 16, 2014) New findings suggest men with a certain type of baldness at age 45 are 39 percent more likely to develop aggressive prostate cancer. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

      Technology News



      Save/Print:
      Share:

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile: iPhone Android Web
      Follow: Facebook Twitter Google+
      Subscribe: RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins