Featured Research

from universities, journals, and other organizations

New Method For Detection Of Phosphoproteins Reveals Regulator Of Melanoma Invasion

Date:
April 17, 2009
Source:
Cell Press
Summary:
Scientists have developed a new approach for surveying phosphorylation, a process that is regulated by critical cell signaling pathways and regulates several key cellular signaling events. The research describes the regulation of a previously uncharacterized protein and demonstrates that it plays an important role in cancer cell invasion.

Scientists have developed a new approach for surveying phosphorylation, a process that is regulated by critical cell signaling pathways and regulates several key cellular signaling events. The research, published by Cell Press in the April 10th issue of the journal Molecular Cell, describes the regulation of a previously uncharacterized protein and demonstrates that it plays an important role in cancer cell invasion.

Related Articles


Many cancers, including melanoma, are associated with mutations in the gene encoding the protein kinase B-Raf. Kinases are proteins that regulate the function of other proteins by attaching a phosphate group to them. B-RAF mutations often lead to dysregulation of protein phosphorylation by the mitogen-activated protein (MAP) kinase signaling pathway. Identification and characterization of MAP kinase target proteins is critical for understanding the mechanisms involved in cancer progression.

"In contrast to targets regulated at the level of gene expression, little is known about how proteins are modified in response to oncogenic B-Raf signaling in melanoma cells. In particular, identifying cellular targets for phosphorylation is needed to gain a more comprehensive understanding of the responses to MAP kinase pathway dysregulation in melanoma," explains senior study author Dr. Natalie G. Ahn from the Department of Chemistry and Biochemistry at the University of Colorado and the Howard Hughes Medical Institute.

Current strategies to identify phosphorylated proteins require purification techniques to enrich phosphorylated from non-phosphorylated proteins and metabolic labeling procedures to quantify changes in phosphorylation. Unfortunately, these methods are not readily applied to all sample types. Dr. Ahn and colleagues developed a method for analyzing phosphorylated proteins in human cell extracts that does not depend on enrichment and can be performed quantitatively in a label-free manner.

Using their method, the researchers identified ninety phosphorylation events that were regulated by oncogenic B-Raf. The phosphorylated proteins included many known signaling molecules. However, one of the targets, MINERVA/FAM129B, belonged to a protein family with unknown function. Further investigation established a role for MAP kinase-dependent phosphorylation of MINERVA/FAM129B in cancer cell invasion within a three dimensional extracellular matrix environment.

"Our results revealed successful selection and sequencing of phosphopeptides in proteolytic digests without affinity enrichment, as well as label-free quantitation of regulated protein phosphorylation events," concludes Dr. Ahn. "Further, we demonstrated pathway-dependent phosphorylation of FAM129B and discovered its importance in controlling melanoma cell invasion."

The researchers include William M. Old, University of Colorado, Boulder, CO; John B. Shabb, University of North Dakota, Grand Forks, ND; Stephane Houel, University of Colorado, Boulder, CO, Howard Hughes Medical Institute; Hong Wang, University of Colorado, Boulder, CO; Kasey L. Couts, University of Colorado, Boulder, CO; Chia-yu Yen, University of Colorado, Boulder, CO; Elizabeth S. Litman, University of Colorado, Boulder, CO, Howard Hughes Medical Institute; Carrie H. Croy, University of Colorado, Boulder, CO, Howard Hughes Medical Institute; Karen Meyer-Arendt, University of Colorado, Boulder, CO; Jose G. Miranda, University of Colorado, Boulder, CO; Robert A. Brown, University of Colorado, Boulder, CO; Eric S. Witze, University of Colorado, Boulder, CO; Rebecca E. Schweppe, University of Colorado, Boulder, CO; Katheryn A. Resing, University of Colorado, Boulder, CO; and Natalie G. Ahn, University of Colorado, Boulder, CO, Howard Hughes Medical Institute.


Story Source:

The above story is based on materials provided by Cell Press. Note: Materials may be edited for content and length.


Journal Reference:

  1. Old et al. Functional Proteomics Identifies Targets of Phosphorylation by B-Raf Signaling in Melanoma. Molecular Cell, 2009; DOI: 10.1016/j.molcel.2009.03.007

Cite This Page:

Cell Press. "New Method For Detection Of Phosphoproteins Reveals Regulator Of Melanoma Invasion." ScienceDaily. ScienceDaily, 17 April 2009. <www.sciencedaily.com/releases/2009/04/090409134751.htm>.
Cell Press. (2009, April 17). New Method For Detection Of Phosphoproteins Reveals Regulator Of Melanoma Invasion. ScienceDaily. Retrieved December 20, 2014 from www.sciencedaily.com/releases/2009/04/090409134751.htm
Cell Press. "New Method For Detection Of Phosphoproteins Reveals Regulator Of Melanoma Invasion." ScienceDaily. www.sciencedaily.com/releases/2009/04/090409134751.htm (accessed December 20, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Saturday, December 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

The Best Tips to Curb Holiday Carbs

The Best Tips to Curb Holiday Carbs

Buzz60 (Dec. 19, 2014) It's hard to resist those delicious but fattening carbs we all crave during the winter months, but there are some ways to stay satisfied without consuming the extra calories. Vanessa Freeman (@VanessaFreeTV) has the details. Video provided by Buzz60
Powered by NewsLook.com
Sierra Leone Bikers Spread the Message to Fight Ebola

Sierra Leone Bikers Spread the Message to Fight Ebola

AFP (Dec. 19, 2014) More than 100 motorcyclists hit the road to spread awareness messages about Ebola. Nearly 7,000 people have now died from the virus, almost all of them in west Africa, according to the World Health Organization. Video provided by AFP
Powered by NewsLook.com
Researchers Test Colombian Village With High Alzheimer's Rates

Researchers Test Colombian Village With High Alzheimer's Rates

AFP (Dec. 19, 2014) In Yarumal, a village in N. Colombia, Alzheimer's has ravaged a disproportionately large number of families. A genetic "curse" that may pave the way for research on how to treat the disease that claims a new victim every four seconds. Duration: 02:42 Video provided by AFP
Powered by NewsLook.com
The Best Protein-Filled Foods to Energize You for the New Year

The Best Protein-Filled Foods to Energize You for the New Year

Buzz60 (Dec. 19, 2014) The new year is coming and nothing will energize you more for 2015 than protein-filled foods. Fitness and nutrition expert John Basedow (@JohnBasedow) gives his favorite high protein foods that will help you build muscle, lose fat and have endless energy. Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins