Featured Research

from universities, journals, and other organizations

Microscope Capable Of Live Imaging At Double The Resolution Of Fluorescence Microscopy Developed

Date:
May 11, 2009
Source:
University of Georgia
Summary:
Engineers have developed a microscope that is capable of live imaging at double the resolution of fluorescence microscopy using structured illumination.

Images of GFP-labeled microtubules. Top: the conventional image. Bottom: at 100nm resolution with structured illumination.
Credit: Image courtesy of University of Georgia

A crucial tool in the evolution of scientific capability in bioscience, the fluorescence microscope has allowed a generation of scientists to study the properties of proteins inside cells. Yet as human capacity for discovery has zoomed to the nanoscale, fluorescence microscopy has struggled to keep up. Now, a team that includes UGA engineer Peter Kner has developed a microscope that is capable of live imaging at double the resolution of fluorescence microscopy using structured illumination.

The laws of physics have limited the resolution of fluorescence microscopy, whereby a fluorescent marker is used to distinguish specific proteins, to about 200 nanometers. At this resolution significant detail is lost about the activity within a cell. The increased resolution by structured illumination is an engineering feat that will help scientists learn more about cell behavior and study mechanisms important for human disease.

"Our understanding of what is going on inside cells and our ability to manipulate them has advanced so much that it has become more and more important to see them at a better resolution," said Kner, who joined UGA this spring semester. Kner built the structured illumination microscope with colleagues at the University of California, San Francisco.

This work follows on at least a decade of research building on the nearly fifty-year history of fluorescence microscopy. The technology has been a multi-disciplinary springboard of optical engineering, chemistry and biology, in which the disciplines have all contributed to visualizing fluorescent dyes attached to proteins, advancing our understanding of cellular activity. The importance of fluorescence microscopy was recently recognized with the 2008 Nobel Prize for Chemistry which was awarded for the development of the green fluorescent protein (GFP), which has played a crucial role in our identification and understanding of proteins.

"What we've done is develop a much faster system that allows you to look at live cells expressing GFP, which is a very powerful tool for labeling inside the cell," Kner explained.

"It would be difficult to overstate the importance of bio-imaging to ongoing research in human health," said Dale Threadgill, director of the UGA Faculty of Engineering. "The ability to shine a light on the leading-edge of scientific discovery will define the route to entirely new regimens of health management at the intersections of science and engineering, and Dr. Kner has joined a distinguished cadre at UGA to continue working at that interface," Threadgill added.


Story Source:

The above story is based on materials provided by University of Georgia. Note: Materials may be edited for content and length.


Journal Reference:

  1. Kner et al. Super-resolution video microscopy of live cells by structured illumination. Nature Methods, 2009; 6 (5): 339 DOI: 10.1038/nmeth.1324

Cite This Page:

University of Georgia. "Microscope Capable Of Live Imaging At Double The Resolution Of Fluorescence Microscopy Developed." ScienceDaily. ScienceDaily, 11 May 2009. <www.sciencedaily.com/releases/2009/05/090504161706.htm>.
University of Georgia. (2009, May 11). Microscope Capable Of Live Imaging At Double The Resolution Of Fluorescence Microscopy Developed. ScienceDaily. Retrieved August 21, 2014 from www.sciencedaily.com/releases/2009/05/090504161706.htm
University of Georgia. "Microscope Capable Of Live Imaging At Double The Resolution Of Fluorescence Microscopy Developed." ScienceDaily. www.sciencedaily.com/releases/2009/05/090504161706.htm (accessed August 21, 2014).

Share This




More Matter & Energy News

Thursday, August 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Flower Power! Dandelions Make Car Tires?

Flower Power! Dandelions Make Car Tires?

Reuters - Business Video Online (Aug. 20, 2014) Forget rolling on rubber, could car drivers soon be traveling on tires made from dandelions? Teams of scientists are racing to breed a type of the yellow flower whose taproot has a milky fluid with tire-grade rubber particles in it. As Joanna Partridge reports, global tire makers are investing millions in research into a new tire source. Video provided by Reuters
Powered by NewsLook.com
Awesome New Camouflage Sheet Was Inspired By Octopus Skin

Awesome New Camouflage Sheet Was Inspired By Octopus Skin

Newsy (Aug. 19, 2014) Scientists have developed a new device that mimics the way octopuses blend in with their surroundings to hide from dangerous predators. Video provided by Newsy
Powered by NewsLook.com
Researcher Testing on-Field Concussion Scanners

Researcher Testing on-Field Concussion Scanners

AP (Aug. 19, 2014) Four Texas high school football programs are trying out an experimental system designed to diagnose concussions on the field. The technology is in response to growing concern over head trauma in America's most watched sport. (Aug. 19) Video provided by AP
Powered by NewsLook.com
Green Power Blooms as Japan Unveils 'hydrangea Solar Cell'

Green Power Blooms as Japan Unveils 'hydrangea Solar Cell'

AFP (Aug. 19, 2014) A solar cell that resembles a flower is offering a new take on green energy in Japan, where one scientist is searching for renewables that look good. Duration: 01:29 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins