Featured Research

from universities, journals, and other organizations

Mutations directly identifiable in active genes

Date:
April 19, 2010
Source:
Uppsala University
Summary:
Researchers have developed a new method for identifying genetic variation, including mutations, in active genes. Hopes are strong that the method represents an important research tool that will lead to the development of new diagnostic tests.

Researchers at Uppsala University have developed a new method for identifying genetic variation, including mutations, in active genes. Hopes are strong that the method represents an important research tool that will lead to the development of new diagnostic tests.

The new method, which is directly applicable to cell preparations and tissue sections, should enable studies of the effects of genetic variation in patient samples from a variety of diseases, including, particularly, cancer. The method was developed under the supervision of Mats Nilsson, Professor of Molecular Diagnostics at the Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University. The findings have just been published as an article in the journal Nature Methods.

The method is an elaboration of a technique previously developed by the same research team, involving the use of molecular "padlock probes" to identify specific molecules in individual cells. The probes are able to to distinguish similar genetic sequences, which makes them highly suitable for mutation analysis. Due to the signal amplification associated with padlock probes it is, for the first time, possible to directly identify genetic variation at the mRNA level, that is to say, in molecules produced by active genes, in cells in microscopic preparations, .

"The method allows us to study biological processes in individual cells as opposed to the average states of large numbers of cells," says Mats Nilsson.

Processes specific to cells that represent a minority in the context of a given sample can thus be identified, since their associated signals can escape being drowned out by those generated by the majority. The method is thus of significant interest to the study of tumour tissue, which contains a mix of cancer and normal cells.

"Hitting the proverbial needle in the haystack should now be possible," says Mats Nilsson. "This should entail significantly more sensitive and precise diagnostic methods, improving the prospects that patients will receive the treatment they need."

The method should also make possible various ways of studying the effects of genetic variants on different types of cells and tissues, something that is difficult with methods that rely on preparations comprising a multitude of tissue cells.

The researchers aim to extend the method to allow for parallel identification of multiple molecules and for analysis of biobank material.


Story Source:

The above story is based on materials provided by Uppsala University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Larsson et al. In situ detection and genotyping of individual mRNA molecules. Nature Methods, 2010; DOI: 10.1038/nmeth.1448

Cite This Page:

Uppsala University. "Mutations directly identifiable in active genes." ScienceDaily. ScienceDaily, 19 April 2010. <www.sciencedaily.com/releases/2010/04/100412100013.htm>.
Uppsala University. (2010, April 19). Mutations directly identifiable in active genes. ScienceDaily. Retrieved July 30, 2014 from www.sciencedaily.com/releases/2010/04/100412100013.htm
Uppsala University. "Mutations directly identifiable in active genes." ScienceDaily. www.sciencedaily.com/releases/2010/04/100412100013.htm (accessed July 30, 2014).

Share This




More Plants & Animals News

Wednesday, July 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Raw: Thousands Flocking to German Crop Circle

Raw: Thousands Flocking to German Crop Circle

AP (July 30, 2014) Thousands of people are trekking to a Bavarian farmer's field to check out a mysterious set of crop circles. (July 30) Video provided by AP
Powered by NewsLook.com
Concern Grows Over Worsening Ebola Crisis

Concern Grows Over Worsening Ebola Crisis

AFP (July 30, 2014) Pan-African airline ASKY has suspended all flights to and from the capitals of Liberia and Sierra Leone amid the worsening Ebola health crisis, which has so far caused 672 deaths in Guinea, Liberia and Sierra Leone. Duration: 00:43 Video provided by AFP
Powered by NewsLook.com
At Least 20 Chikungunya Cases in New Jersey

At Least 20 Chikungunya Cases in New Jersey

AP (July 30, 2014) At least 20 New Jersey residents have tested positive for chikungunya, a mosquito-borne virus that has spread through the Caribbean. (July 30) Video provided by AP
Powered by NewsLook.com
Raw: Otters Enjoy Water Slides at Japan Zoo

Raw: Otters Enjoy Water Slides at Japan Zoo

AP (July 30, 2014) River otters were hitting the water slides to beat the summer heatwave on Wednesday at Ichikawa City's Zoological and Botanical Garden. (July 30) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



      Save/Print:
      Share:

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile: iPhone Android Web
      Follow: Facebook Twitter Google+
      Subscribe: RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins