Featured Research

from universities, journals, and other organizations

New retrieval method makes studying cancer proteins easier

Date:
July 11, 2010
Source:
Purdue University
Summary:
Researchers can now better retrieve specific proteins needed to study how cancer cells form by using a newly developed technique and synthetic nanopolymer.

A Purdue University researcher can better retrieve specific proteins needed to study how cancer cells form by using a newly developed technique and synthetic nanopolymer.

Related Articles


W. Andy Tao, an assistant professor of biochemistry, said these specific proteins, called phosphoproteins, can be mapped and analyzed so that we can find ways to inhibit the processes that lead to cancer. But first those few proteins must be fished out of a sea of thousands of others.

Tao developed and patented the polymer-based metal-ion affinity capture, or PolyMAC. The synthetic nanopolymer isolates proteins and peptides that have undergone a process called phosphorylation that is highly associated with cancer, and a patented technique allows Tao to retrieve those proteins. Obtaining the information on these proteins is important for studying how to inhibit the processes that lead to cancer.

"You really want to capture these particular proteins, but there are so many different types of proteins around them," said Tao, whose findings were published in the early online version of the journal Molecular & Cellular Proteomics. "The target proteins are a thousand times lower in amount than other proteins. They are difficult to study without the capturing step."

Normal cells grow, divide and eventually die. But cancer cells continue to grow and do not die. Tao said phosphorylation -- in which a type of enzyme called a kinase attaches to and catalyzes a protein on a cell -- is thought in many cases to be responsible for creating cancer cells.

Tao's nanopolymer is water-soluble and has titanium ions on its surface, which bind with phosphorylated proteins and peptides contained in a solution. The polymer also has a chemical group attached that is reactive and attached to small beads, which allow Tao to retrieve the polymers.

"Once you put the nanopolymer in the solution, you have to retrieve them, so we put a handle on the polymer so we can grab on to it and fish it out of the solution," Tao said.

In laboratory tests, Tao's nanopolymer and retrieval technique isolated about twice as many proteins that had been phosphorylated by an enzyme highly expressed in certain leukemia cells but absent in metastatic breast cancer cells.

Tao is now seeking opportunities to get the polymer and technique into wider use to aid in the development of new cancer drugs.

"This technique is very useful and can be used widely in research for cancer as well as infectious diseases," Tao said.

The National Institutes of Health and the National Science Foundation funded Tao's research. A $1 million NIH grant under the American Reinvestment and Recovery Act paid for a mass spectrometer Tao uses to analyze and map the proteins he recovers using his nanopolymer and retrieval technique.


Story Source:

The above story is based on materials provided by Purdue University. The original article was written by Brian Wallheimer. Note: Materials may be edited for content and length.


Journal Reference:

  1. Iliuk et al. In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion functionalized soluble nanopolymers. Molecular & Cellular Proteomics, 2010; DOI: 10.1074/mcp.M110.000091

Cite This Page:

Purdue University. "New retrieval method makes studying cancer proteins easier." ScienceDaily. ScienceDaily, 11 July 2010. <www.sciencedaily.com/releases/2010/07/100707152223.htm>.
Purdue University. (2010, July 11). New retrieval method makes studying cancer proteins easier. ScienceDaily. Retrieved October 31, 2014 from www.sciencedaily.com/releases/2010/07/100707152223.htm
Purdue University. "New retrieval method makes studying cancer proteins easier." ScienceDaily. www.sciencedaily.com/releases/2010/07/100707152223.htm (accessed October 31, 2014).

Share This



More Health & Medicine News

Friday, October 31, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

U.S. Ebola Response Measures Demonstrated

U.S. Ebola Response Measures Demonstrated

AP (Oct. 31, 2014) Officials in the Washington area showed off Ebola response measures being taken at Dulles International Airport and the National Institutes of Health. (Oct. 31) Video provided by AP
Powered by NewsLook.com
Fauci Says Ebola Risk in US "essentially Zero"

Fauci Says Ebola Risk in US "essentially Zero"

AP (Oct. 30, 2014) NIAID Director Anthony Fauci said the risk of Ebola becoming an epidemic in the U.S. is essentially zero Thursday at the Washington Ideas Forum. He also said an Ebola vaccine will be tested in West Africa in the next few months. (Oct. 30) Video provided by AP
Powered by NewsLook.com
Nurse Defies Ebola Quarantine With Bike Ride

Nurse Defies Ebola Quarantine With Bike Ride

AP (Oct. 30, 2014) A nurse who vowed to defy Maine's voluntary quarantine for health care workers who treated Ebola patients followed through on her promise Thursday, leaving her home for an hour-long bike ride. (Oct. 30) Video provided by AP
Powered by NewsLook.com
Pot-Infused Edibles Raise Concerns in Colorado

Pot-Infused Edibles Raise Concerns in Colorado

AFP (Oct. 30, 2014) Colorado may have legalized marijuana for recreational use, but the debate around the decision still continues, with a recent - failed - attempt to ban cannabis-infused edibles. Duration: 01:53 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins