Featured Research

from universities, journals, and other organizations

Breakthrough towards lab-on-chip system for fast detection of single nucleotide variations in DNA

Date:
December 27, 2010
Source:
Interuniversity Microelectronics Centre (IMEC)
Summary:
Scientists have developed critical components of a biomedical lab-on-chip sensor enabling fast detection of Single Nucleotide Polymorphisms (SNPs) in DNA, such as a miniaturized pump for on-chip generation of high pressures, a micropillar filter optimized for DNA separation achieving world-record resolution, and a SNP detector allowing on-chip detection using very small sample volumes.

Panasonic, core partner within imec's Human++ program and imec has present at the International Electron Devices Meeting in San Francisco various critical components of a biomedical lab-on-chip sensor enabling fast detection of Single Nucleotide Polymorphisms (SNPs) in DNA, such as a miniaturized pump for on-chip generation of high pressures, a micropillar filter optimized for DNA separation achieving world-record resolution, and a SNP detector allowing on-chip detection using very small sample volumes.

A SNP is a single nucleotide replacement in a DNA sequence which can result in different reaction by people to pathogens and medicines. Detection of these SNP's is therefore becoming increasingly important with the move towards more personalized healthcare.

Existing methods to detect SNP's require many sample processing steps in dedicated medical tools at medical laboratories. Such tests are labor intensive, time-consuming and expensive. Moreover, rather large blood samples are needed. A lab-on-chip device can bring huge advantages both to the patient and the healthcare system. Such devices enable fast, easy-to-use, cost-effective test methods which can be performed at regular times in a doctor's office or even near the patient's bed. This is very interesting for point-of-care applications such as personalized medicine.

By combining advanced micro-electronic fabrication processes with heterogeneous integration, imec and Panasonic aim to realize a state-of-the-art microfluidic device for SNP detection. In order to do this, advanced microfluidic components have been fabricated and optimized, such as a miniaturized pump for on-chip generation of high pressure, a micropillar filter optimized for DNA separation achieving world-record resolution, and a SNP detector allowing on-chip detection using very small sample volumes.

The entrance unit of the SNP detection system samples very small volumes of blood. This entrance unit features a miniaturized high-pressure pump based on an advanced conductive polymer actuator. After optimization, the actuator generates high pressures (up to 3MPa) at low voltage (~1.5V). The high pressure is essential to generate a fluid flow through the next unit of the SNP detection system. The on-chip low voltage operation is important because it opens the path to autonomy and portability of the lab-on-chip device.

Next, the DNA separation unit featuring an advanced micro-pillar array filter was developed. This deep-UV patterned silicon pillar array was realized using advanced MEMS technology. It consists of many micron-scale pillars, being typically 20m high and with 1-2m inter-pillar distance. The pillar array is used for DNA separation based on ion-pair reversed-phase (IR-RP) liquid chromatography. Imec and the VUB (Vrije Universiteit Brussel), a scientific partner of imec, optimized the pillar-based IR-RP liquid chromatography technique for DNA separation. This resulted in the first miniaturized on-chip system that enables fast and highly selective separation of short, double stranded DNA strands which only differ 50 base pairs in length. The resolution of the system is the highest in the world and proves the potential to handle 5 SNPs at the same time in the final SNP detection system.

The other functional units of the SNP detector are a unit for DNA extraction and polymerase chain reaction (PCR) using heaters and temperature sensors, and a SNP detection unit based on electrochemical sensors. The miniaturization of these sensors was of crucial importance, since the minimum required sensor volume determines the blood sampling volume needed for the SNP detection, and hence the dimensions of all components of the device. Scientists of Panasonic and imec demonstrated SNP detection capabilities using on-chip sensors handling a volume as small as 0.5L.

Imec's Human++ program provides a multidisciplinary R&D platform for industrial partners to collaborate on finding industry-relevant solutions for future healthcare and wellness needs by combining nanoelectronics and biotechnology into heterogeneous systems for diagnosis and therapy. Human++ partners build on imec's 25 years of expertise in micro- and nanoelectronics and expertise in several healthcare-relevant domains.


Story Source:

The above story is based on materials provided by Interuniversity Microelectronics Centre (IMEC). Note: Materials may be edited for content and length.


Cite This Page:

Interuniversity Microelectronics Centre (IMEC). "Breakthrough towards lab-on-chip system for fast detection of single nucleotide variations in DNA." ScienceDaily. ScienceDaily, 27 December 2010. <www.sciencedaily.com/releases/2010/12/101210075918.htm>.
Interuniversity Microelectronics Centre (IMEC). (2010, December 27). Breakthrough towards lab-on-chip system for fast detection of single nucleotide variations in DNA. ScienceDaily. Retrieved July 24, 2014 from www.sciencedaily.com/releases/2010/12/101210075918.htm
Interuniversity Microelectronics Centre (IMEC). "Breakthrough towards lab-on-chip system for fast detection of single nucleotide variations in DNA." ScienceDaily. www.sciencedaily.com/releases/2010/12/101210075918.htm (accessed July 24, 2014).

Share This




More Matter & Energy News

Thursday, July 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

TSA Administrator on Politics and Flight Bans

TSA Administrator on Politics and Flight Bans

AP (July 24, 2014) TSA administrator, John Pistole's took part in the Aspen Security Forum 2014, where he answered questions on lifting of the ban on flights into Israel's Tel Aviv airport and whether politics played a role in lifting the ban. (July 24) Video provided by AP
Powered by NewsLook.com
Creative Makeovers for Ugly Cellphone Towers

Creative Makeovers for Ugly Cellphone Towers

AP (July 24, 2014) Mobile phone companies and communities across the country are going to new lengths to disguise those unsightly cellphone towers. From a church bell tower to a flagpole, even a pencil, some towers are trying to make a point. (July 24) Video provided by AP
Powered by NewsLook.com
Algonquin Power Goes Activist on Its Target Gas Natural

Algonquin Power Goes Activist on Its Target Gas Natural

TheStreet (July 23, 2014) When The Deal's Amanda Levin exclusively reported that Gas Natural had been talking to potential suitors, the Ohio company responded with a flat denial, claiming its board had not talked to anyone about a possible sale. Lo and behold, Canadian utility Algonquin Power and Utilities not only had approached the company, but it did it three times. Its last offer was for $13 per share as Gas Natural's was trading at a 60-day moving average of about $12.50 per share. Now Algonquin, which has a 4.9% stake in Gas Natural, has taken its case to shareholders, calling on them to back its proposals or, possibly, a change in the target's board. Video provided by TheStreet
Powered by NewsLook.com
Robot Parking Valet Creates Stress-Free Travel

Robot Parking Valet Creates Stress-Free Travel

AP (July 23, 2014) 'Ray' the robotic parking valet at Dusseldorf Airport in Germany lets travelers to avoid the hassle of finding a parking spot before heading to the check-in desk. (July 23) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

    Health News

      Environment News

        Technology News



          Save/Print:
          Share:

          Free Subscriptions


          Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

          Get Social & Mobile


          Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

          Have Feedback?


          Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
          Mobile: iPhone Android Web
          Follow: Facebook Twitter Google+
          Subscribe: RSS Feeds Email Newsletters
          Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins