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Massive endocytosis in cells

Date:
January 17, 2011
Source:
Rockefeller University Press
Summary:
Researchers have extensively characterized a previously unidentified process by which up to 75 percent of the cell plasma membrane can be reversibly endocytosed.
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In three papers in the January and February issues of the Journal of General Physiology (JGP), Don Hilgemann and colleagues have extensively characterized a previously unidentified process by which up to 75% of the cell plasma membrane can be reversibly endocytosed.

This massive endocytosis ("MEND") can be elicited in a variety of cell types with a range of different experimental manipulations, including internal calcium transients in the presence of ATP, membrane treatment with sphingomyelinase, and introduction of various amphiphiles into the membrane bilayer.

MEND does not employ the canonical endocytic mechanisms involving clathrin, the actin cytoskeleton or dynamins. MEND preferentially causes endocytosis of the low-ordered, cholesterol-containing membrane fraction. The mechanisms underlying MEND likely include the merger of nanoscopic low-ordered domains into larger domains with attendant changes in lipid line tension.

Application of MEND promises to serve as a valuable tool in determining which membrane proteins are associated with low- or high-ordered membrane fractions.


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The above post is reprinted from materials provided by Rockefeller University Press. Note: Materials may be edited for content and length.


Journal References:

  1. M. Fine, M. C. Llaguno, V. Lariccia, M.-J. Lin, A. Yaradanakul, D. W. Hilgemann. Massive endocytosis driven by lipidic forces originating in the outer plasmalemmal monolayer: a new approach to membrane recycling and lipid domains. The Journal of General Physiology, 2011; DOI: 10.1085/jgp.201010469
  2. D. W. Hilgemann, M. Fine. Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains. The Journal of General Physiology, 2011; DOI: 10.1085/jgp.201010470
  3. V. Lariccia, M. Fine, S. Magi, M.-J. Lin, A. Yaradanakul, M. C. Llaguno, D. W. Hilgemann. Massive calcium-activated endocytosis without involvement of classical endocytic proteins. The Journal of General Physiology, 2010; 137 (1): 111 DOI: 10.1085/jgp.201010468

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Rockefeller University Press. "Massive endocytosis in cells." ScienceDaily. ScienceDaily, 17 January 2011. <www.sciencedaily.com/releases/2011/01/110117143206.htm>.
Rockefeller University Press. (2011, January 17). Massive endocytosis in cells. ScienceDaily. Retrieved August 4, 2015 from www.sciencedaily.com/releases/2011/01/110117143206.htm
Rockefeller University Press. "Massive endocytosis in cells." ScienceDaily. www.sciencedaily.com/releases/2011/01/110117143206.htm (accessed August 4, 2015).

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