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X‑rays shed new light on regulation of muscle contraction

Date:
April 19, 2011
Source:
European Synchrotron Radiation Facility
Summary:
More than 200 years ago, Luigi Galvani discovered that the muscles of a frog's leg twitch when a voltage is applied. Scientists from Italy, the UK and France have brought this textbook classic into the era of nanoscience. They used a new synchrotron X-ray technique to observe for the first time at the molecular scale how muscle proteins change form and structure inside an intact and contracting muscle cell.

This is the set-up of the experiment at the ESRF where a powerful synchrotron X-ray technique was used to observe for the first time at the molecular scale how muscle proteins change form and structure inside an intact and contracting muscle cell.
Credit: V. Lombardi, Florence University

In a famous experiment first performed more than 220 years ago, Italian physician Luigi Galvani discovered that the muscles of a frog's leg twitch when an electric voltage is applied. An international group of scientists from Italy, the UK and France has now brought this textbook classic into the era of nanoscience. They used a powerful new synchrotron X-ray technique to observe for the first time at the molecular scale how muscle proteins change form and structure inside an intact and contracting muscle cell.

The results are published in the 11 April 2011 issue of the Proceedings of the National Academy of Sciences (PNAS).

The team included scientists from Universitΰ di Firenze (Italy), King's College London (UK) and the European Synchrotron Radiation Facility (ESRF) in Grenoble (France).

A muscle cell contains two sets of filaments composed of the proteins actin and myosin, respectively. Muscles contract as a result of the relative sliding of these filaments. When the brain sends a nerve signal to activate a muscle, the electrical signal is transmitted to the muscle cell. This sets off a chain of events inside the muscle cell that eventually leads to changes in the structure of the myosin and actin filaments.

But what exactly happens during this process at the molecular level?. "As we need muscles for locomotion, breathing and body posture, and for the contraction of the heart, understanding of these mechanisms has broad significance in biology and medicine," says Malcolm Irving from King's College London.

"Studying a single intact and contracting muscle cell at the molecular scale in milliseconds became possible thanks to a new technique called X-ray interferometry based on low angle diffraction. This requires the extremely intense and narrow beam of X-rays provided by the ESRF," adds Theyencheri Narayanan from the ESRF, a co-author of the paper.

The results of the experiment reveal the conformation of the head domains of myosin -- the molecular motors that drive filament sliding -- in resting muscle, and show that the movements of the myosin motors following muscle activation are much slower than the structural changes in the actin filaments. The different timings of the structural changes reveal the signalling pathway between the actin and myosin filaments in muscle, shedding new light on the mechanism of muscle regulation.

"We were observing quite rapid biological processes, in the order of milliseconds, along with minuscule structural changes, typically 10 nm or less. A human hair is ten-thousand times thicker. To integrate the mechanical and X-ray diffraction methods that are required to resolve the combination of these extremes is a real experimental challenge," explains Vincenzo Lombardi from the Unversity of Florence.

Although the study has no immediate clinical application, its longer-term impact may be in the area of heart disease, in which these fundamental signalling mechanisms are not working optimally in the heart muscle. Existing drugs to treat heart failure by modulating this signalling pathway are far from ideal. "To develop better drugs for heart failure, it's likely that a better understanding of the molecular mechanisms of muscle regulation is needed, and that may be the long-term contribution of our experiments," concludes Malcolm Irving.


Story Source:

The above story is based on materials provided by European Synchrotron Radiation Facility. Note: Materials may be edited for content and length.


Journal Reference:

  1. Massimo Reconditi, Elisabetta Brunello, Marco Linari, Pasquale Bianco, Theyencheri Narayanan, Pierre Panine, Gabriella Piazzesi, Vincenzo Lombardi, Malcolm Irving. Motion of myosin head domains during activation and force development in skeletal muscle. Proceedings of the National Academy of Sciences, 2011; DOI: 10.1073/pnas.1018330108

Cite This Page:

European Synchrotron Radiation Facility. "X‑rays shed new light on regulation of muscle contraction." ScienceDaily. ScienceDaily, 19 April 2011. <www.sciencedaily.com/releases/2011/04/110411152524.htm>.
European Synchrotron Radiation Facility. (2011, April 19). X‑rays shed new light on regulation of muscle contraction. ScienceDaily. Retrieved October 21, 2014 from www.sciencedaily.com/releases/2011/04/110411152524.htm
European Synchrotron Radiation Facility. "X‑rays shed new light on regulation of muscle contraction." ScienceDaily. www.sciencedaily.com/releases/2011/04/110411152524.htm (accessed October 21, 2014).

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