Featured Research

from universities, journals, and other organizations

Receptor for Ebola virus identified

Date:
May 3, 2011
Source:
University of Iowa Health Care
Summary:
Researchers have identified a cellular protein that acts as a receptor for Ebola virus and Marburg virus. Furthermore, the team showed that an antibody, which binds to the receptor protein, is able to block infection by both viruses. The results suggest that a human-compatible version of the antibody might provide a way to prevent initial infection during an outbreak.

Cellular protein TIM-1 acts as a receptor for Ebola virus and Marburg virus. Microscope image shows TIM-1 expression (in green) on the surface of human airway cells.
Credit: University of Iowa

A team of researchers has identified a cellular protein that acts as a receptor for Ebola virus and Marburg virus. Furthermore, the team showed that an antibody, which binds to the receptor protein, is able to block infection by both viruses.

"This is the first receptor identified for Ebola and Marburg viruses," said Wendy Maury, Ph.D., associate professor of microbiology at the University of Iowa Roy J. and Lucille A. Carver College of Medicine and senior study author. "That's important because if you can identify and understand the first step in infection -- how the virus enters cells -- then perhaps you can prevent the infection by nipping it in the bud."

Ebola and Marburg viruses cause hemorrhagic fever in humans and other primates. For some strains, infection can lead to death in 50 to 90 percent of cases, and there is no cure or effective treatment. The findings are published online the week of May 2 in the Proceedings of the National Academy of Sciences Early Edition.

Maury led a multidisciplinary team that included colleagues from four UI departments as well as collaborators at the National Institute of Dental and Craniofacial Research (NIDCR) in Bethesda, Md., University of Texas Medical Branch in Galveston, Texas, and Biogen Idec, in Cambridge, Mass.

The researchers used a new bioinformatics-based approach, developed by John Chiorini at NIDCR, to identify a protein called TIM-1 as a receptor for Ebola and Marburg viruses. Subsequent experiments proved that both Ebola and Marburg viruses use TIM-1 is a receptor for infecting cells.

The study also showed that TIM-1 protein is widely expressed on epithelial cells that line various tissues in the body including mucosal surfaces of the airways and in the eyes.

Maury noted that these locations are consistent with some of the ways the Ebola virus is thought to be transmitted -- inhalation of aerosolized droplets and hand-to-eye contact.

A further collaboration with Paul Rennert, Ph.D., at Biogen Idec, a biotech company based in Cambridge, Mass., provided the team with antibodies targeted to TIM-1 and the team found that one of these antibodies, ARD5, very effectively blocks Ebola and Marburg virus entry into cells.

Finally, work performed by Robert Davey, Ph.D., in a BSL-4 lab (the highest level of biocontainment) at University of Texas Medical Branch verified that the ARD5 antibody blocks infection by infectious Zaire Ebola Virus in cells that express the TIM-1 protein.

The results suggest that being able to block Ebola's entry into epithelial cells, perhaps with a human-compatible version of the ARD5 antibody, might provide a way to prevent initial infection and potentially limit the spread of the disease during an outbreak.

Importantly, the study found that TIM-1 protein is not expressed on all the cell types that are infected by Ebola and Marburg.

"It's clear that there are other receptors for Ebola because while TIM-1 is found on a number of epithelial cells in the body, it is not found on some important cell types that are infected by Ebola," Maury said. "Ultimately, epithelial cells are not as important a target for the virus as some other cell types, but they may be the first entry point for Ebola, so they may provide a conduit that allows Ebola access to those other cells within the body."

The research team also included first author Andrew Kondratowicz, a UI graduate student, and UI researchers Paul McCray; Nicholas Lennemann; Patrick Sinn; Catherine Hunt; Sven Moller-Tank; David Meyerholz; Robert Mullins; Melinda Brindley and Lindsay Sanderfeld as well as Kathrina Quinn and Melodie Weller at the NIDCR.

The study was funded in part by grants from the National Institutes of Health.


Story Source:

The above story is based on materials provided by University of Iowa Health Care. Note: Materials may be edited for content and length.


Journal Reference:

  1. Andrew S. Kondratowicz, Nicholas J. Lennemann, Patrick L. Sinn, Robert A. Davey, Catherine L. Hunt, Sven Moller-Tank, David K. Meyerholz, Paul Rennert, Robert F. Mullins, Melinda Brindley, Lindsay M. Sandersfeld, Kathrina Quinn, Melodie Weller, Paul B. Mccray, Jr., John Chiorini, and Wendy Maury. T-cell immunoglobulin and mucin domain 1 (TIM-1) is a receptor for Zaire Ebolavirus and Lake Victoria Marburgvirus. PNAS, May 2, 2011 DOI: 10.1073/pnas.1019030108

Cite This Page:

University of Iowa Health Care. "Receptor for Ebola virus identified." ScienceDaily. ScienceDaily, 3 May 2011. <www.sciencedaily.com/releases/2011/05/110502151349.htm>.
University of Iowa Health Care. (2011, May 3). Receptor for Ebola virus identified. ScienceDaily. Retrieved September 17, 2014 from www.sciencedaily.com/releases/2011/05/110502151349.htm
University of Iowa Health Care. "Receptor for Ebola virus identified." ScienceDaily. www.sciencedaily.com/releases/2011/05/110502151349.htm (accessed September 17, 2014).

Share This



More Health & Medicine News

Wednesday, September 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Artificial Sweetener Could Promote Diabetes

Artificial Sweetener Could Promote Diabetes

Newsy (Sep. 17, 2014) Doctors once thought artificial sweeteners lacked the health risks of sugar, but a new study says they can impact blood sugar levels the same way. Video provided by Newsy
Powered by NewsLook.com
Ebola Vaccine Trial Gets Underway at Oxford University

Ebola Vaccine Trial Gets Underway at Oxford University

AFP (Sep. 17, 2014) A healthy British volunteer is to become the first person to receive a new vaccine for the Ebola virus after US President Barack Obama called for action against the epidemic and warned it was "spiralling out of control." Duration: 01:02 Video provided by AFP
Powered by NewsLook.com
Obesity Rates Steady Even As Americans' Waistlines Expand

Obesity Rates Steady Even As Americans' Waistlines Expand

Newsy (Sep. 17, 2014) Researchers are puzzled as to why obesity rates remain relatively stable as average waistlines continue to expand. Video provided by Newsy
Powered by NewsLook.com
President To Send 3,000 Military Personnel To Fight Ebola

President To Send 3,000 Military Personnel To Fight Ebola

Newsy (Sep. 16, 2014) President Obama is expected to send 3,000 troops to West Africa as part of the effort to contain Ebola's spread. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins