Featured Research

from universities, journals, and other organizations

New fluorescent imaging sorts microbiome in human mouth

Date:
December 9, 2011
Source:
American Society for Cell Biology
Summary:
New fluorescent labeling technology that distinguishes in a single image the population size and spatial distribution of 15 different taxa has uncovered new taxon pairings that indicate unsuspected cooperation -- and standoffishness -- between members of the microbe biofilm that covers teeth.

New fluorescent labeling technology that distinguishes in a single image the population size and spatial distribution of 15 different taxa has uncovered new taxon pairings that indicate unsuspected cooperation -- and standoffishness -- between members of the microbe biofilm that covers teeth, according to a presentation on Dec. 7, at the American Society for Cell Biology's Annual Meeting in Denver.

Related Articles


Members of the genera Prevotella and Actinomyces showed the greatest ability to interact, suggesting a central role for them in producing biofilms, reported the researchers. The study, to determine "who's who" in the human mouth was conducted by researchers at the Marine Biological Laboratory (MBL) in Woods Hole, MA,

While both genera are implicated in periodontal disease, species of Prevotella have been recovered from anaerobic lung infections. Actinomycosis is an infection of antibiotic resistant strains in the mouth and gastrointestinal tract.

Alex Valm, Ph.D., Gary Borisy, Ph.D., and collaborators refer to their new fluorescent labeling technology system as Combinatorial Labeling and Spectral Imaging (CLASI). It was designed to overcome a major limit of existing fluorescent labeling system, whose original green fluorescent protein (GFP) tag occurred in one color (green).

A whole palette of colors is now available to scientists through an ever-expanding array of fluorescent proteins or the addition of glowing molecular add-ons called fluorophores, but keeping track of more than a handful of colors becomes exponentially difficult.

The MBL team's first CLASI system used binary combinations of six fluorophores to perform the first quantitative analysis of a large number of microbes in a biofilm.

With the use of novel linear "unmixing" algorithms, the CLASI system is now being scaled up to look at over 100 differently labeled microbes in each image and to build the first systems-level structural analysis of the entire human oral microbiome.


Story Source:

The above story is based on materials provided by American Society for Cell Biology. Note: Materials may be edited for content and length.


Cite This Page:

American Society for Cell Biology. "New fluorescent imaging sorts microbiome in human mouth." ScienceDaily. ScienceDaily, 9 December 2011. <www.sciencedaily.com/releases/2011/12/111207132633.htm>.
American Society for Cell Biology. (2011, December 9). New fluorescent imaging sorts microbiome in human mouth. ScienceDaily. Retrieved March 27, 2015 from www.sciencedaily.com/releases/2011/12/111207132633.htm
American Society for Cell Biology. "New fluorescent imaging sorts microbiome in human mouth." ScienceDaily. www.sciencedaily.com/releases/2011/12/111207132633.htm (accessed March 27, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, March 27, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Raw: Lioness Has Rare Five-Cub Litter

Raw: Lioness Has Rare Five-Cub Litter

AP (Mar. 27, 2015) — A lioness in Pakistan has given birth to five cubs, twice the usual size of a litter. Queen gave birth to two other cubs just nine months ago. (March 27) Video provided by AP
Powered by NewsLook.com
Jockey Motion Tracking Reveals Racing Prowess

Jockey Motion Tracking Reveals Racing Prowess

Reuters - Innovations Video Online (Mar. 26, 2015) — Using motion tracking technology, researchers from the Royal Veterinary College (RVC) are trying to establish an optimum horse riding style to train junior jockeys, as well as enhance safety, health and well-being of both racehorses and jockeys. Matthew Stock reports. Video provided by Reuters
Powered by NewsLook.com
Bear Cubs Tumble for the Media

Bear Cubs Tumble for the Media

Reuters - Light News Video Online (Mar. 26, 2015) — Two Andean bear cubs are unveiled at the U.S. National Zoo in Washington, D.C. Alicia Powell reports. Video provided by Reuters
Powered by NewsLook.com
Botswana Talks to End Illegal Wildlife Trade

Botswana Talks to End Illegal Wildlife Trade

AFP (Mar. 25, 2015) — Experts are gathering in Botswana to try to end the illegal wildlife trade that is decimating populations of elephants, rhinos and other threatened species. Duration: 01:05 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins