Featured Research

from universities, journals, and other organizations

Diagnostic tool: Polymer film loaded with antibodies can capture tumor cells

Date:
February 24, 2012
Source:
RIKEN
Summary:
The development of polymer film loaded with antibodies that can capture tumor cells shows promise as a diagnostic tool. Cancer cells that break free from a tumor and circulate through the bloodstream spread cancer to other parts of the body. But this process, called metastasis, is extremely difficult to monitor because the circulating tumor cells (CTCs) can account for as few as one in every billion blood cells.

The polymer film forms nanodots: tiny bumps that can be functionalized with antibodies to grab passing cancer cells.
Credit: Copyright : 2011 WILEY-VHC Verlag GmbH & Co.

The development of polymer film loaded with antibodies that can capture tumor cells shows promise as a diagnostic tool. Cancer cells that break free from a tumor and circulate through the bloodstream spread cancer to other parts of the body. But this process, called metastasis, is extremely difficult to monitor because the circulating tumor cells (CTCs) can account for as few as one in every billion blood cells.

Related Articles


Research led by scientists at the RIKEN Advanced Science Institute in Wako, in collaboration with colleagues at the University of California, Los Angeles, and the Institute of Chemistry at the Chinese Academy of Sciences, Beijing, has produced a polymer film that can capture specific CTCs1. With further development, the system could help doctors to diagnose an advancing cancer and assess the effectiveness of treatments.

The researchers used a small electrical voltage to help deposit a conducting polymer film of poly(3,4-ethylenedioxythiophene) (PEDOT) bearing carboxylic acid groups on to a 2-centimeter-square glass base (Fig. 1). The polymer formed nanodots, tiny bumps that measure 100 to 300 nanometers across, depending on the voltage used (1-1.4 V).

Adding a chemical linker to the film allowed it to bind a protein called streptavidin; this protein then joined to an antibody. In turn, the antibody could latch on to an antigen called epithelial cell adhesion molecule (EpCAM), which is produced by most tumor cells. In this way, the film could grab tumor cells from just a few milliliters of a blood sample.

The team tested several types of tumor cells on films with various sizes and densities of nanodots, and used a microscope to observe how well they captured the cells. The most effective film, with nanodots measuring about 230 nanometers across and containing about 8 dots per square micrometer, captured roughly 240 breast-cancer cells per square millimeter of film. In contrast, it caught fewer than 30 cervical cancer cells that do not express EpCAM, proving that the antibody used on the film is highly selective. A smooth PEDOT-carboxylic acid film with the same antibody captured only 50 or so breast cancer cells.

The film's efficiency depends on the size and spacing of the nanodots, and the presence of the capturing antibody. Since these can be easily modified, the same method could be used to make films that sense other types of cells.

The next step is to "further optimize the nanostructures of the conducting polymers and understand in more detail the cell-capturing mechanism," says RIKEN unit leader Hsiao-hua Yu. "We are also currently working on a direct electrical readout of the captured cells, without needing to use a microscope."

The corresponding author for this highlight is based at the Yu Initiative Research Unit, RIKEN Advanced Science Institute


Story Source:

The above story is based on materials provided by RIKEN. Note: Materials may be edited for content and length.


Journal Reference:

  1. Jun Sekine, Shyh-Chyang Luo, Shutao Wang, Bo Zhu, Hsian-Rong Tseng, Hsiao-hua Yu. Functionalized Conducting Polymer Nanodots for Enhanced Cell Capturing: The Synergistic Effect of Capture Agents and Nanostructures. Advanced Materials, 2011; 23 (41): 4788 DOI: 10.1002/adma.201102151

Cite This Page:

RIKEN. "Diagnostic tool: Polymer film loaded with antibodies can capture tumor cells." ScienceDaily. ScienceDaily, 24 February 2012. <www.sciencedaily.com/releases/2012/02/120224152751.htm>.
RIKEN. (2012, February 24). Diagnostic tool: Polymer film loaded with antibodies can capture tumor cells. ScienceDaily. Retrieved December 21, 2014 from www.sciencedaily.com/releases/2012/02/120224152751.htm
RIKEN. "Diagnostic tool: Polymer film loaded with antibodies can capture tumor cells." ScienceDaily. www.sciencedaily.com/releases/2012/02/120224152751.htm (accessed December 21, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Sunday, December 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Touch-Free Smart Phone Empowers Mobility-Impaired

Touch-Free Smart Phone Empowers Mobility-Impaired

Reuters - Innovations Video Online (Dec. 21, 2014) A touch-free phone developed in Israel enables the mobility-impaired to operate smart phones with just a movement of the head. Suzannah Butcher reports. Video provided by Reuters
Powered by NewsLook.com
Earthworms Provide Cancer-Fighting Bacteria

Earthworms Provide Cancer-Fighting Bacteria

Reuters - Innovations Video Online (Dec. 21, 2014) Polish scientists isolate bacteria from earthworm intestines which they say may be used in antibiotics and cancer treatments. Suzannah Butcher reports. Video provided by Reuters
Powered by NewsLook.com
Existing Chemical Compounds Could Revive Failing Antibiotics, Says Danish Scientist

Existing Chemical Compounds Could Revive Failing Antibiotics, Says Danish Scientist

Reuters - Innovations Video Online (Dec. 21, 2014) A team of scientists led by Danish chemist Jorn Christensen says they have isolated two chemical compounds within an existing antipsychotic medication that could be used to help a range of failing antibiotics work against killer bacterial infections, such as Tuberculosis. Jim Drury went to meet him. Video provided by Reuters
Powered by NewsLook.com
Hugging It Out Could Help You Ward Off A Cold

Hugging It Out Could Help You Ward Off A Cold

Newsy (Dec. 21, 2014) Carnegie Mellon researchers found frequent hugs can help people avoid stress-related illnesses. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins