Featured Research

from universities, journals, and other organizations

DNA origami puts a smart lid on solid-state nanopore sensors

Date:
April 19, 2012
Source:
Technische Universitaet Muenchen
Summary:
The latest advance in solid-state nanopore sensors -- devices that are made with standard tools of the semiconductor industry yet can offer single-molecule sensitivity for label-free protein screening -- expands their bag of tricks through bionanotechnology. Researchers have enhanced the capabilities of solid-state nanopores by fitting them with cover plates made of DNA. These nanoscale cover plates, with central apertures tailored to various "gatekeeper" functions, are formed by so-called DNA origami techniques.

Origami nanopore.
Credit: Image courtesy of Technische Universitaet Muenchen

The latest advance in solid-state nanopore sensors – devices that are made with standard tools of the semiconductor industry yet can offer single-molecule sensitivity for label-free protein screening – expands their bag of tricks through bionanotechnology. Researchers at the Technische Universitδt Mόnchen have enhanced the capabilities of solid-state nanopores by fitting them with cover plates made of DNA.

The results are published in Angewandte Chemie International Edition.

The nanoscale cover plates, with central apertures tailored to various "gatekeeper" functions, are formed by so-called DNA origami – the art of programming strands of DNA to fold into custom-designed structures with specified chemical properties. Over the past few years, Prof. Hendrik Dietz's research group at TUM has been refining control over DNA origami techniques and demonstrating how structures made in this way can enable scientific investigations in diverse fields. Meanwhile, Dr. Ulrich Rant's research group has been doing the same for solid-state nanopore sensors, where the basic working principle is to urge biomolecules of interest, one at a time, through a nanometer-scale hole in a thin slab of semiconductor material. When biomolecules pass through or linger in such a sensor, minute changes in electrical current flowing through the nanopore translate into information about their identity and physical properties. Now Dietz and Rant, who are both Fellows of the TUM Institute for Advanced Study, have begun to explore what these two technologies can accomplish together.

The new device concept – purely hypothetical before this series of experiments – begins with the placement of a DNA origami "nanoplate" over the narrow end of a conically tapered solid-state nanopore. "Tuning" the size of the central aperture in the DNA nanoplate should allow filtering of molecules by size. A further refinement, placing single-stranded DNA receptors in the aperture as "bait," should allow sequence-specific detection of "prey" molecules. Conceivable applications include biomolecular interaction screens and detection of DNA sequences. In principle, such a device could even serve as the basis of a novel DNA sequencing system.

Step by step, the researchers investigated each of these ideas. They were able to confirm the self-assembly of custom-designed DNA origami nanoplates, and then their placement – after being electrically guided into position – over solid-state nanopores. They were able to demonstrate both size-based filtering of biomolecules and bait/prey detection of specific target molecules. "We're especially excited about the selective potential of the bait/prey approach to single-molecule sensing," Dietz says, "because many different chemical components beyond DNA could be attached to the appropriate site on a DNA nanoplate."

High-resolution sensing applications such as DNA sequencing will face some additional hurdles, however, as Rant explains: "By design, the nanopores and their DNA origami gatekeepers allow small ions to pass through. For some conceivable applications, that becomes an unwanted leakage current that would have to be reduced, along with the magnitude of current fluctuations." “Future work will need to address fundamental questions, such as how the transport of ions across the DNA origami nanoplates affects the measurement accuracy, and how the nanoplates can be anchored more stably on top of the solid-state nanopores.”

This research was supported by the German Excellence Initiative through the TUM Institute for Advanced Study, the Nano Initiative Munich, and the Center for Integrated Protein Science Munich; by the Collaborative Research Center SFB 863 of the German Research Foundation (DFG); and by a European Research Council Starting Grant to Hendrik Dietz. Ruoshan Wei was supported by the TUM Graduate School's Faculty Graduate Center of Physics.



Story Source:

The above story is based on materials provided by Technische Universitaet Muenchen. Note: Materials may be edited for content and length.


Journal Reference:

  1. Ruoshan Wei, Thomas G. Martin, Ulrich Rant, Hendrik Dietz. DNA Origami Gatekeepers for Solid-State Nanopores. Angewandte Chemie International Edition, 2012; DOI: 10.1002/anie.201200688

Cite This Page:

Technische Universitaet Muenchen. "DNA origami puts a smart lid on solid-state nanopore sensors." ScienceDaily. ScienceDaily, 19 April 2012. <www.sciencedaily.com/releases/2012/04/120419091036.htm>.
Technische Universitaet Muenchen. (2012, April 19). DNA origami puts a smart lid on solid-state nanopore sensors. ScienceDaily. Retrieved August 1, 2014 from www.sciencedaily.com/releases/2012/04/120419091036.htm
Technische Universitaet Muenchen. "DNA origami puts a smart lid on solid-state nanopore sensors." ScienceDaily. www.sciencedaily.com/releases/2012/04/120419091036.htm (accessed August 1, 2014).

Share This




More Matter & Energy News

Friday, August 1, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Tesla, Panasonic Ink Deal To Make Huge Battery 'Gigafactory'

Tesla, Panasonic Ink Deal To Make Huge Battery 'Gigafactory'

Newsy (July 31, 2014) — The deal will help build a massive battery factory that Tesla says will produce 500,000 lithium batteries by 2020. Video provided by Newsy
Powered by NewsLook.com
Britain Testing Driverless Cars on Roadways

Britain Testing Driverless Cars on Roadways

AP (July 30, 2014) — British officials said on Wednesday that driverless cars will be tested on roads in as many as three cities in a trial program set to begin in January. Officials said the tests will last up to three years. (July 30) Video provided by AP
Powered by NewsLook.com
7 Ways to Use Toothpaste: Howdini Hacks

7 Ways to Use Toothpaste: Howdini Hacks

Howdini (July 30, 2014) — Fresh breath and clean teeth are great, but have you ever thought, "my toothpaste could be doing more". Well, it can! Lots of things! Howdini has 7 new uses for this household staple. Video provided by Howdini
Powered by NewsLook.com
Smoked: 2015 Ducati Diavel Vs 2014 Chevy Corvette Stingray Drag Race

Smoked: 2015 Ducati Diavel Vs 2014 Chevy Corvette Stingray Drag Race

Cycle World (July 30, 2014) — The Bonnier Motorcycle Group presents Smoked; a three part video series. In this episode the 2015 Ducati Diavel takes on the 2014 Chevy Corvette Stingray Video provided by Cycle World
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins