Featured Research

from universities, journals, and other organizations

Bioluminescent technology for easy tracking of GMO

Date:
April 29, 2012
Source:
BioMed Central Limited
Summary:
It is important to be able to monitor genetically modified (GM) crops, not only in the field but also during the food processing chain. New research shows that products from genetically modified crops can be identified at low concentration, using bioluminescent real time reporter (BART) technology and loop mediated isothermal amplification (LAMP). The combination of these techniques was able to recognize 0.1% GM contamination of maize, far below the current EU limit of 0.9%.

It is important to be able to monitor genetically modified (GM) crops, not only in the field but also during the food processing chain. New research published in BioMed Central's open access journal BMC Biotechnology shows that products from genetically modified crops can be identified at low concentration, using bioluminescent real time reporter (BART) technology and loop mediated isothermal amplification (LAMP). The combination of these techniques was able to recognise 0.1% GM contamination of maize, far below the current EU limit of 0.9%.

Related Articles


In agriculture GM crops have been bred to improve crop yield or viability. For example some are resistant to herbicides or viruses. They are also used in the pharmaceutical industry to produce proteins such as collagen. However there is a constant debate about the safety of these crops and whether the human-made transgenes might enter the wild population by cross-fertilization.and produce herbicide resistant weeds.

Careful handling and sampling techniques are required to assess the GM content of a crop. The most common technique is polymerase chain reaction (PCR), however, this involves complex extraction procedures and rapid thermocycling, both of which require specific equipment. To overcome these problems researchers from Lumora Ltd. assessed whether they could use LAMP to amplify DNA at a constant temperature and use BART to identify GM-specific DNA in real time.

Dr Guy Kiddle from Lumora, who led the research, explained that LAMP-BART was able to detect as little as 0.1% GM contamination of maize, and, compared to PCR, was more tolerant of contaminating polysaccharides, meaning that the DNA clean-up process did not need to be as thorough. He commented, "This method requires only basic equipment for DNA extraction, and a constant temperature for DNA amplification and detection. Consequently LAMP-BART provides a 'field-ready' solution for monitoring GM crops and their interaction with wild plants or non-GM crops."


Story Source:

The above story is based on materials provided by BioMed Central Limited. Note: Materials may be edited for content and length.


Journal Reference:

  1. Guy Kiddle, Patrick Hardinge, Neil Buttigieg, Olga Gandelman, Clint Pereira, Cathal J McElgunn, Manuela Rizzoli, Rebecca Jackson, Nigel Appleton, Cathy Moore, Laurence C. Tisi and James A.H. Murray. GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use. BMC Biotechnology, (in press/April 2012) [link]

Cite This Page:

BioMed Central Limited. "Bioluminescent technology for easy tracking of GMO." ScienceDaily. ScienceDaily, 29 April 2012. <www.sciencedaily.com/releases/2012/04/120429234637.htm>.
BioMed Central Limited. (2012, April 29). Bioluminescent technology for easy tracking of GMO. ScienceDaily. Retrieved March 27, 2015 from www.sciencedaily.com/releases/2012/04/120429234637.htm
BioMed Central Limited. "Bioluminescent technology for easy tracking of GMO." ScienceDaily. www.sciencedaily.com/releases/2012/04/120429234637.htm (accessed March 27, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, March 27, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Raw: Lioness Has Rare Five-Cub Litter

Raw: Lioness Has Rare Five-Cub Litter

AP (Mar. 27, 2015) — A lioness in Pakistan has given birth to five cubs, twice the usual size of a litter. Queen gave birth to two other cubs just nine months ago. (March 27) Video provided by AP
Powered by NewsLook.com
Jockey Motion Tracking Reveals Racing Prowess

Jockey Motion Tracking Reveals Racing Prowess

Reuters - Innovations Video Online (Mar. 26, 2015) — Using motion tracking technology, researchers from the Royal Veterinary College (RVC) are trying to establish an optimum horse riding style to train junior jockeys, as well as enhance safety, health and well-being of both racehorses and jockeys. Matthew Stock reports. Video provided by Reuters
Powered by NewsLook.com
Bear Cubs Tumble for the Media

Bear Cubs Tumble for the Media

Reuters - Light News Video Online (Mar. 26, 2015) — Two Andean bear cubs are unveiled at the U.S. National Zoo in Washington, D.C. Alicia Powell reports. Video provided by Reuters
Powered by NewsLook.com
Botswana Talks to End Illegal Wildlife Trade

Botswana Talks to End Illegal Wildlife Trade

AFP (Mar. 25, 2015) — Experts are gathering in Botswana to try to end the illegal wildlife trade that is decimating populations of elephants, rhinos and other threatened species. Duration: 01:05 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins