Featured Research

from universities, journals, and other organizations

Virus 'barcodes' offer rapid detection of mutated strains

Date:
May 14, 2012
Source:
University of Leeds
Summary:
Researchers are developing a way to 'barcode' viral diseases to rapidly test new outbreaks for potentially lethal mutations.

Researchers at the University of Leeds are developing a way to 'barcode' viral diseases to rapidly test new outbreaks for potentially lethal mutations.

Dr Julian Hiscox and Dr John Barr of the University's Faculty of Biological Sciences are working with the Health Protection Agency Porton (HPA) to build a bank of molecular signatures that will help identify the severity of virus infection from characteristic changes seen in cells. Currently the team is barcoding different strains of influenza virus and human respiratory syncytial virus (HRSV) -- a virus associated with the onset of asthma in young children.

"Diseases such as flu infect and hijack our cells, turning them into virus producing factories," says Dr Hiscox. "The infection causes the balance of proteins in a cell to change -- some proteins are overproduced and others suppressed. Which proteins are affected and by how much varies depending on the type of virus, allowing us to identify a unique barcode of disease for each."

The research, published May 14 in Proteomics, investigates changes in lung cells infected with swine flu from the 2009 outbreak compared with seasonal flu. The team used a labelling technique called SILAC to measure and compare thousands of different proteins in a sample.

This technique was used alongside mass spectrometry to identify the proteins most affected by viral infection and used these as molecular signatures to provide the 'barcode' of disease. The paper reports how several processes in the cell were affected by the virus, with most changes seen in proteins involved in cell replication.

"Swine flu affects the lungs in a similar way to seasonal flu and this was reflected in the barcodes we found for each," explains Dr Barr. "Using this test might have been a way to identify how lethal the 2009 swine flu pandemic was going to be, lessening worldwide panic.

"Our next step is to test more lethal strains of flu, such as bird flu, to see how the barcodes differ. Flu virus frequently mutates, resulting in new strains which may be life-threatening and become pandemic. If we can test new strains using our method, we can determine their potential impact on health by comparing their barcode of disease to those of viruses already studied."

The group from Leeds has already barcoded two types of HRSV which can cause severe respiratory disease in young children. Co-author Professor Miles Carroll of HPA Porton says: "We have focused our work on common respiratory viruses, such as flu and HRSV, but this method could be applied to a wide variety of viruses, including tropical diseases that are prone to sudden outbreaks and can be lethal."

The research was funded by the National Institute for Health Research (NIHR), the Biotechnology and Biological Sciences Research Council (BBSRC) and the Medical Research Council (MRC).


Story Source:

The above story is based on materials provided by University of Leeds. Note: Materials may be edited for content and length.


Journal Reference:

  1. Brian K. Dove, Rebecca Surtees, Thomas J.H. Bean, Diane Munday, Helen M. Wise, Paul Digard, Miles W. Carroll, Paul Ajuh, John N. Barr, Julian A. Hiscox. A quantitative proteomic analysis of lung epithelial (A549) cells infected with 2009 pandemic influenza A virus using stable isotope labelling with amino acids in cell culture. Proteomics, 2012; DOI: 10.1002/pmic.201100470

Cite This Page:

University of Leeds. "Virus 'barcodes' offer rapid detection of mutated strains." ScienceDaily. ScienceDaily, 14 May 2012. <www.sciencedaily.com/releases/2012/05/120514104949.htm>.
University of Leeds. (2012, May 14). Virus 'barcodes' offer rapid detection of mutated strains. ScienceDaily. Retrieved September 17, 2014 from www.sciencedaily.com/releases/2012/05/120514104949.htm
University of Leeds. "Virus 'barcodes' offer rapid detection of mutated strains." ScienceDaily. www.sciencedaily.com/releases/2012/05/120514104949.htm (accessed September 17, 2014).

Share This



More Health & Medicine News

Wednesday, September 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

President To Send 3,000 Military Personnel To Fight Ebola

President To Send 3,000 Military Personnel To Fight Ebola

Newsy (Sep. 16, 2014) President Obama is expected to send 3,000 troops to West Africa as part of the effort to contain Ebola's spread. Video provided by Newsy
Powered by NewsLook.com
Man Floats for 31 Hours in Gulf Waters

Man Floats for 31 Hours in Gulf Waters

AP (Sep. 16, 2014) A Texas man is lucky to be alive after he and three others floated for more than a day in the Gulf of Mexico when their boat sank during a fishing trip. (Sept. 16) Video provided by AP
Powered by NewsLook.com
Ivorians Abandon Monkey Pets in Fear Over Ebola Virus

Ivorians Abandon Monkey Pets in Fear Over Ebola Virus

AFP (Sep. 16, 2014) Since the arrival of Ebola in Ivory Coast, Ivorians have been abandoning their pets, particularly monkeys, in the fear that they may transmit the virus. Duration: 00:47 Video provided by AFP
Powered by NewsLook.com
Study Links Male-Pattern Baldness To Prostate Cancer

Study Links Male-Pattern Baldness To Prostate Cancer

Newsy (Sep. 16, 2014) New findings suggest men with a certain type of baldness at age 45 are 39 percent more likely to develop aggressive prostate cancer. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

      Technology News



      Save/Print:
      Share:

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile: iPhone Android Web
      Follow: Facebook Twitter Google+
      Subscribe: RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins