Featured Research

from universities, journals, and other organizations

Detection and characterization of norovirus in outbreaks of gastroenteritis

Date:
September 12, 2012
Source:
Universiti Teknologi MARA (UiTM)
Summary:
Researchers conducted a study on norovirus (NoV) in lettuces. The virus causes outbreaks of Gastroenteritis among children below age 5 in Malaysia.

University Teknologi MARA researchers conducted a study on norovirus (NoV) in lettuces. The virus causes outbreaks of gastroenteritis among children below age 5 in Malaysia.

Despite improved sanitation, water supplies, and food safety, diarrhea remains a common cause of illness worldwide. It is reported to account for approximately 1.8 million annual deaths in children <5 years of age (Bryce et al., 2005), making it quite necessary to explore etiological agents for diarrheal diseases.

Norovirus (NoV) is an important cause of acute gastroenteritis in children and adults and is transmitted by fecal-oral route.

Universiti Teknologi MARA researchers tested the lettuces for the presence of NoV, which were then examined by immunochromatography and reverse-transcription polymerase chain reaction techniques.

Stool samples were obtained from eight different hospitals in Peninsular Malaysia. Out of 168 stool samples examined, 45.8% were from children less than 5 years old and 22% were antigen positive. Due to small amount of fecal material left from this age bracket, NoV was not screened since it is already known that 90% in this age group are often infected with rotavirus (RV), which is the most common cause of severe diarrhea in children.

From the remaining total screened, only 89% were screened for NoV and 9.3% were NoV genogroup II. By phylogenetic analysis, GII/3 and GII/4 were found to be the prevalent strains. Majority of these samples were closely related to GII/4 Nagano 2007/JP strains from Japan and one was closely related to GII/4 from Bristol, UK.

This study highlighted that the prevalence of NoV GII.4 strains (71.4%) were in concordance to other reports worldwide, with RV (22%) in the children <5 years old and NoV in 9.3% of the cases. In the lettuces tested , NoV antigen was not detected but by amplification of NoV gene, two (10%) were identified as GII.6 strain.

This study also provides valuable information on NoV genotype , which could be used for surveillance and vaccine strategy. The presence of both GII.4 and GII.3 are in concordance to other strains reported elsewhere in the world. This novel GII.4 variant, termed Hunter virus (Bull et al., 2006) have been identified as the etiological agent in 18 outbreaks investigated including The Netherlands, Japan and Taiwan in 2004 and 2005.

This study is the first report of NoV in lettuces in Malaysia. This preliminary study should be continued since NoV is now being identified as one of the common cause of diarrhea and is still under reported in Malaysia.


Story Source:

The above story is based on materials provided by Universiti Teknologi MARA (UiTM). Note: Materials may be edited for content and length.


Cite This Page:

Universiti Teknologi MARA (UiTM). "Detection and characterization of norovirus in outbreaks of gastroenteritis." ScienceDaily. ScienceDaily, 12 September 2012. <www.sciencedaily.com/releases/2012/09/120912093021.htm>.
Universiti Teknologi MARA (UiTM). (2012, September 12). Detection and characterization of norovirus in outbreaks of gastroenteritis. ScienceDaily. Retrieved April 19, 2014 from www.sciencedaily.com/releases/2012/09/120912093021.htm
Universiti Teknologi MARA (UiTM). "Detection and characterization of norovirus in outbreaks of gastroenteritis." ScienceDaily. www.sciencedaily.com/releases/2012/09/120912093021.htm (accessed April 19, 2014).

Share This



More Health & Medicine News

Saturday, April 19, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

'Holy Grail' Of Weight Loss? New Find Could Be It

'Holy Grail' Of Weight Loss? New Find Could Be It

Newsy (Apr. 18, 2014) In a potential breakthrough for future obesity treatments, scientists have used MRI scans to pinpoint brown fat in a living adult for the first time. Video provided by Newsy
Powered by NewsLook.com
Little Progress Made In Fighting Food Poisoning, CDC Says

Little Progress Made In Fighting Food Poisoning, CDC Says

Newsy (Apr. 18, 2014) A new report shows rates of two foodborne infections increased in the U.S. in recent years, while salmonella actually dropped 9 percent. Video provided by Newsy
Powered by NewsLook.com
Scientists Create Stem Cells From Adult Skin Cells

Scientists Create Stem Cells From Adult Skin Cells

Newsy (Apr. 17, 2014) The breakthrough could mean a cure for some serious diseases and even the possibility of human cloning, but it's all still a way off. Video provided by Newsy
Powered by NewsLook.com
Obama: 8 Million Healthcare Signups

Obama: 8 Million Healthcare Signups

AP (Apr. 17, 2014) President Barack Obama gave a briefing Thursday announcing 8 million people have signed up under the Affordable Care Act. He blasted continued Republican efforts to repeal the law. (April 17) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins