Featured Research

from universities, journals, and other organizations

Geometry plays a role in GPCR transmembrane signaling

Date:
September 26, 2012
Source:
Rockefeller University Press
Summary:
A recent study characterizes the movement of rhodopsin, a GPCR and member of a large family of transmembrane receptors responsible for many cellular responses and involved in many human diseases.

In a recent JGP article, researchers developed a novel approach to study GPCR movement within a living cell. This image shows the grid used for modeling diffusion, with tracings from the image of a rod outer segment indicating disc perimeter (white) and incisures (orange).
Credit: Najafi, M., et al. 2012. J. Gen. Physiol. 140:249-266.

A recent study in The Journal of General Physiology characterizes the movement of rhodopsin, a GPCR and member of a large family of transmembrane receptors responsible for many cellular responses and involved in many human diseases.

Related Articles


In living Xenopus rod photoreceptor cells, rhodopsin is found in stacks of disc membranes that are difficult to study due to their small size. Now, Peter Calvert (SUNY Upstate Medical University) and colleagues have developed an approach with two-photon microscopy that, for the first time, allows direct examination within the living cell of how the geometry of the micro-compartment will affect its signaling.

The team focused on the effect of micro-compartments formed by "incisures," radial membrane boundaries that segment the discs into smaller regions. These micro-compartments, defined by the rim and incisures, have a surface area similar to that of a human or mouse rod photoreceptor disc, so that the total number of components in the GPCR signaling cascade are conserved, and the downstream effects of a captured photon of light are similar.

Calvert and colleagues used a novel high-resolution approach to monitor fluorescently labeled rhodopsin as it diffused in the disc membrane. The team developed a model of molecular diffusion to interpret the results that explicitly took the measured micro-compartments into account, and found that the boundary geometry, not heterogeneity in diffusion or a "bound fraction," was sufficient to explain differences in the extent of diffusion or rhodopsin in different regions of the disc.


Story Source:

The above story is based on materials provided by Rockefeller University Press. Note: Materials may be edited for content and length.


Journal Reference:

  1. M. Najafi, M. Haeri, B. E. Knox, W. E. Schiesser, P. D. Calvert. Impact of signaling microcompartment geometry on GPCR dynamics in live retinal photoreceptors. The Journal of General Physiology, 2012; 140 (3): 249 DOI: 10.1085/jgp.201210818

Cite This Page:

Rockefeller University Press. "Geometry plays a role in GPCR transmembrane signaling." ScienceDaily. ScienceDaily, 26 September 2012. <www.sciencedaily.com/releases/2012/09/120926123811.htm>.
Rockefeller University Press. (2012, September 26). Geometry plays a role in GPCR transmembrane signaling. ScienceDaily. Retrieved November 26, 2014 from www.sciencedaily.com/releases/2012/09/120926123811.htm
Rockefeller University Press. "Geometry plays a role in GPCR transmembrane signaling." ScienceDaily. www.sciencedaily.com/releases/2012/09/120926123811.htm (accessed November 26, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Wednesday, November 26, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Pet Dogs to Be Used in Anti-Ageing Trial

Pet Dogs to Be Used in Anti-Ageing Trial

Reuters - Innovations Video Online (Nov. 26, 2014) Researchers in the United States are preparing to discover whether a drug commonly used in human organ transplants can extend the lifespan and health quality of pet dogs. Video provided by Reuters
Powered by NewsLook.com
From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

Newsy (Nov. 25, 2014) The US FDA is announcing new calorie rules on Tuesday that will require everywhere from theaters to vending machines to include calorie counts. Video provided by Newsy
Powered by NewsLook.com
Daily Serving Of Yogurt Could Reduce Risk Of Type 2 Diabetes

Daily Serving Of Yogurt Could Reduce Risk Of Type 2 Diabetes

Newsy (Nov. 25, 2014) Need another reason to eat yogurt every day? Researchers now say it could reduce a person's risk of developing type 2 diabetes. Video provided by Newsy
Powered by NewsLook.com
Madagascar Working to Contain Plague Outbreak

Madagascar Working to Contain Plague Outbreak

AFP (Nov. 24, 2014) Madagascar said Monday it is trying to contain an outbreak of plague -- similar to the Black Death that swept Medieval Europe -- that has killed 40 people and is spreading to the capital Antananarivo. Duration: 00:42 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins