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New insights into functionality of cystic fibrosis protein

Date:
September 26, 2012
Source:
Rockefeller University Press
Summary:
CFTR is an important protein that, when mutated, causes the life-threatening genetic disease cystic fibrosis. A new study details how an accidental discovery has provided new understanding about CFTR functionality.
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A new JGP study provided evidence about the functionality of CFTR, a protein that plays a critical role in cystic fibrosis. Here, an updated model illustrates the relationship between an opening/closing cycle of the gate and ATP consumption in CFTR’s nucleotide-binding domains.
Credit: Jih, K.-Y., et al. 2012. J. Gen. Physiol. 140:347–359.

CFTR is an important protein that, when mutated, causes the life-threatening genetic disease cystic fibrosis. A study in The Journal of General Physiology (JGP) details how an accidental discovery has provided new understanding about CFTR functionality.

From a scientific standpoint, CFTR is unique in that it is the only known ion channel -- a protein pore that enables the passive diffusion of ions across cell membranes -- in the enormous superfamily of ABC proteins, which normally operate as active transporters. As active transporters, ABC proteins use energy derived from ATP hydrolysis to move substrates across the cell membrane against a concentration gradient. Although CFTR is equipped with the same structural elements as that of its ABC family "brethren," it has been unclear whether the ion channel also functions in the same way.

In the October 2012 issue of JGP, Tzyh-Chang Hwang (University of Missouri-Columbia) and colleagues effectively demonstrate that the mechanism through which CFTR functions is indeed akin to that of the ABC transporters. Specifically, the team used a mutant CFTR channel that exhibits two different open states to determine that ATP hydrolysis underlies the unidirectional cycling of CFTR through its open and closed states. This insight provides new evidence about the functionality of a protein that plays an important role in a very prevalent human disease, and continues to be of great interest to researchers.


Story Source:

The above post is reprinted from materials provided by Rockefeller University Press. Note: Materials may be edited for content and length.


Journal Reference:

  1. K.-Y. Jih, Y. Sohma, T.-C. Hwang. Nonintegral stoichiometry in CFTR gating revealed by a pore-lining mutation. The Journal of General Physiology, 2012; 140 (4): 347 DOI: 10.1085/jgp.201210834

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Rockefeller University Press. "New insights into functionality of cystic fibrosis protein." ScienceDaily. ScienceDaily, 26 September 2012. <www.sciencedaily.com/releases/2012/09/120926123847.htm>.
Rockefeller University Press. (2012, September 26). New insights into functionality of cystic fibrosis protein. ScienceDaily. Retrieved August 30, 2015 from www.sciencedaily.com/releases/2012/09/120926123847.htm
Rockefeller University Press. "New insights into functionality of cystic fibrosis protein." ScienceDaily. www.sciencedaily.com/releases/2012/09/120926123847.htm (accessed August 30, 2015).

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