Featured Research

from universities, journals, and other organizations

New guideline for DNA sequences could prevent erroneous data

Date:
November 8, 2012
Source:
University of Gothenburg
Summary:
DNA sequence data is an indispensable source of research information in biology. But not all data are reliable. Almost 10% of all fungal DNA sequences are, for example, incorrectly identified to species level. A international team of researchers has therefore prepared a guide to assist the scientific community in the quality control process.

DNA sequence data is an indispensable source of research information in biology. But not all data are reliable. Almost 10% of all fungal DNA sequences are, for example, incorrectly identified to species level. A international team of researchers, with it's core at the University of Gothenburg, Sweden, has therefore prepared a guide to assist the scientific community in the quality control process.

A new scientific study sees the researchers putting together a number of guidelines to help other researchers to ensure a high level of quality among their newly generated DNA sequences.

DNA sequences make it possible to study biological samples and environments at a level of detail that traditional tools, such as microscopes, cannot provide. It is, for example, possible to investigate what species are present in seemingly barren substrates such as soil and seawater. Such studies often reveal an astonishing and hitherto unimagined diversity, and biology has made major advances as the use of DNA-based methods has become more widespread.

But as with many other sources of information, DNA sequences vary in quality and reliability. Several studies have found considerable quality problems in existing DNA sequence databases.

To verify ones DNA Sequence dataset for basic quality and authenticity has thus become an important part of biological research.

"Many researchers perceive quality control as difficult," says Henrik Nilsson at the University of Gothenburg. "There are, quite simply, no guidelines that you can hand out to new or established researchers so that everyone is using the same approach. Which is why there are major differences in how, and to what extent, quality control is carried out in the research community."

Henrik Nilsson is the lead author of a new scientific article on DNA sequence quality which has been published in the open-access journal MycoKeys.

One complication is that the software that is available to carry out parts of the quality control is cumbersome and often requires considerable computer capacity. The research group feels that it is not appropriate to require all biologists to have access to and be able to use such complex computer systems.

This is why they have written an article describing how quality control can be carried out manually without any tools beyond an Internet browser.

The article features a number of principles and observations on DNA sequences at different quality stages. Although the guidelines focus on fungi, where DNA sequences have had a particularly significant impact as a research instrument, they are general and can be used for most genes and groups of organisms.

The guidelines relate to traditional DNA sequencing as it is used in systematics, taxonomy and ecology.

The researchers hope that it will help readers to improve their DNA sequences and so halt the trend of increasing noise in the public DNA sequence databases.


Story Source:

The above story is based on materials provided by University of Gothenburg. Note: Materials may be edited for content and length.


Journal Reference:

  1. Henrik Nilsson, Leho Tedersoo, Kessy Abarenkov, Martin Ryberg, Erik Kristiansson, Martin Hartmann, Conrad Schoch, Johan Nylander, Johannes Bergsten, Teresita Porter, Ari Jumpponen, Parag Vaishampayan, Otso Ovaskainen, Nils Hallenberg, Johan Bengtsson, Martin Eriksson, Karl-Henrik Larsson, Ellen Larsson, Urmas Koeljalg. Five simple guidelines for establishing basic authenticity and reliability of newly generated fungal ITS sequences. MycoKeys, 2012; 4 (0): 37 DOI: 10.3897/mycokeys.4.3606

Cite This Page:

University of Gothenburg. "New guideline for DNA sequences could prevent erroneous data." ScienceDaily. ScienceDaily, 8 November 2012. <www.sciencedaily.com/releases/2012/11/121108073809.htm>.
University of Gothenburg. (2012, November 8). New guideline for DNA sequences could prevent erroneous data. ScienceDaily. Retrieved August 1, 2014 from www.sciencedaily.com/releases/2012/11/121108073809.htm
University of Gothenburg. "New guideline for DNA sequences could prevent erroneous data." ScienceDaily. www.sciencedaily.com/releases/2012/11/121108073809.htm (accessed August 1, 2014).

Share This




More Health & Medicine News

Friday, August 1, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

House Republicans Vote to Sue Obama Over Healthcare Law

House Republicans Vote to Sue Obama Over Healthcare Law

Reuters - US Online Video (July 31, 2014) The Republican-led House of Representatives votes to sue President Obama, accusing him of overstepping his executive authority in making changes to the Affordable Care Act. Mana Rabiee reports. Video provided by Reuters
Powered by NewsLook.com
Despite Health Questions, E-Cigs Are Beneficial: Study

Despite Health Questions, E-Cigs Are Beneficial: Study

Newsy (July 31, 2014) Citing 81 previous studies, new research out of London suggests the benefits of smoking e-cigarettes instead of regular ones outweighs the risks. Video provided by Newsy
Powered by NewsLook.com
Dangerous Bacteria Kills One in Florida

Dangerous Bacteria Kills One in Florida

AP (July 31, 2014) Sarasota County, Florida health officials have issued a warning against eating raw oysters and exposing open wounds to coastal and inland waters after a dangerous bacteria killed one person and made another sick. (July 31) Video provided by AP
Powered by NewsLook.com
Health Insurers' Profits Slide

Health Insurers' Profits Slide

Reuters - Business Video Online (July 30, 2014) Obamacare-related costs were said to be behind the profit plunge at Wellpoint and Humana, but Wellpoint sees the new exchanges boosting its earnings for the full year. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins