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Mice with fluorescent replicating cells could help cancer and regenerative medicine research

Date:
November 29, 2012
Source:
Hebrew University of Jerusalem
Summary:
A newly-engineered strain of mice whose dividing cells express a fluorescent protein could open the door to new methods of regulating cell proliferation in humans. Cell proliferation plays a key role in degenerative diseases, in which specific cells do not replicate enough, and in cancers, in which cells replicate too much.

Fluorescence microscopy section of the intestine. Green/yellow areas mark sites of cell proliferation. Cell nuclei marked in blue and nuclei with DNA replication marked in red.
Credit: Agnes Klochendler

A newly-engineered strain of mice whose dividing cells express a fluorescent protein could open the door to new methods of regulating cell proliferation in humans. Cell proliferation plays a key role in degenerative diseases, in which specific cells do not replicate enough, and in cancers, in which cells replicate too much.

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Cells in the human body grow and multiply during body growth or during tissue regeneration after damage. However most mature tissues require only rare cell divisions. Scientists who wish to study these rare populations of replicating cells face a serious obstacle: most current methods for labeling and identifying replicating cells involve procedures that kill the cells and destroy sensitive biological material. This limits the ability of scientists to examine important functions of these cells, for example the genes active in such cells.

To address this problem, two Hebrew University of Jerusalem researchers -- Prof. Yuval Dor from the Institute for Medical Research Israel-Canada (IMRIC) and Dr. Amir Eden from the Alexander Silberman Institute of Life Sciences -- together with colleagues in Denmark and the U.S., created a mouse strain in which replicating cells express a fluorescent protein which is destroyed once cell division is completed. In all tissues of these mice, replicating cells are labeled by green fluorescence, which allows identification and isolation of live, replicating cells directly from healthy or diseased tissue.

Using this system, research associate Dr. Agnes Klochendler and PhD student Noa Weinberg-Corem at the Hebrew University were able to isolate a rare population of replicating cells from the livers of mice, and study the genes that they express compared with resting liver cells. Interestingly, they found that in replicating liver cells there is a significant decrease in the expression of genes responsible for key liver functions such as fatty acid and amino acid metabolism.

The research results indicate that when differentiated cells divide, they temporarily shift to a less differentiated state. This finding is important to our understanding of the difference between the two fundamental states of differentiation and proliferation in normal cells. It is also relevant for the situation in cancer, where cells are proliferating and often less differentiated.

In the future, the researchers hope to develop methods for regulating cell proliferation. For example, isolation and study of the rare replicating cells in the pancreas could lead to development of approaches to promote the proliferation and expansion of insulin-producing cells, whose loss is the hallmark of diabetes.

This could also be useful in other areas such as cancer and regenerative biology. By distinguishing between abnormally expressed genes in tumors and the genes associated with normal cell divisions, researchers may be able to identify cancer-specific replication markers with a potential to become new drug targets. Similarly, scientists could analyze the effects of specific drugs on the biology of replicating cells, providing important clues for regenerative medicine.


Story Source:

The above story is based on materials provided by Hebrew University of Jerusalem. Note: Materials may be edited for content and length.


Journal Reference:

  1. Agnes Klochendler, Noa Weinberg-Corem, Maya Moran, Avital Swisa, Nathalie Pochet, Virginia Savova, Jonas Vikeså, Yves Van de Peer, Michael Brandeis, Aviv Regev, Finn Cilius Nielsen, Yuval Dor, Amir Eden. A Transgenic Mouse Marking Live Replicating Cells Reveals In Vivo Transcriptional Program of Proliferation. Developmental Cell, 2012; 23 (4): 681 DOI: 10.1016/j.devcel.2012.08.009

Cite This Page:

Hebrew University of Jerusalem. "Mice with fluorescent replicating cells could help cancer and regenerative medicine research." ScienceDaily. ScienceDaily, 29 November 2012. <www.sciencedaily.com/releases/2012/11/121129093333.htm>.
Hebrew University of Jerusalem. (2012, November 29). Mice with fluorescent replicating cells could help cancer and regenerative medicine research. ScienceDaily. Retrieved December 18, 2014 from www.sciencedaily.com/releases/2012/11/121129093333.htm
Hebrew University of Jerusalem. "Mice with fluorescent replicating cells could help cancer and regenerative medicine research." ScienceDaily. www.sciencedaily.com/releases/2012/11/121129093333.htm (accessed December 18, 2014).

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