Featured Research

from universities, journals, and other organizations

Pocket test measures fifty things in a drop of blood

Date:
December 19, 2012
Source:
Methodist Hospital, Houston
Summary:
A new device about the size of a business card could allow health care providers to test for insulin and other blood proteins, cholesterol, and even signs of viral or bacterial infection all at the same time —- with one drop of blood.

Blood from a pin prick can be used to determine the presence and approximate amount of 50 different small molecules, including viral antigens, hormones like insulin, as well as HDL or LDL cholesterols.
Credit: Lidong Qin and Yujun Song

A new device about the size of a business card could allow health care providers to test for insulin and other blood proteins, cholesterol, and even signs of viral or bacterial infection all at the same time -- with one drop of blood. Preliminary tests of the V-chip, created by scientists at The Methodist Hospital Research Institute and MD Anderson Cancer Center, were just published by Nature Communications.

"The V-Chip could make it possible to bring tests to the bedside, remote areas, and other types of point-of-care needs," said Nanomedicine faculty member Lidong Qin, Ph.D., the project's principal investigator. "V-Chip is accurate, cheap, and portable. It requires only a drop of a sample, not a vial of blood, and can do 50 different tests in one go."

Similar assays are typically done using heavy, large, complex equipment such as mass spectrometers, or require fluoroscopy analysis, which must also be done in a lab.

The V-chip, short for "volumetric bar-chart chip," on the other hand, can be carried around in a pocket. It is composed of two thin pieces of glass, about 3 in. by 2 in. In between are wells for four things: (1) hydrogen peroxide, (2) up to 50 different antibodies to specific proteins, DNA or RNA fragments, or lipids of interest, and the enzyme catalase, (3) serum or other sample, and (4) a dye -- any dye will do. Initially, the wells are kept separate from each other. A shift in the glass plates brings the wells into contact, creating a contiguous, zig-zagged space from one end of the V-chip to the other.

As the substance of interest -- say, insulin -- binds to antibodies bound to the glass slide, catalase is made active and splits nearby hydrogen peroxide into water and oxygen gas. This approach is called ELISA, or enzyme-linked immunosorbent assay. The oxygen pushes the dye up the column. The more present insulin is, the more oxygen is created, and the farther dye is pushed up the slide. Tests show that distance is more or less proportional to the amount of substrate present, in this example, insulin. The end result is a visual bar chart. Easy to read and accurate, Qin says, though development continues.

"The sensitivity of the V-chip can be improved if narrower and longer bar channels are used," Qin said. "Our next steps are to make the device more user friendly and be so simple to use, it barely needs instructions."

Qin is also a Weill Cornell Medical College assistant professor of cell and developmental biology.


Story Source:

The above story is based on materials provided by Methodist Hospital, Houston. Note: Materials may be edited for content and length.


Journal Reference:

  1. Yujun Song, Yuanqing Zhang, Paul E. Bernard, James M. Reuben, Naoto T. Ueno, Ralph B. Arlinghaus, Youli Zu, Lidong Qin. Multiplexed volumetric bar-chart chip for point-of-care diagnostics. Nature Communications, 2012; 3: 1283 DOI: 10.1038/ncomms2292

Cite This Page:

Methodist Hospital, Houston. "Pocket test measures fifty things in a drop of blood." ScienceDaily. ScienceDaily, 19 December 2012. <www.sciencedaily.com/releases/2012/12/121219152621.htm>.
Methodist Hospital, Houston. (2012, December 19). Pocket test measures fifty things in a drop of blood. ScienceDaily. Retrieved July 31, 2014 from www.sciencedaily.com/releases/2012/12/121219152621.htm
Methodist Hospital, Houston. "Pocket test measures fifty things in a drop of blood." ScienceDaily. www.sciencedaily.com/releases/2012/12/121219152621.htm (accessed July 31, 2014).

Share This




More Matter & Energy News

Thursday, July 31, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Britain Testing Driverless Cars on Roadways

Britain Testing Driverless Cars on Roadways

AP (July 30, 2014) British officials said on Wednesday that driverless cars will be tested on roads in as many as three cities in a trial program set to begin in January. Officials said the tests will last up to three years. (July 30) Video provided by AP
Powered by NewsLook.com
Amid Drought, UCLA Sees Only Water

Amid Drought, UCLA Sees Only Water

AP (July 30, 2014) A ruptured 93-year-old water main left the UCLA campus awash in 8 million gallons of water in the middle of California's worst drought in decades. (July 30) Video provided by AP
Powered by NewsLook.com
Smartphone Powered Paper Plane Debuts at Airshow

Smartphone Powered Paper Plane Debuts at Airshow

AP (July 30, 2014) Smartphone powered paper airplane that was popular on crowdfunding website KickStarter makes its debut at Wisconsin airshow (July 30) Video provided by AP
Powered by NewsLook.com
U.K. To Allow Driverless Cars On Public Roads

U.K. To Allow Driverless Cars On Public Roads

Newsy (July 30, 2014) Driverless cars could soon become a staple on U.K. city streets, as they're set to be introduced to a few cities in 2015. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



      Save/Print:
      Share:

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile: iPhone Android Web
      Follow: Facebook Twitter Google+
      Subscribe: RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins