Featured Research

from universities, journals, and other organizations

A rapid, paper-based diagnostic test for tuberculosis

Date:
October 3, 2013
Source:
National Institute for Materials Science
Summary:
Researchers describe a simple, color-based diagnostic approach with the potential to detect target DNA sequences found in TB-causing mycobacteria – in just a fraction of the time required for established diagnostic tests.

Schematic of the proposed TB diagnosis. Unknown extracted human DNA sequences are first hybridized with detection oligonucleotide sequences, followed by addition of colloidal gold nanoparticles and triggering of the colorimetric sensing with a sodium chloride solution. If the extracted DNA sequences consist of IS6110 target sequences then the detection oligonucleotide sequences will hybridize with them, and only a few ssDNA sequences will be absorbed on the gold nanoparticles to avoid aggregation after the addition of salt. In the absence of IS6110 target sequences, the color of the mixture remains red after hybridization and does not change. The mixture is then spotted and concentrated on the chromatography paper confined by solid wax hydrophobic barriers. Diagnostic results can be photographed with a smartphone and sent to a server for cloud computing.
Credit: Image courtesy of National Institute for Materials Science

In a study published in the journal Science and Technology of Advanced Materials (STAM), researchers in Taiwan describe a simple, color-based diagnostic approach with the potential to detect target DNA sequences found in TB-causing mycobacteria -- in just a fraction of the time required for established diagnostic tests.

Related Articles


More than a century after the identification of organisms that cause tuberculosis (TB), this disease remains a global public health challenge. According to World Health Organization estimates, there were 8.7 million new cases in 2011 and 1.4 million deaths. Most new cases occur in developing countries that lack the facilities and trained personnel required for early detection of TB.

In a new study, published in the journal Science and Technology of Advanced Materials (STAM), researchers in Taiwan describe a simple, color-based diagnostic approach with the potential to detect target DNA sequences found in TB-causing mycobacteria -- in just a fraction of the time required for established diagnostic tests.

The standard method for TB detection in a clinical setting involves culturing the Mycobacterium tuberculosis bacillus, which requires 3-6 weeks to grow on solid culture media or 9-16 days in rapid liquid culture media. A faster alternative is the polymerase chain reaction (PCR) technology. However, it is still too slow (turnaround time 2-5 hours) and requires sophisticated infrastructure and trained personnel that might be unavailable in developing countries.

In their STAM paper, Tsung-Ting Tsai and colleagues employed gold nanoparticles and microfluidic paper-based analytical devices to achieve rapid diagnosis without the need for complex and time-consuming laboratory processes. They easily detected TB mycobacterium target sequences, and the turnaround time was approximately 1 hour after the human DNA was extracted from patients.

Although the authors are still optimizing their technology, they already believe that it will result in "affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and highly end-user-deliverable diagnostic applications."


Story Source:

The above story is based on materials provided by National Institute for Materials Science. Note: Materials may be edited for content and length.


Journal Reference:

  1. Tsung-Ting Tsai, Shu-Wei Shen, Chao-Min Cheng, Chien-Fu Chen. Paper-based tuberculosis diagnostic devices with colorimetric gold nanoparticles. Science and Technology of Advanced Materials, 2013; 14 (4): 044404 DOI: 10.1088/1468-6996/14/4/044404

Cite This Page:

National Institute for Materials Science. "A rapid, paper-based diagnostic test for tuberculosis." ScienceDaily. ScienceDaily, 3 October 2013. <www.sciencedaily.com/releases/2013/10/131003142216.htm>.
National Institute for Materials Science. (2013, October 3). A rapid, paper-based diagnostic test for tuberculosis. ScienceDaily. Retrieved October 24, 2014 from www.sciencedaily.com/releases/2013/10/131003142216.htm
National Institute for Materials Science. "A rapid, paper-based diagnostic test for tuberculosis." ScienceDaily. www.sciencedaily.com/releases/2013/10/131003142216.htm (accessed October 24, 2014).

Share This



More Health & Medicine News

Friday, October 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

IKEA Desk Converts From Standing to Sitting With One Button

IKEA Desk Converts From Standing to Sitting With One Button

Buzz60 (Oct. 24, 2014) IKEA is out with a new convertible desk that can convert from a sitting desk to a standing one with just the push of a button. Jen Markham explains. Video provided by Buzz60
Powered by NewsLook.com
Ebola Protective Suits Being Made in China

Ebola Protective Suits Being Made in China

AFP (Oct. 24, 2014) A factory in China is busy making Ebola protective suits for healthcare workers and others fighting the spread of the virus. Duration: 00:38 Video provided by AFP
Powered by NewsLook.com
WHO: Millions of Ebola Vaccine Doses by 2015

WHO: Millions of Ebola Vaccine Doses by 2015

AP (Oct. 24, 2014) The World Health Organization said on Friday that millions of doses of two experimental Ebola vaccines could be ready for use in 2015 and five more experimental vaccines would start being tested in March. (Oct. 24) Video provided by AP
Powered by NewsLook.com
Doctor in NYC Quarantined With Ebola

Doctor in NYC Quarantined With Ebola

AP (Oct. 24, 2014) An emergency room doctor who recently returned to the city after treating Ebola patients in West Africa has tested positive for the virus. He's quarantined in a hospital. (Oct. 24) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins