Featured Research

from universities, journals, and other organizations

DNA of peanut-allergic kids changes with immune therapy, study finds

Date:
January 31, 2014
Source:
Stanford University Medical Center
Summary:
Treating a peanut allergy with oral immunotherapy changes the DNA of the patient's immune cells, according to a new study. The DNA change could serve as the basis for a simple blood test to monitor the long-term effectiveness of the allergy therapy.

Research led by immunologist Kari Nadeau shows a blood test could determine whether patients who have been desensitized to their peanut allergies need to continue eating peanuts daily to retain their immunity.
Credit: Steve Fisch

Treating a peanut allergy with oral immunotherapy changes the DNA of the patient's immune cells, according to a new study from the Stanford University School of Medicine and Lucile Packard Children's Hospital Stanford. The DNA change could serve as the basis for a simple blood test to monitor the long-term effectiveness of the allergy therapy.

Peanut allergy, like other food allergies, currently has no cure. Scientists are conducting clinical trials of doctor-supervised immunotherapy, in which peanut-allergic patients take increasing amounts of peanut powder to try to desensitize them to the peanut allergen. At the end of the trial, patients are usually asked to eat some peanuts every day for the rest of their lives.

"At first, eating two peanut butter cups a day might seem fun, but it gets a little boring and a lot of people might stop," said Kari Nadeau, MD, PhD, associate professor of pediatrics at Stanford and an immunologist at Stanford Hospital & Clinics and Lucile Packard Children's Hospital Stanford. Until now, doctors could not test whether patients who had completed immunotherapy could safely stop eating daily doses of peanuts, she said. "Our new finding can help us try to determine whether, for the long term, someone's allergy has truly been shut off so people can eat ad lib."

Nadeau is the senior author of a paper describing the new findings, which will be published online Jan. 31 in the Journal of Allergy and Clinical Immunology.

In the new study, Nadeau's team examined 20 peanut-allergic children and adults who had completed two years of immunotherapy, which enabled them to eat one 4-gram serving of peanuts daily without experiencing a major allergic reaction.

The patients were asked to stop eating peanuts for three months and then were given a small amount of peanut powder to see if their allergy returned. Thirteen of the patients regained their allergy, while seven did not. The researchers compared the immune cells in the blood of patients from the two groups. Blood samples from peanut-allergic patients who had never received oral immunotherapy were used as a control.

The researchers focused on the regulatory T cells, which are white blood cells that help to suppress an allergy response. In these cells, the DNA at a gene called forkhead box protein 3, or FOXP3, was slightly different in each of the three groups of patients. The FOXP3 gene has previously been shown to play a role in allergies.

The researchers did not detect changes in the genetic code itself. Rather, there were different numbers of methyl groups attached to the DNA. These molecular add-ons to the DNA control the rate at which the gene is expressed, turning the volume up or down on the gene's voice in the cell. Patients who maintained their tolerance to peanuts had low levels of DNA methylation at FOXP3, while those who regained their allergy had an intermediate level. Peanut-allergic patients who had not received oral immunotherapy (the control group) had a high level of DNA methylation at the FOXP3 gene.

The DNA change could possibly be used to monitor the effectiveness of oral immunotherapy, Nadeau said, but needs further testing. The blood test necessary to monitor the DNA is inexpensive, requires only a small amount of blood and uses common lab equipment. However, Food and Drug Administration approval would be needed before the test could be used clinically for this purpose.

"It's interesting that the change we saw is at the epigenetic level," Nadeau said, referring to changes in gene activity and expression caused by factors other than DNA sequence. "This might help us tell people if they can safely go off of immunotherapy, or if they need to continue to eat the food every day." The test could also help researchers determine whether some individuals would benefit from longer courses of immunotherapy, she added.

Future studies are needed to determine whether the genetic change to FOXP3 is passed on to the children of people who have completed oral immunotherapy.


Story Source:

The above story is based on materials provided by Stanford University Medical Center. The original article was written by Erin Digitale. Note: Materials may be edited for content and length.


Journal Reference:

  1. Aleena Syed, Marco A. Garcia, Shu-Chen Lyu, Robert Bucayu, Arunima Kohli, Satoru Ishida, Jelena P. Berglund, Mindy Tsai, Holden Maecker, Gerri O’Riordan, Stephen J. Galli, Kari C. Nadeau. Peanut oral immunotherapy results in increased antigen-induced regulatory T-cell function and hypomethylation of forkhead box protein 3 (FOXP3). Journal of Allergy and Clinical Immunology, 2014; 133 (2): 500 DOI: 10.1016/j.jaci.2013.12.1037

Cite This Page:

Stanford University Medical Center. "DNA of peanut-allergic kids changes with immune therapy, study finds." ScienceDaily. ScienceDaily, 31 January 2014. <www.sciencedaily.com/releases/2014/01/140131150219.htm>.
Stanford University Medical Center. (2014, January 31). DNA of peanut-allergic kids changes with immune therapy, study finds. ScienceDaily. Retrieved July 28, 2014 from www.sciencedaily.com/releases/2014/01/140131150219.htm
Stanford University Medical Center. "DNA of peanut-allergic kids changes with immune therapy, study finds." ScienceDaily. www.sciencedaily.com/releases/2014/01/140131150219.htm (accessed July 28, 2014).

Share This




More Health & Medicine News

Monday, July 28, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Trees Could Save More Than 850 Lives Each Year

Trees Could Save More Than 850 Lives Each Year

Newsy (July 27, 2014) A national study conducted by the USDA Forest Service found that trees collectively save more than 850 lives on an annual basis. Video provided by Newsy
Powered by NewsLook.com
Google's Next Frontier: The Human Body

Google's Next Frontier: The Human Body

Newsy (July 27, 2014) Google is collecting genetic and molecular information to paint a picture of the perfectly healthy human. Video provided by Newsy
Powered by NewsLook.com
What's To Blame For Worst Ebola Outbreak In History?

What's To Blame For Worst Ebola Outbreak In History?

Newsy (July 27, 2014) A U.S. doctor has tested positive for the deadly Ebola virus, as the worst-ever outbreak continues to grow. Video provided by Newsy
Powered by NewsLook.com
Losing Sleep Leaves You Vulnerable To 'False Memories'

Losing Sleep Leaves You Vulnerable To 'False Memories'

Newsy (July 27, 2014) A new study shows sleep deprivation can make it harder for people to remember specific details of an event. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins