Featured Research

from universities, journals, and other organizations

Combatting hospital-acquired infections with protein metal complex

Date:
March 6, 2014
Source:
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University
Summary:
A protein containing a metal complex for blue paint inhibits growth of a pathogenic bacterium through iron deprivation. Scientists have found a new method using an artificial metalloprotein (a protein that contains a metal) to inhibit the growth of Pseudomonas aeruginosa bacteria, which is a common bacterium that can cause diseases in humans and evolves to exhibit multiple antibiotic resistance.

Heme iron capturing mechanism of P. aeruginosa bacteria by HasA protein.
Credit: Nagoya University

A protein containing a metal complex for blue paint inhibits growth of a pathogenic bacterium through iron deprivation.

Related Articles


Professor Yoshihito Watanabe (WPI-ITbM, Cooperating Researcher), Associate Professor Osami Shoji, Ms. Chikako Shirataki of Nagoya University and co-workers have found a new method using an artificial metalloprotein (a protein that contains a metal) to inhibit the growth of Pseudomonas aeruginosa bacteria, which is a common bacterium that can cause diseases in humans and evolves to exhibit multiple antibiotic resistance. The inhibition of growth has been achieved through the deprivation of iron uptake using an artificial metalloprotein. The study published in the online Early View on February 7, 2014 of Angewandte Chemie International Edition, is expected to bring hope in the battle against bacteria.

P. aeruginosa bacteria exists in many aquatic areas and is prevalent in hospitals. Although they do not usually affect healthy people, they increase the risk for infection of patients with low immunity. Their high resistance towards many antibiotics makes complete elimination of them extremely difficult. Like humans, bacteria require the uptake of heme iron for their survival, and a protein (HasA) is secreted from bacteria to capture heme from its host. The heme-bound HasA protein transfers heme via receptor proteins on the cell surface of the bacterium, P. aeruginosa.

"Upon looking closely at the crystal structure of the HasA protein binding heme, we considered the possibility of the HasA protein to bind to a metal complex that has a similar structure as heme" says Associate Professor Osami Shoji, who led the study. "We found synthetic metal complexes that can mimic heme and bind to the HasA protein. To our delight, one of the resulting complexes successfully inhibited growth of P. aeruginosa bacteria."

"It took us around four years to discover that phthalocyanine, which is a blue paint used on the surface of the Japanese bullet trains and road signs, could bind competitively to the HasA protein," adds Ms. Chikako Shirataki, a PhD student in her final year, "crystal structures of metal protein complexes helped us to show that the phthalocyanine-bound HasA protein blocks the receptors on the cell surface of the bacterium and thus, inhibits the uptake of heme." When bacteria are deprived of iron, further growth of the bacteria is inhibited.

P. aeruginosa infections can potentially lead to pneumonia and an effective treatment method is highly required. This finding by Shoji's group opens new doors to treat P. aeruginosa infections by using an unprecedented approach to inhibit the growth of bacteria. Associate Professor Shoji states, "With the advice of medical doctors, we are currently working to develop a new system to wipe out bacteria by tuning various metal complexes. Although the efficiency is not high yet, we have already established a mechanism to eliminate bacteria and we are considering how to apply it to different cases."


Story Source:

The above story is based on materials provided by Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Chikako Shirataki, Osami Shoji, Mitsuyoshi Terada, Shin-ichi Ozaki, Hiroshi Sugimoto, Yoshitsugu Shiro, Yoshihito Watanabe. Inhibition of Heme Uptake inPseudomonas aeruginosaby its Hemophore (HasAp) Bound to Synthetic Metal Complexes. Angewandte Chemie International Edition, 2014; DOI: 10.1002/anie.201307889

Cite This Page:

Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University. "Combatting hospital-acquired infections with protein metal complex." ScienceDaily. ScienceDaily, 6 March 2014. <www.sciencedaily.com/releases/2014/03/140306132811.htm>.
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University. (2014, March 6). Combatting hospital-acquired infections with protein metal complex. ScienceDaily. Retrieved December 21, 2014 from www.sciencedaily.com/releases/2014/03/140306132811.htm
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University. "Combatting hospital-acquired infections with protein metal complex." ScienceDaily. www.sciencedaily.com/releases/2014/03/140306132811.htm (accessed December 21, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Sunday, December 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Researchers Test Colombian Village With High Alzheimer's Rates

Researchers Test Colombian Village With High Alzheimer's Rates

AFP (Dec. 19, 2014) In Yarumal, a village in N. Colombia, Alzheimer's has ravaged a disproportionately large number of families. A genetic "curse" that may pave the way for research on how to treat the disease that claims a new victim every four seconds. Duration: 02:42 Video provided by AFP
Powered by NewsLook.com
Monarch Butterflies Descend Upon Mexican Forest During Annual Migration

Monarch Butterflies Descend Upon Mexican Forest During Annual Migration

Reuters - Light News Video Online (Dec. 19, 2014) Millions of monarch butterflies begin to descend onto Mexico as part of their annual migration south. Rough Cut (no reporter narration) Video provided by Reuters
Powered by NewsLook.com
The Best Protein-Filled Foods to Energize You for the New Year

The Best Protein-Filled Foods to Energize You for the New Year

Buzz60 (Dec. 19, 2014) The new year is coming and nothing will energize you more for 2015 than protein-filled foods. Fitness and nutrition expert John Basedow (@JohnBasedow) gives his favorite high protein foods that will help you build muscle, lose fat and have endless energy. Video provided by Buzz60
Powered by NewsLook.com
Birds Might Be Better Meteorologists Than Us

Birds Might Be Better Meteorologists Than Us

Newsy (Dec. 19, 2014) A new study suggests a certain type of bird was able to sense a tornado outbreak that moved through the U.S. a day before it hit. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins