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Enzyme 'wrench' could be key to stronger, more effective antibiotics

Date:
April 10, 2014
Source:
North Carolina State University
Summary:
Builders and factory workers know that getting a job done right requires precision and specialized tools. The same is true when you're building antibiotic compounds at the molecular level. New findings may turn an enzyme that acts as a specialized 'wrench' in antibiotic assembly into a set of wrenches that will allow for greater customization.

Builders and factory workers know that getting a job done right requires precision and specialized tools. The same is true when you're building antibiotic compounds at the molecular level. New findings from North Carolina State University may turn an enzyme that acts as a specialized "wrench" in antibiotic assembly into a set of wrenches that will allow for greater customization. By modifying this enzyme, scientists hope to be able to design and synthesize stronger, more adaptable antibiotics from less expensive, natural compounds.

Kirromycin is a commonly known antibiotic that can be created through natural synthesis; that is, it doesn't have to be made in a chemistry lab. Nature creates compounds like kirromycin through a factory-like assembly line of enzymes where each performs a specific function, snapping different fragments of molecules together like a jigsaw puzzle. Understanding this process on the molecular level could give chemists the ability to piggyback on nature, synthesizing new antibiotics and cancer drugs with less waste and expense.

NC State chemist Gavin Williams looked at one enzyme in the kirromycin assembly line -- KirCII -- which is responsible for installing a molecular fragment of kirromycin at one key location. "KirCII is a linchpin enzyme in the assembly," Williams says. "Natural compounds like kirromycin get built in pieces, with small modules, or blocks of enzymes, linking up sections of the compound in a certain order. Enzymes like KirCII are the wrenches that install the molecular pieces -- without them, the molecule doesn't finish assembling properly."

Williams and his team performed a molecular analysis of KirCII to determine why and how it latches onto a specific protein within the kirromycin assembly line. They saw that the enzyme has electrical charges on its surface that are complementary to opposite charges on the surface of the protein it binds with. When KirCII finds that protein, the charges match up and it snaps into place.

"We were able to see which areas on KirCII had charges that worked with the target protein," Williams says. "Hopefully we will be able to use this information to introduce complementary charges onto the surface of other proteins we want KirCII to bind with.

"Right now KirCII is just one wrench. By modifying it to fit other proteins, we could turn it into a set of different wrenches and create totally different antibiotics. Kirromycin isn't very useful right now, but by using KirCII to install pieces from other antibiotics, we'll be able to mix and match and create new, stronger antibiotics."


Story Source:

The above story is based on materials provided by North Carolina State University. The original article was written by Tracey Peake. Note: Materials may be edited for content and length.


Journal Reference:

  1. Zhixia Ye, Ewa M. Musiol, Tilmann Weber, Gavin J. Williams. Reprogramming Acyl Carrier Protein Interactions of an Acyl-CoA Promiscuous trans-Acyltransferase. Chemistry and Biology, April 2014 DOI: 10.1016/j.chembiol.2014.02.019

Cite This Page:

North Carolina State University. "Enzyme 'wrench' could be key to stronger, more effective antibiotics." ScienceDaily. ScienceDaily, 10 April 2014. <www.sciencedaily.com/releases/2014/04/140410122159.htm>.
North Carolina State University. (2014, April 10). Enzyme 'wrench' could be key to stronger, more effective antibiotics. ScienceDaily. Retrieved September 16, 2014 from www.sciencedaily.com/releases/2014/04/140410122159.htm
North Carolina State University. "Enzyme 'wrench' could be key to stronger, more effective antibiotics." ScienceDaily. www.sciencedaily.com/releases/2014/04/140410122159.htm (accessed September 16, 2014).

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