Featured Research

from universities, journals, and other organizations

New method isolates immune cells to study how they ward off oral diseases

Date:
April 15, 2014
Source:
Case Western Reserve University
Summary:
Dental researchers have found a less invasive way to extract single rare immune cells from the mouth to study how the mouth’s natural defenses ward off infection and inflammation. By isolating some specialized immune cells (white blood cells known as "leukocytes") to study how they fight diseases in the mouth -- or reject foreign tissues, such as in failed organ transplants -- researchers hope to learn more about treating and preventing such health issues as oral cancers, cardiovascular disease, AIDS and other infectious diseases.

Case Western Reserve University dental researchers have found a less invasive way to extract single rare immune cells from the mouth to study how the mouth's natural defenses ward off infection and inflammation.

By isolating some specialized immune cells (white blood cells known as "leukocytes") to study how they fight diseases in the mouth -- or reject foreign tissues, such as in failed organ transplants -- researchers hope to learn more about treating and preventing such health issues as oral cancers, cardiovascular disease, AIDS and other infectious diseases.

To this point, researchers have had to rely on studying and growing immune cells from blood. Studying tissue immune cells allows researchers to learn how they function at the site of infection.

The role of adaptive immune cells in the stomach and intestines is more widely known, yet the role of similar cells in the mouth is unclear. There are no reliable methods to extract immune cells from mouth, which are more accessible and easier to extract than harder-to-reach tissues in the stomach and intestines.

But, until now, immune cells removed from the mouth couldn't be isolated with enough viability or grown to study their activities, Pushpa Pandiyan, assistant professor of biological sciences at the dental school, explained.

The new method, developed by Pandiyan, the study's lead author, is described in Biological Procedures article, "Isolation of T cells from mouse oral tissues."

Pandiyan, who studies oral diseases associated with HIV, found no reliable method existed to isolate and keep a single cell from the tongue, gums and palate alive long enough to study.

Pandiyan and her team developed a way to do so successfully. The researchers reported that more than 94 percent of the isolated cells lived long enough to study.

Their method

Using mouse models, the researchers isolated two important specialized immune T lymphocytes that play a role in fighting oral diseases. The cells are part of the adaptive immune system in which cells respond to pathogens invading the body.

The researchers took tissue samples from the mouths of mice and washed them several times in saline and chemical solutions with antibiotics. This was followed by disintegrating the tissue using salts and enzymes. The solution was centrifuged and strained to separate different tissue parts with more washings and separations before the cells could be studied and grown.


Story Source:

The above story is based on materials provided by Case Western Reserve University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Pushpa Pandiyan, Natarajan Bhaskaran, Yifan Zhang, Aaron Weinberg. Isolation of T cells from mouse oral tissues. Biological Procedures Online, 2014; 16 (1): 4 DOI: 10.1186/1480-9222-16-4

Cite This Page:

Case Western Reserve University. "New method isolates immune cells to study how they ward off oral diseases." ScienceDaily. ScienceDaily, 15 April 2014. <www.sciencedaily.com/releases/2014/04/140415125305.htm>.
Case Western Reserve University. (2014, April 15). New method isolates immune cells to study how they ward off oral diseases. ScienceDaily. Retrieved July 29, 2014 from www.sciencedaily.com/releases/2014/04/140415125305.htm
Case Western Reserve University. "New method isolates immune cells to study how they ward off oral diseases." ScienceDaily. www.sciencedaily.com/releases/2014/04/140415125305.htm (accessed July 29, 2014).

Share This




More Health & Medicine News

Tuesday, July 29, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Deadly Ebola Virus Threatens West Africa

Deadly Ebola Virus Threatens West Africa

AP (July 28, 2014) West African nations and international health organizations are working to contain the largest Ebola outbreak in history. It's one of the deadliest diseases known to man, but the CDC says it's unlikely to spread in the U.S. (July 28) Video provided by AP
Powered by NewsLook.com
$15B Deal on Vets' Health Care Reached

$15B Deal on Vets' Health Care Reached

AP (July 28, 2014) A bipartisan deal to improve veterans health care would authorize at least $15 billion in emergency spending to fix a veterans program scandalized by long patient wait times and falsified records. (July 28) Video provided by AP
Powered by NewsLook.com
Two Americans Contract Ebola in Liberia

Two Americans Contract Ebola in Liberia

Reuters - US Online Video (July 28, 2014) Two American aid workers in Liberia test positive for Ebola while working to combat the deadliest outbreak of the virus ever. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Traditional African Dishes Teach Healthy Eating

Traditional African Dishes Teach Healthy Eating

AP (July 28, 2014) Classes are being offered nationwide to encourage African Americans to learn about cooking fresh foods based on traditional African cuisine. The program is trying to combat obesity, heart disease and other ailments often linked to diet. (July 28) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins