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MEMS nanoinjector for genetic modification of cells

Date:
May 13, 2014
Source:
American Institute of Physics (AIP)
Summary:
The ability to transfer a gene or DNA sequence from one animal into the genome of another plays a critical role in a wide range of medical research—including cancer, Alzheimer’s disease, and diabetes. But the traditional method of transferring genetic material into a new cell, called "microinjection," has a serious downside. It involves using a small glass pipette to pump a solution containing DNA into the nucleus of an egg cell, but the extra fluid can cause the cell to swell and destroy it -- resulting in a 25 to 40 percent cell death rate.

This SEM (scanning electron microscope) image shows the nanoinjector next to a latex bead the same size as an egg cell. You can see the size of the nanoinjector and its lance compared to a cell.
Credit: Brian Jensen/BYU

The ability to transfer a gene or DNA sequence from one animal into the genome of another plays a critical role in a wide range of medical research -- including cancer, Alzheimer's disease, and diabetes.

But the traditional method of transferring genetic material into a new cell, called "microinjection," has a serious downside. It involves using a small glass pipette to pump a solution containing DNA into the nucleus of an egg cell, but the extra fluid can cause the cell to swell and destroy it -- resulting in a 25 to 40 percent cell death rate.

Now, thanks to the work of researchers from Brigham Young University, there's a way to avoid cell death when introducing DNA into egg cells. In Review of Scientific Instruments, the team describes its microelectromechanical system (MEMS) nanoinjector, which was designed to inject DNA into mouse zygotes (single-cell embryos consisting of a fertilized egg).

"Essentially, we use electrical forces to attract and repel DNA -- allowing injections to occur with a tiny, electrically conductive lance," explained Brian Jensen, associate professor in the Department of Mechanical Engineering at Brigham Young University. "DNA is attracted to the outside of the lance using positive voltage, and then the lance is inserted into a cell."

The MEMS nanoinjector's lance is incredibly small and no extra fluid is used with this technique, so cells undergo much less stress compared to the traditional microinjection process.

This ability to inject DNA into cells without causing cell death leads to "more efficient injections, which in turn reduces the cost to create a transgenic animal," according to Jensen.

One of the team's most significant findings is that it's possible to use the electrical forces to get DNA into the nucleus of the cell -- without having to carefully aim the lance into the pronucleus (the cellular structure containing the cell's DNA). "This may enable future automation of the injections, without requiring manual injection," Jensen says.

It may also mean that injections can be performed in animals with cloudy or opaque embryos. "Such animals, including many interesting larger ones like pigs, would be attractive for a variety of transgenic technologies," said Jensen. "We believe nanoinjection may open new fields of discovery in these animals."

As a next step, Jensen and colleagues are performing injections into cells in a cell culture using an array of lances that can inject hundreds of thousands of cells at once. "We expect the lance array may enable gene therapy using a culture of a patient's own cells," he noted.

The paper's first author Quentin Aten participated in this research while at Brigham Young University. He is now working at Nexus Spine LLC.


Story Source:

The above story is based on materials provided by American Institute of Physics (AIP). Note: Materials may be edited for content and length.


Journal Reference:

  1. Quentin T. Aten, Brian D. Jensen, Sandra H. Burnett, and Larry L. Howell. A Self-Reconfiguring Metamorphic Nanoinjector for Injection into Mouse Zygotes. Review of Scientific Instruments, May 13, 2014 DOI: 10.1063/1.4872077

Cite This Page:

American Institute of Physics (AIP). "MEMS nanoinjector for genetic modification of cells." ScienceDaily. ScienceDaily, 13 May 2014. <www.sciencedaily.com/releases/2014/05/140513113252.htm>.
American Institute of Physics (AIP). (2014, May 13). MEMS nanoinjector for genetic modification of cells. ScienceDaily. Retrieved October 22, 2014 from www.sciencedaily.com/releases/2014/05/140513113252.htm
American Institute of Physics (AIP). "MEMS nanoinjector for genetic modification of cells." ScienceDaily. www.sciencedaily.com/releases/2014/05/140513113252.htm (accessed October 22, 2014).

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