Featured Research

from universities, journals, and other organizations

Identifying cyst-laden meat: Sarcocystis thermostable PCR detection kit developed

Date:
June 12, 2014
Source:
Universiti Teknologi MARA (UiTM)
Summary:
Consumption of undercooked cyst-laden meat from cattle, sheep and goats may cause infection in humans. Researchers have successfully invented a PCR kit which provides a suitable and feasible means of screening, detection and identification with high sensitivity and specificity of the parasite.

Consumption of undercooked cyst-laden meat from cattle, sheep and goats may cause infection in humans. Researchers from Universiti Teknologi MARA have successfully invented a PCR kit which provides a suitable and feasible means of screening, detection and identification with high sensitivity and specificity of the parasite.

Related Articles


Sarcosytis spp are intracellular protozoan parasites acquired upon consumption of undercooked cyst-laden meat from cattle, sheep and goats. Humans and some primates are defective hosts for Sarcosystis hominis and S. suihominis after ingesting raw meat from cattle and pigs, respectively.

Cases of human infection have been documented. Humans carry the intramuscular micro and macrocyst. The actual incidence among humans is yet to be assessed. The asexual stages, including sarcoyst, can provoke a strong inflammatory reaction in the tissues.

Human cases have been reported with acute myositis, diarrhea and fever. Livestock suffer acute debilitating infections, resulting in abortion and death or chronic infections with failure to grow or thrive. It is indeed regrettable that this disease has not received the attention it deserves and remains a neglected one.

Studies have shown that sarcosytis spp were seen in 56% of hamburgers, 20% of hotdogs and sausages. Definitive diagnosis with identification of sporocysts in feces requires multiple stool examinations, several days after having eaten the meat.

Sarcocystis sarcocysts in muscle biopsy specimens can be identified by microscopic examination of histological sections stained with haematoxylin and eosin and other stains such as PAS reaction. Basic histological examinations, by no means, can help definitive identification of sarcocystis at spies level and warrants electron microscopy or molecular analysis.

It has also been reported that polymerase chain reaction PCR positive samples were reported negative on histology. PCR provides a feasible means for screening, detection and identification with high sensitivity and specificity of the parasite.

Our PCR kit has been created with thermo stabilised PCR reactions Premixes consists of buffered salt solution with dNTPs, MgCl and Taq polymerase enzyme. The user just adds templates, primers and PCR grade distilled water and amplify based on the given conditions.

Advantages Amplifies multiple genes simultaneously requires no cold chain built-in gel loading dye which facilitates the loading of PCR products directly onto the agaose gel without addition of sample loading buffer, easy to follow steps minimises handling and pipetting useful for field works fast accurate and affordable PCR provides a suitable and feasible means for screening detection and identification with high sensitivity and specificity of the parasite.

Currently there are no commercially available PCR detection kits for sarcocystis. This kit can be of invaluable help for large scale quick screening of meat for sarcocystis. thus its value cannot be overemphasized for not only meat industry but also the ministry concerned with food and meat safety.


Story Source:

The above story is based on materials provided by Universiti Teknologi MARA (UiTM). Note: Materials may be edited for content and length.


Cite This Page:

Universiti Teknologi MARA (UiTM). "Identifying cyst-laden meat: Sarcocystis thermostable PCR detection kit developed." ScienceDaily. ScienceDaily, 12 June 2014. <www.sciencedaily.com/releases/2014/06/140612212432.htm>.
Universiti Teknologi MARA (UiTM). (2014, June 12). Identifying cyst-laden meat: Sarcocystis thermostable PCR detection kit developed. ScienceDaily. Retrieved March 29, 2015 from www.sciencedaily.com/releases/2014/06/140612212432.htm
Universiti Teknologi MARA (UiTM). "Identifying cyst-laden meat: Sarcocystis thermostable PCR detection kit developed." ScienceDaily. www.sciencedaily.com/releases/2014/06/140612212432.htm (accessed March 29, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Sunday, March 29, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

S. Leone in New Anti-Ebola Lockdown

S. Leone in New Anti-Ebola Lockdown

AFP (Mar. 28, 2015) — Sierra Leone imposed a three-day nationwide lockdown Friday for the second time in six months in a bid to prevent a resurgence of the deadly Ebola virus. Duration: 01:17 Video provided by AFP
Powered by NewsLook.com
These Popular Antibiotics Can Cause Permanent Nerve Damage

These Popular Antibiotics Can Cause Permanent Nerve Damage

Newsy (Mar. 27, 2015) — A popular class of antibiotic can leave patients in severe pain and even result in permanent nerve damage. Video provided by Newsy
Powered by NewsLook.com
WH Plan to Fight Antibiotic-Resistant Germs

WH Plan to Fight Antibiotic-Resistant Germs

AP (Mar. 27, 2015) — The White House on Friday announced a five-year plan to fight the threat posed by antibiotic-resistant bacteria amid fears that once-treatable germs could become deadly. (March 27) Video provided by AP
Powered by NewsLook.com
House Ready to Pass Medicare Doc Bill

House Ready to Pass Medicare Doc Bill

AP (Mar. 26, 2015) — In rare bipartisan harmony, congressional leaders pushed a $214 billion bill permanently blocking physician Medicare cuts toward House passage Thursday, moving lawmakers closer to resolving a problem that has plagued them for years. (March 26) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins