Featured Research

from universities, journals, and other organizations

Ötzi's non-human DNA: Opportunistic pathogen discovered in Iceman tissue biopsy

Date:
July 15, 2014
Source:
European Academy of Bozen/Bolzano
Summary:
Ötzi’s human genome was decoded from a hip bone sample taken from the 5,300 year old mummy. However the tiny sample weighing no more than 0.1 g provides so much more information. A team of scientists analyzed the non-human DNA in the sample. They found evidence for the presence of Treponema denticola, an opportunistic pathogen involved in the development of periodontal disease.

A team of scientists from EURAC in Bolzano/Bozen together with colleagues from the University of Vienna successfully analysed the non-human DNA in the sample.
Credit: Frank Maixner (EURAC)

Ötzi's human genome was decoded from a hip bone sample taken from the 5,300 year old mummy. However the tiny sample weighing no more than 0.1 g provides so much more information. A team of scientists from EURAC in Bolzano/Bozen together with colleagues from the University of Vienna successfully analysed the non-human DNA in the sample. They found evidence for the presence of Treponema denticola, an opportunistic pathogen involved in the development of periodontal disease. Thus, by just looking at the DNA, the researchers could support a CT-based diagnosis made last year which indicated that the Iceman suffered from periodontitis. The results of the current study have recently been published in the online scientific journal PLOS ONE.

Related Articles


Much of what we know about Ötzi -- for example what he looked like or that he suffered from lactose intolerance -- stems from a tiny bone sample which allowed the decoding of his genetic make-up. Now, however, the team of scientists have examined more closely the part of the sample consisting of non-human DNA. "What is new is that we did not carry out a directed DNA analysis but rather investigated the whole spectrum of DNA to better understand which organisms are in this sample and what is their potential function," is how Frank Maixner, from the EURAC Institute for Mummies and the Iceman in Bozen/Bolzano, described the new approach which the team of scientists are now pursuing.

"This 'non-human' DNA mostly derives from bacteria normally living on and within our body. Only the interplay between certain bacteria or an imbalance within this bacterial community might cause certain diseases. Therefore it is highly important to reconstruct and understand the bacterial community composition by analysing this DNA mixture," said Thomas Rattei, Professor of Bioinformatics from the Department of Microbiology and Ecosystem Science at the University of Vienna.

Unexpectedly the team of scientists, specialists in both microbiology as well as bioinformatics, detected in the DNA mixture a sizeable presence of a particular bacterium: Treponema denticola, an opportunistic pathogen involved in the development of periodontitis. Thus this finding supports the computer tomography based diagnosis that the Iceman suffered from periodontitis. Even more surprising is that the analysis of a tiny bone sample can still, after 5,300 years, provide us with the information that this opportunistic pathogen seems to have been distributed via the bloodstream from the mouth to the hip bone. Furthermore, the investigations indicate that these members of the human commensal oral microflora were old bacteria which did not colonise the body after death.

Besides the opportunistic pathogen, the team of scientists led by Albert Zink -- head of the EURAC Institute for Mummies and the Iceman -- also detected Clostridia-like bacteria in the Iceman bone sample which are at present most presumably in a kind of dormant state. Under hermetically sealed, anaerobic conditions, however, these bacteria can re-grow and degrade tissue. This discovery may well play a significant part in the future conservation of the world-famous mummy. "This finding indicates that altered conditions for preserving the glacier mummy, for example when changing to a nitrogen-based atmosphere commonly used for objects of cultural value, will require additional micro-biological monitoring," explained the team of scientists who will now look closer at the microbiome of the Iceman.


Story Source:

The above story is based on materials provided by European Academy of Bozen/Bolzano. Note: Materials may be edited for content and length.


Journal Reference:

  1. Frank Maixner, Anton Thomma, Giovanna Cipollini, Stefanie Widder, Thomas Rattei, Albert Zink. Metagenomic Analysis Reveals Presence of Treponema denticola in a Tissue Biopsy of the Iceman. PLoS ONE, 2014; 9 (6): e99994 DOI: 10.1371/journal.pone.0099994

Cite This Page:

European Academy of Bozen/Bolzano. "Ötzi's non-human DNA: Opportunistic pathogen discovered in Iceman tissue biopsy." ScienceDaily. ScienceDaily, 15 July 2014. <www.sciencedaily.com/releases/2014/07/140715085055.htm>.
European Academy of Bozen/Bolzano. (2014, July 15). Ötzi's non-human DNA: Opportunistic pathogen discovered in Iceman tissue biopsy. ScienceDaily. Retrieved November 28, 2014 from www.sciencedaily.com/releases/2014/07/140715085055.htm
European Academy of Bozen/Bolzano. "Ötzi's non-human DNA: Opportunistic pathogen discovered in Iceman tissue biopsy." ScienceDaily. www.sciencedaily.com/releases/2014/07/140715085055.htm (accessed November 28, 2014).

Share This


More From ScienceDaily



More Fossils & Ruins News

Friday, November 28, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

New Dinosaur Species Found in Museum Collection

New Dinosaur Species Found in Museum Collection

Reuters - Innovations Video Online (Nov. 27, 2014) — A British palaeontologist has discovered a new species of dinosaur while studying fossils in a Canadian museum. Pentaceratops aquilonius was related to Triceratops and lived at the end of the Cretaceous Period, around 75 million years ago. Jim Drury has more. Video provided by Reuters
Powered by NewsLook.com
Classic Hollywood Memorabilia Goes Under the Hammer

Classic Hollywood Memorabilia Goes Under the Hammer

Reuters - Entertainment Video Online (Nov. 26, 2014) — The iconic piano from "Casablanca" and the Cowardly Lion suit from "The Wizard of Oz" fetch millions at auction. Sara Hemrajani reports. Video provided by Reuters
Powered by NewsLook.com
3D Map of Antarctic Sea Ice to Shed Light on Climate Change

3D Map of Antarctic Sea Ice to Shed Light on Climate Change

Reuters - Innovations Video Online (Nov. 24, 2014) — A multinational group of scientists have released the first ever detailed, high-resolution 3-D maps of Antarctic sea ice. Using an underwater robot equipped with sonar, the researchers mapped the underside of a massive area of sea ice to gauge the impact of climate change. Ben Gruber reports. Video provided by Reuters
Powered by NewsLook.com
Ruins Thought To Be Port Actually Buried Greek City

Ruins Thought To Be Port Actually Buried Greek City

Newsy (Nov. 24, 2014) — Media is calling it an "underwater Pompeii." Researchers have found ruins off the coast of Delos. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins