Featured Research

from universities, journals, and other organizations

Novel lung-on-a-chip developed

Date:
August 14, 2014
Source:
RTI International
Summary:
A new lung-on-chip microdevice for laboratory studies of respiratory challenges and therapeutics has been developed by scientists. The microdevice includes multiple vertically stacked cellular layers that mimic the structure of the airway tissue. The cellular model of the airway mucosa could provide insight into biological and pathophysiological effects that conventional cell cultures or animal models do not capture, and help lead to the development of new therapeutics.

RTI’s lung-on-a-chip emulates the multilayer airway tissue microarchitecture. In this picture, dyes are flown in the three vertically stacked compartments separated by transparent membranes (shown by the dotted rectangle).
Credit: Image courtesy of RTI International

Researchers at RTI International, in collaboration with the University of North Carolina at Chapel Hill, have developed a new lung-on-chip microdevice for laboratory studies of respiratory challenges and therapeutics. The microdevice includes multiple vertically stacked cellular layers that mimic the structure of the airway tissue.

Related Articles


The lung-on-a-chip model, funded by a contract from the Defense Threat Reduction Agency, was featured in an article published in Lab on a Chip.

"The development of this microfluidic lung model, as well as other organs-on-chip, holds the promise of improving the physiological relevance of cellular models for more accurate prediction of the effects of toxicants and drugs on humans, and for reducing the use of animals in medical and pharmaceutical research," said Sonia Grego, Ph.D., research scientist at RTI and the project's principal investigator.

The cellular model of the airway mucosa could provide insight into biological and pathophysiological effects that conventional cell cultures or animal models do not capture, and help lead to the development of new therapeutics.

Researchers leveraged microfabrication and microfluidic techniques to achieve a system with three vertically stacked fluidic microcompartments separated by nanoporous membranes. Researchers demonstrated that the device was able to support viable cultures of sensitive and physiologically representative primary cells instead of using the more common immortalized cell lines. The engineered cell culture enabled interaction between three cell types of the airways and reproduced their physiological interfaces -- becoming essentially an "organ-on-chip."

"The microfluidic cell cultures reproduced functions of the airway tissues such as mucus secretion and acted as a barrier to molecules," said Katelyn Sellgren, Ph.D., a postdoctoral research scientist at RTI and first author of the paper. "These properties are critical for inhalation toxicology and drug studies."

The conducting airways are involved in major diseases such as asthma and chronic obstructive lung diseases.

The research team included Scott H. Randell, Ph.D., a leading expert in the culture of primary lung cells, from the Cystic Fibrosis/Pulmonary Research and Treatment Center at the University of North Carolina at Chapel Hill.

"Our work demonstrated how a sophisticated investigation tool for the lung can be obtained," Grego said. "Next steps include studies ranging from exposure to environmental toxicants to pulmonary disease modeling and drug screening."


Story Source:

The above story is based on materials provided by RTI International. Note: Materials may be edited for content and length.


Journal Reference:

  1. Katelyn L. Sellgren, Elizabeth J. Butala, Brian P. Gilmour, Scott H. Randell, Sonia Grego. A biomimetic multicellular model of the airways using primary human cells. Lab on a Chip, 2014; 14 (17): 3349 DOI: 10.1039/C4LC00552J

Cite This Page:

RTI International. "Novel lung-on-a-chip developed." ScienceDaily. ScienceDaily, 14 August 2014. <www.sciencedaily.com/releases/2014/08/140814191229.htm>.
RTI International. (2014, August 14). Novel lung-on-a-chip developed. ScienceDaily. Retrieved March 1, 2015 from www.sciencedaily.com/releases/2014/08/140814191229.htm
RTI International. "Novel lung-on-a-chip developed." ScienceDaily. www.sciencedaily.com/releases/2014/08/140814191229.htm (accessed March 1, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Sunday, March 1, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Could a $34 Smartphone Device Improve HIV Diagnosis in Africa?

Could a $34 Smartphone Device Improve HIV Diagnosis in Africa?

Reuters - Innovations Video Online (Feb. 27, 2015) A dongle that plugs into a Smartphone mimics a lab-based blood test for HIV and syphilis and can detect the diseases in 15 minutes, say researchers. Tara Cleary reports. Video provided by Reuters
Powered by NewsLook.com
Doctor Says Head Transplants Possible Within Two Years

Doctor Says Head Transplants Possible Within Two Years

Buzz60 (Feb. 27, 2015) An Italian doctor is saying he could stick someone&apos;s head onto someone else&apos;s body. Patrick Jones (@Patrick_E_Jones) reports. Video provided by Buzz60
Powered by NewsLook.com
How Your Dentist Could Help Screen You For Diabetes

How Your Dentist Could Help Screen You For Diabetes

Newsy (Feb. 27, 2015) A new study from researchers at New York University suggests dentists could soon use blood samples taken from patients&apos; mouths to test for diabetes. Video provided by Newsy
Powered by NewsLook.com
The Best Tips to Makeover Your Health

The Best Tips to Makeover Your Health

Buzz60 (Feb. 27, 2015) If you&apos;re looking to boost your health this season, there are a few quick and easy steps to prompt you for success. Krystin Goodwin (@Krystingoodwin) has the best tips to give your health a makeover this spring! Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins