Featured Research

from universities, journals, and other organizations

Novel lung-on-a-chip developed

Date:
August 14, 2014
Source:
RTI International
Summary:
A new lung-on-chip microdevice for laboratory studies of respiratory challenges and therapeutics has been developed by scientists. The microdevice includes multiple vertically stacked cellular layers that mimic the structure of the airway tissue. The cellular model of the airway mucosa could provide insight into biological and pathophysiological effects that conventional cell cultures or animal models do not capture, and help lead to the development of new therapeutics.

RTI’s lung-on-a-chip emulates the multilayer airway tissue microarchitecture. In this picture, dyes are flown in the three vertically stacked compartments separated by transparent membranes (shown by the dotted rectangle).
Credit: Image courtesy of RTI International

Researchers at RTI International, in collaboration with the University of North Carolina at Chapel Hill, have developed a new lung-on-chip microdevice for laboratory studies of respiratory challenges and therapeutics. The microdevice includes multiple vertically stacked cellular layers that mimic the structure of the airway tissue.

The lung-on-a-chip model, funded by a contract from the Defense Threat Reduction Agency, was featured in an article published in Lab on a Chip.

"The development of this microfluidic lung model, as well as other organs-on-chip, holds the promise of improving the physiological relevance of cellular models for more accurate prediction of the effects of toxicants and drugs on humans, and for reducing the use of animals in medical and pharmaceutical research," said Sonia Grego, Ph.D., research scientist at RTI and the project's principal investigator.

The cellular model of the airway mucosa could provide insight into biological and pathophysiological effects that conventional cell cultures or animal models do not capture, and help lead to the development of new therapeutics.

Researchers leveraged microfabrication and microfluidic techniques to achieve a system with three vertically stacked fluidic microcompartments separated by nanoporous membranes. Researchers demonstrated that the device was able to support viable cultures of sensitive and physiologically representative primary cells instead of using the more common immortalized cell lines. The engineered cell culture enabled interaction between three cell types of the airways and reproduced their physiological interfaces -- becoming essentially an "organ-on-chip."

"The microfluidic cell cultures reproduced functions of the airway tissues such as mucus secretion and acted as a barrier to molecules," said Katelyn Sellgren, Ph.D., a postdoctoral research scientist at RTI and first author of the paper. "These properties are critical for inhalation toxicology and drug studies."

The conducting airways are involved in major diseases such as asthma and chronic obstructive lung diseases.

The research team included Scott H. Randell, Ph.D., a leading expert in the culture of primary lung cells, from the Cystic Fibrosis/Pulmonary Research and Treatment Center at the University of North Carolina at Chapel Hill.

"Our work demonstrated how a sophisticated investigation tool for the lung can be obtained," Grego said. "Next steps include studies ranging from exposure to environmental toxicants to pulmonary disease modeling and drug screening."


Story Source:

The above story is based on materials provided by RTI International. Note: Materials may be edited for content and length.


Journal Reference:

  1. Katelyn L. Sellgren, Elizabeth J. Butala, Brian P. Gilmour, Scott H. Randell, Sonia Grego. A biomimetic multicellular model of the airways using primary human cells. Lab on a Chip, 2014; 14 (17): 3349 DOI: 10.1039/C4LC00552J

Cite This Page:

RTI International. "Novel lung-on-a-chip developed." ScienceDaily. ScienceDaily, 14 August 2014. <www.sciencedaily.com/releases/2014/08/140814191229.htm>.
RTI International. (2014, August 14). Novel lung-on-a-chip developed. ScienceDaily. Retrieved September 21, 2014 from www.sciencedaily.com/releases/2014/08/140814191229.htm
RTI International. "Novel lung-on-a-chip developed." ScienceDaily. www.sciencedaily.com/releases/2014/08/140814191229.htm (accessed September 21, 2014).

Share This



More Health & Medicine News

Sunday, September 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Sierra Leone in Lockdown to Control Ebola

Sierra Leone in Lockdown to Control Ebola

AP (Sep. 21, 2014) Sierra Leone residents remained in lockdown on Saturday as part of a massive effort to confine millions of people to their homes in a bid to stem the biggest Ebola outbreak in history. (Sept. 20) Video provided by AP
Powered by NewsLook.com
Sierra Leone's Nationwide Ebola Curfew Underway

Sierra Leone's Nationwide Ebola Curfew Underway

Newsy (Sep. 20, 2014) Sierra Leone is locked down as aid workers and volunteers look for new cases of Ebola. Video provided by Newsy
Powered by NewsLook.com
Changes Found In Brain After One Dose Of Antidepressants

Changes Found In Brain After One Dose Of Antidepressants

Newsy (Sep. 19, 2014) A study suggest antidepressants can kick in much sooner than previously thought. Video provided by Newsy
Powered by NewsLook.com
Could Grief Affect The Immune Systems Of Senior Citizens?

Could Grief Affect The Immune Systems Of Senior Citizens?

Newsy (Sep. 19, 2014) The study found elderly people are much more likely to become susceptible to infection than younger adults going though a similar situation. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins